Date published: 2026-4-1

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Villin Antibody (B-12): sc-373997

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Datasheets
  • Villin Antibody (B-12) is a mouse monoclonal IgG2b κ, cited in 3 publications, provided at 200 µg/ml
  • raised against amino acids 721-780 mapping near the C-terminus of Villin of human origin
  • recommended for detection of Villin of human origin by WB, IP, IF and ELISA
  • See Villin (1D2C3): sc-58897 for Villin antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
  • m-IgG Fc BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Villin Antibody (B-12) for WB applications. These reagents are now offered in bundles with Villin Antibody (B-12) (see ordering information below).
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Villin Antibody (B-12) is a mouse monoclonal IgG2b antibody that detects Villin in human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Villin is a crucial actin-binding protein primarily found in the brush border of absorptive epithelial cells, where Villin plays a significant role in maintaining the structure and function of microvilli. Villin is essential for the organization of the actin cytoskeleton, which is vital for cell shape, motility, and the absorption of nutrients in the intestines. Villin′s ability to regulate actin filament dynamics is influenced by calcium ions, making Villin a key player in cellular responses to various stimuli. Anti-Villin antibody (B-12) provides a reliable tool for researchers studying Villin′s role in cellular processes and implications in health and disease, particularly in gastrointestinal disorders where microvilli integrity is compromised.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

Villin Antibody (B-12)

sc-373997
200 µg/ml
$322.00

Villin Antibody (B-12): m-IgG Fc BP-HRP Bundle

sc-537899
200 µg Ab; 10 µg BP
$361.00

Villin Antibody (B-12): m-IgGκ BP-HRP Bundle

sc-535362
200 µg Ab; 40 µg BP
$361.00

What is the recommended fixation method for immunofluorescence staining with Villin (B-12): sc-373997 monoclonal antibody?

Asked by: DefinitelyNotMatt
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-02-25
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Rated 5 out of 5 by from Superb immunofluorescence membrane stainingSuperb immunofluorescence membrane staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-04-29
Rated 5 out of 5 by from Nice Western Blot data of Villin expressionNice Western Blot data of Villin expression in HCT 8 whole cell lysate. -SCBT QC
Date published: 2014-03-22
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Villin Antibody (B-12) is rated 5.0 out of 5 by 2.
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