Anti-VEGF-C Antibody (E-6) is a mouse monoclonal IgG2aVEGF-C antibody provided at 200 µg/ml
specific for an epitope mapping between amino acids 103-137 near the N-terminus of VEGF-C of human origin
Anti-VEGF-C Antibody (E-6) is recommended for detection of precursor and mature VEGF-C of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
Anti-VEGF-C Antibody (E-6) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
blocking peptide, sc-374628 P
See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of VEGF-C (E-6): sc-374628.
Every item is shipped based on the best shipping method assessed for the temperature requirements of that specific item. Items are grouped and shipped together whenever
possible, and a separate shipping charge will be included for each shipping method required. Shipping charges listed below are from our US warehouses to the Contiguous US,
Alaska, Hawaii, Canada and Puerto Rico. Shipping charges for countries outside the US and Canada will be determined once order has been received
Please note: We can not ship to PO boxes
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Ships via FedEx Ground to Contiguous US, Alaska, Canada, Monday through Friday. This method is used for less temperature sensitive items such as lab ware and animal
health products, bulky and/or heavy items
Labware ships FedEx Ground free of charge to the contiguous US
VEGF-C Antibody (E-6) is a high quality monoclonal VEGF-C antibody (also designated VEGF-C antibody) suitable for the detection of the VEGF-C protein of mouse, rat and human origin. VEGF-C Antibody (E-6) is available as both the non-conjugated anti-VEGF-C antibody form, as well as multiple conjugated forms of anti-VEGF-C antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. The onset of angiogenesis is believed to be an early event in tumorigenesis and may facilitate tumor progression and metastasis. Several growth factors with angiogenic activity have been described. These include fibroblast growth factor (FGF), platelet derived growth factor (PDGF) and vascular endothelial growth factor (VEGF). VEGF is a dimeric glycoprotein with structural homology to PDGF. Several variants of VEGF have been described that arise by alternative mRNA splicing. It has been speculated that VEGF may function as a tumor angiogenesis factor in vivo. Two additional proteins, designated VEGF-B and VEGF-C, share a significant degree of homology with VEGF. VEGF-B is abundantly expressed in heart and skeletal muscle and is frequently coexpressed with VEGF. VEGF-C binds to and specifically activates Flt-4 and Flk-1. The genes that encode VEGF-B and VEGF-C have been localized to chromosomes 11q13.1 and 4q34.3, respectively.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Does this antibody have any neutralisation or inhibitory potential in any cell-based assays?
Asked by: Steven91
Thank you for your question. No, this antibody has not been tested for any neutralization or inhibitory use, so we do not have that information. It is recommended for detection of VEGF-C via WB, IP, PAR and ELISA.
Answered by: Tech Service
Date published: 2019-01-14
PMID: # 25796380, This Manuscript has not shown VEGFC expression by western blot. Please correct me if I am wrong.
Asked by: sdas128
Thank you for your question. This journal article is listed as a product citation for our mouse monoclonal antibody, VEGF-C (E-6): sc-374628, because this specific product catalog number and clone name were referenced in the materials and methods of the article. "Immunodetection of VEGF...was done using mouse monoclonal anti-VEGF antibody (1:1000, E-6, sc-374628; Santa Cruz Biotech, Santa Cruz...".
Answered by: Technical Support
Date published: 2017-05-15
What is the recommended secondary reagent for VEGF-C Antibody (E-6): sc-374628 in Western blot when working with human samples?
Asked by: Trav11
Thank you for your question. We recommend using one of our exclusive Mouse IgG Binding Proteins as a secondary detection reagent, such as m-IgGκ BP-HRP: sc-516102, for Western blot. A complete list of all available binding proteins is available on our website here:
If you have any further questions or concerns, please feel free to contact our Technical Service department by calling 800-457-8301 option 2, emailing [email protected], or using the Live Chat function on our website.
Rated 4 out of
works well for WB and IFShows a specific signal in WB (dilution 1:200 in BSA/TBS-T) at 40kDa for HEp-2, RKO and LoVo whole cell Lysates. When used in IF, the cells (HEp-2) were fixed in 2% formaldehyde and the dilution was adjusted to 1:50 - IF signal was cytoplasmic but still slightly diffuse
Date published: 2020-01-09
Rated 4 out of
Works well on lymphatic stainingThis antibody works well on lymphatic staining. Beautiful macrophages staining.
Date published: 2019-10-09
Rated 5 out of
Excelent antibody for western blottingI obtained really strong signal by western blotting, using a 1:500 dilution of this antibody in glioblastoma cell lines
Date published: 2019-06-27
Rated 5 out of
Yubin Feng from
great featuresStrip is very noticeable， quality is very good，thank you very much
Date published: 2017-09-05
Rated 1 out of
unexpected protein sizeI tested sample with the lysates from mouse thymic epithelial cell lines and got non-specific band around 20kda. Conditions of WB ; 4-20% SDS-PAGE, Wet transfer (400mA/2hr), 1:200 primary antibody in 5% TBST.
Date published: 2017-08-31
Rated 5 out of
Randy McDonald from
Great IF imageWe used this antibody for IF staining and got a very clean image showing great HeLa cells
Date published: 2017-01-09
Rated 5 out of
Steven Pelech at Kinexus from
Highly potent and very specific antibodyThis monoclonal antibody was independently tested in-house by Kinexus Bioinformatics Corporation and validated for use for Western blotting. There was extremely strong detection of the ~40/80 kDa target proteins in mouse tissues (brain/testes/thymus/muscle mix). No major cross-reactivities were observed at 2 µg/ml antibody concentration with off-target proteins in the mouse tissues tested, except for three other very strongly cross-reactive proteins.
Date published: 2016-11-10
Rated 5 out of
Great for western blotting!Used this for western blotting on kidney lysates- worked great. Better than other VEGF-C antibodies I have tried.
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