VEGF-C Antibody (E-6) recommended for detection of precursor and mature VEGF-C of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

VEGF-C Antibody (E-6): sc-374628

VEGF-C Antibody (E-6) is rated 4.3 out of 5 by 11.
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Datasheets

    • Anti-VEGF-C Antibody (E-6) is a mouse monoclonal IgG2a VEGF-C antibody provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 103-137 near the N-terminus of VEGF-C of human origin
    • Anti-VEGF-C Antibody (E-6) is recommended for detection of precursor and mature VEGF-C of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-VEGF-C Antibody (E-6) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • blocking peptide, sc-374628 P
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of VEGF-C (E-6): sc-374628.
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Does this antibody have any neutralisation or inhibitory potential in any cell-based assays?

Asked by: Steven91
Thank you for your question. No, this antibody has not been tested for any neutralization or inhibitory use, so we do not have that information. It is recommended for detection of VEGF-C via WB, IP, PAR and ELISA.
Answered by: Tech Service
Date published: 2019-01-14

PMID: # 25796380, This Manuscript has not shown VEGFC expression by western blot. Please correct me if I am wrong.

Asked by: sdas128
Thank you for your question. This journal article is listed as a product citation for our mouse monoclonal antibody, VEGF-C (E-6): sc-374628, because this specific product catalog number and clone name were referenced in the materials and methods of the article. "Immunodetection of VEGF...was done using mouse monoclonal anti-VEGF antibody (1:1000, E-6, sc-374628; Santa Cruz Biotech, Santa Cruz...".
Answered by: Technical Support
Date published: 2017-05-15

What is the recommended secondary reagent for VEGF-C Antibody (E-6): sc-374628 in Western blot when working with human samples?

Asked by: Trav11
Thank you for your question. We recommend using one of our exclusive Mouse IgG Binding Proteins as a secondary detection reagent, such as m-IgGκ BP-HRP: sc-516102, for Western blot. A complete list of all available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety If you have any further questions or concerns, please feel free to contact our Technical Service department by calling 800-457-8301 option 2, emailing [email protected], or using the Live Chat function on our website.
Answered by: Technical Support 7
Date published: 2017-02-03
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Rated 4 out of 5 by from works well for WB and IF Shows a specific signal in WB (dilution 1:200 in BSA/TBS-T) at 40kDa for HEp-2, RKO and LoVo whole cell Lysates. When used in IF, the cells (HEp-2) were fixed in 2% formaldehyde and the dilution was adjusted to 1:50 - IF signal was cytoplasmic but still slightly diffuse
Date published: 2020-01-09
Rated 4 out of 5 by from Works well on lymphatic staining This antibody works well on lymphatic staining. Beautiful macrophages staining.
Date published: 2019-10-09
Rated 5 out of 5 by from Excelent antibody for western blotting I obtained really strong signal by western blotting, using a 1:500 dilution of this antibody in glioblastoma cell lines
Date published: 2019-06-27
Rated 5 out of 5 by from great features Strip is very noticeable, quality is very good,thank you very much
Date published: 2017-09-05
Rated 1 out of 5 by from unexpected protein size I tested sample with the lysates from mouse thymic epithelial cell lines and got non-specific band around 20kda. Conditions of WB ; 4-20% SDS-PAGE, Wet transfer (400mA/2hr), 1:200 primary antibody in 5% TBST.
Date published: 2017-08-31
Rated 5 out of 5 by from Great IF image We used this antibody for IF staining and got a very clean image showing great HeLa cells
Date published: 2017-01-09
Rated 5 out of 5 by from Highly potent and very specific antibody This monoclonal antibody was independently tested in-house by Kinexus Bioinformatics Corporation and validated for use for Western blotting. There was extremely strong detection of the ~40/80 kDa target proteins in mouse tissues (brain/testes/thymus/muscle mix). No major cross-reactivities were observed at 2 µg/ml antibody concentration with off-target proteins in the mouse tissues tested, except for three other very strongly cross-reactive proteins.
Date published: 2016-11-10
Rated 5 out of 5 by from Great for western blotting! Used this for western blotting on kidney lysates- worked great. Better than other VEGF-C antibodies I have tried.
Date published: 2016-11-04
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