Vigilin Antibody (H-3): sc-271523
- Vigilin Antibody (H-3) is a mouse monoclonal IgG2a κ Vigilin antibody, cited in 1 publications, provided at 200 µg/ml
- raised against amino acids 126-290 mapping near the N-terminus of Vigilin of human origin
- Vigilin Antibody (H-3) is recommended for detection of Vigilin of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-Vigilin Antibody (H-3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Vigilin Antibody (H-3) for WB applications. These reagents are now offered in bundles with Vigilin Antibody (H-3) (see ordering information below).
QUICK LINKS
SEE ALSO...
Vigilin Antibody (H-3) is a mouse monoclonal IgG2a kappa light chain antibody that detects Vigilin protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-Vigilin antibody (H-3) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Vigilin, a K homology (KH) protein, is predominantly located in the nucleus and cytoplasm of all eukaryotic species, where its unique structure, comprising 14 to 15 consecutively arranged KH domains, plays a critical role in mediating RNA-protein interactions. This structural configuration is essential for Vigilin′s function in heterochromatin formation and cytoplasmic mRNA decay, making Vigilin a valuable marker for assessing translational activity. The gene encoding Vigilin, located on chromosome 2q37, is vital for cell viability, and Vigilin expression is upregulated by intracellular cholesterol levels. Notably, Vigilin interacts with the 80S ribosome through its C-terminal domain, linking tRNA export and the channeled tRNA cycle on ribosomes, which underscores its importance in cellular processes related to protein synthesis and lipid metabolism.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Vigilin Antibody (H-3) References:
- Vigilin binding selectively inhibits cleavage of the vitellogenin mRNA 3'-untranslated region by the mRNA endonuclease polysomal ribonuclease 1. | Cunningham, KS., et al. 2000. Proc Natl Acad Sci U S A. 97: 12498-502. PMID: 11050168
- Cloning and expression of a cellular high density lipoprotein-binding protein that is up-regulated by cholesterol loading of cells. | McKnight, GL., et al. 1992. J Biol Chem. 267: 12131-41. PMID: 1318310
- The human vigilin gene: identification, chromosomal localization and expression pattern. | Plenz, G., et al. 1994. Hum Genet. 93: 575-82. PMID: 8168838
- Localization of the gene for high-density lipoprotein binding protein (HDLBP) to human chromosome 2q37. | Xia, YR., et al. 1993. Genomics. 16: 524-5. PMID: 8390966
- Vigilin contains a functional nuclear localisation sequence and is present in both the cytoplasm and the nucleus. | Kügler, S., et al. 1996. FEBS Lett. 382: 330-4. PMID: 8605996
- Two additional 5' exons in the human Vigilin gene distinguish it from the chicken gene and provide the structural basis for differential routes of gene expression. | Kügler, S., et al. 1996. Eur J Biochem. 238: 410-7. PMID: 8681952
- Vigilin, a ubiquitous protein with 14 K homology domains, is the estrogen-inducible vitellogenin mRNA 3'-untranslated region-binding protein. | Dodson, RE. and Shapiro, DJ. 1997. J Biol Chem. 272: 12249-52. PMID: 9139664
- In vitro genetic analysis of the RNA binding site of vigilin, a multi-KH-domain protein. | Kanamori, H., et al. 1998. Mol Cell Biol. 18: 3991-4003. PMID: 9632784
- High-density lipoprotein-binding protein (HBP)/vigilin is expressed in human atherosclerotic lesions and colocalizes with apolipoprotein E. | Chiu, DS., et al. 1997. Arterioscler Thromb Vasc Biol. 17: 2350-8. PMID: 9409201
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Vigilin Antibody (H-3) | sc-271523 | 200 µg/ml | $322.00 | |||
Vigilin Antibody (H-3): m-IgG Fc BP-HRP Bundle | sc-529201 | 200 µg Ab; 10 µg BP | $361.00 | |||
Vigilin Antibody (H-3): m-IgGκ BP-HRP Bundle | sc-521968 | 200 µg Ab, 40 µg BP | $361.00 | |||
Vigilin Antibody (H-3): m-IgG2a BP-HRP Bundle | sc-547276 | 200 µg Ab; 10 µg BP | $361.00 | |||
Vigilin Antibody (H-3) AC | sc-271523 AC | 500 µg/ml, 25% agarose | $424.00 | |||
Vigilin Antibody (H-3) HRP | sc-271523 HRP | 200 µg/ml | $322.00 | |||
Vigilin Antibody (H-3) FITC | sc-271523 FITC | 200 µg/ml | $336.00 | |||
Vigilin Antibody (H-3) PE | sc-271523 PE | 200 µg/ml | $349.00 | |||
Vigilin Antibody (H-3) Alexa Fluor® 488 | sc-271523 AF488 | 200 µg/ml | $364.00 | |||
Vigilin Antibody (H-3) Alexa Fluor® 546 | sc-271523 AF546 | 200 µg/ml | $364.00 | |||
Vigilin Antibody (H-3) Alexa Fluor® 594 | sc-271523 AF594 | 200 µg/ml | $364.00 | |||
Vigilin Antibody (H-3) Alexa Fluor® 647 | sc-271523 AF647 | 200 µg/ml | $364.00 | |||
Vigilin Antibody (H-3) Alexa Fluor® 680 | sc-271523 AF680 | 200 µg/ml | $364.00 | |||
Vigilin Antibody (H-3) Alexa Fluor® 790 | sc-271523 AF790 | 200 µg/ml | $364.00 |