Villin Antibody (1D2C3): sc-58897
- Villin Antibody (1D2C3) is a mouse monoclonal IgG1 κ Villin antibody, cited in 81 publications, provided at 200 µg/ml
- raised against purified full length native Villin of chicken origin
- Villin Antibody (1D2C3) is recommended for detection of Villin of mouse, rat, human and avian origin by WB, IP, IF, IHC(P) and ELISA; non cross-reactive with breast cancer
- Anti-Villin Antibody (1D2C3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Villin Antibody (1D2C3) for WB and IHC(P) applications. These reagents are now offered in bundles with Villin Antibody (1D2C3) (see ordering information below).
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Villin Antibody (1D2C3) is a mouse monoclonal IgG1 kappa light chain antibody that detects Villin protein of mouse, rat, human, and avian origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Anti-Villin antibody (1D2C3) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Villin plays a crucial role in organizing actin cytoskeleton, particularly in brush border of absorptive epithelial cells, where Villin contributes to maintaining cell shape and surface area for absorption. Villin activity is regulated by calcium ions, which influence binding and severing of actin filaments, facilitating dynamic changes in cytoskeleton essential for cell motility and morphology. Villin interaction with other actin-binding proteins, such as Caldesmon, Filamin 1, and Nebulin, highlights Villin importance in cytoskeletal regulation, making Villin a vital component in both muscular and non-muscular tissues. Differential expression and regulation of Villin underscore roles in various cellular functions, emphasizing significance in maintaining cellular integrity and function across different species.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Villin Antibody (1D2C3) References:
- Developmental regulation of villin gene expression in the epithelial cell lineages of mouse digestive and urogenital tracts. | Maunoury, R., et al. 1992. Development. 115: 717-28. PMID: 1425351
- Caldesmon binds to smooth muscle myosin and myosin rod and crosslinks thick filaments to actin filaments. | Marston, S., et al. 1992. J Muscle Res Cell Motil. 13: 206-18. PMID: 1534566
- Villin expression is frequently lost in poorly differentiated colon cancer. | Arango, D., et al. 2012. Am J Pathol. 180: 1509-21. PMID: 22349300
- From the structure to the function of villin, an actin-binding protein of the brush border. | Friederich, E., et al. 1990. Bioessays. 12: 403-8. PMID: 2256904
- Site-specific phosphorylation of villin remodels the actin cytoskeleton to regulate Sendai viral glycoprotein-mediated membrane fusion. | Chandra, S., et al. 2019. FEBS Lett. 593: 1927-1943. PMID: 31183850
- Villin is a biomarker for reverse polarity in colorectal micropapillary carcinoma. | Zhao, L., et al. 2021. Oncol Lett. 21: 72. PMID: 33365083
- Actin-binding and dimerization domains of HeLa cell filamin. | Weihing, RR. 1988. Biochemistry. 27: 1865-9. PMID: 3378035
- Villin expression in human tumors: a tissue microarray study on 14,398 tumors. | Dum, D., et al. 2022. Expert Rev Mol Diagn. 22: 665-675. PMID: 35866621
- Villin-1 regulates ferroptosis in colorectal cancer progression. | Hu, B., et al. 2024. FEBS J.. PMID: 39658274
- The complete primary structure of human nebulin and its correlation to muscle structure. | Labeit, S. and Kolmerer, B. 1995. J Mol Biol. 248: 308-15. PMID: 7739042
- Multiple-sited interaction of caldesmon with Ca(2+)-calmodulin. | Huber, PA., et al. 1996. Biochem J. 316 (Pt 2): 413-20. PMID: 8687382
- cDNA cloning of mouse nebulin. Evidence that the nebulin-coding sequence is highly conserved among vertebrates. | Zhang, JQ., et al. 1996. Eur J Biochem. 239: 835-41. PMID: 8774733
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Villin Antibody (1D2C3) | sc-58897 | 200 µg/ml | $322.00 | |||
Villin Antibody (1D2C3): m-IgG Fc BP-HRP Bundle | sc-528631 | 200 µg Ab; 10 µg BP | $361.00 | |||
Villin Antibody (1D2C3): m-IgGκ BP-HRP Bundle | sc-521061 | 200 µg Ab, 40 µg BP | $361.00 | |||
Villin Antibody (1D2C3): m-IgG1 BP-HRP Bundle | sc-543041 | 200 µg Ab; 20 µg BP | $361.00 | |||
Villin Antibody (1D2C3) AC | sc-58897 AC | 500 µg/ml, 25% agarose | $424.00 | |||
Villin Antibody (1D2C3) HRP | sc-58897 HRP | 200 µg/ml | $322.00 | |||
Villin Antibody (1D2C3) FITC | sc-58897 FITC | 200 µg/ml | $336.00 | |||
Villin Antibody (1D2C3) PE | sc-58897 PE | 200 µg/ml | $349.00 | |||
Villin Antibody (1D2C3) Alexa Fluor® 488 | sc-58897 AF488 | 200 µg/ml | $364.00 | |||
Villin Antibody (1D2C3) Alexa Fluor® 546 | sc-58897 AF546 | 200 µg/ml | $364.00 | |||
Villin Antibody (1D2C3) Alexa Fluor® 594 | sc-58897 AF594 | 200 µg/ml | $364.00 | |||
Villin Antibody (1D2C3) Alexa Fluor® 647 | sc-58897 AF647 | 200 µg/ml | $364.00 | |||
Villin Antibody (1D2C3) Alexa Fluor® 680 | sc-58897 AF680 | 200 µg/ml | $364.00 | |||
Villin Antibody (1D2C3) Alexa Fluor® 790 | sc-58897 AF790 | 200 µg/ml | $364.00 |