Vasa Antibody (C-2): sc-514249

Vasa Antibody (C-2) is a mouse monoclonal IgM kappa antibody that detects Vasa protein of Drosophila origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Vasa (C-2) antibody is available in both conjugated and non-conjugated forms, providing versatility for various experimental needs. VASA, a 724 amino acid ATP-dependent RNA helicase belonging to the DEAD-box family, plays a crucial role in germline development by regulating the translation of specific mRNAs essential for differentiation. VASA expression is tightly regulated, being specifically found in germline cells throughout the life cycle while remaining undetectable in somatic tissues. In vertebrates, VASA is primarily restricted to bisexually reproducing organisms, highlighting VASA′s importance in reproductive biology. VASA is predominantly cytoplasmic and is localized in migratory primordial germ cells within the gonadal ridge, where VASA is vital for proper germ cell development. In testicular sections, VASA expression is most pronounced in spermatogonia, gradually decreasing in spermatocytes and spermatids, and is absent in mature sperm. In the ovary, VASA is highly expressed in oogonia and continues to be present throughout oogenesis. VASA features a glycine-rich N-terminus with multiple repeats of an RGG motif, which facilitates RNA binding, underscoring VASA′s functional significance in the regulation of mRNA translation during germ cell differentiation.

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Vasa Antibody (C-2) References:

1. The human VASA gene is specifically expressed in the germ cell lineage.  |  Castrillon, DH., et al. 2000. Proc Natl Acad Sci U S A. 97: 9585-90. PMID: 10920202
2. Spatio-temporal expression of the germ cell marker genes MATER, ZAR1, GDF9, BMP15,andVASA in adult bovine tissues, oocytes, and preimplantation embryos.  |  Pennetier, S., et al. 2004. Biol Reprod. 71: 1359-66. PMID: 15189828
3. Pathobiological implications of the expression of markers of testicular carcinoma in situ by fetal germ cells.  |  Honecker, F., et al. 2004. J Pathol. 203: 849-57. PMID: 15221945
4. Differentiation and development of human female germ cells during prenatal gonadogenesis: an immunohistochemical study.  |  Stoop, H., et al. 2005. Hum Reprod. 20: 1466-76. PMID: 15734757
5. Differential expression of vasa RNA and protein during spermatogenesis and oogenesis in the gibel carp (Carassius auratus gibelio), a bisexually and gynogenetically reproducing vertebrate.  |  Xu, H., et al. 2005. Dev Dyn. 233: 872-82. PMID: 15880437
6. RNA helicases: regulators of differentiation.  |  Abdelhaleem, M. 2005. Clin Biochem. 38: 499-503. PMID: 15885226
7. Bent out of shape: RNA unwinding by the DEAD-box helicase Vasa.  |  Linder, P. and Lasko, P. 2006. Cell. 125: 219-21. PMID: 16630807
8. Characterization of a vasa-like gene from the pacific white shrimp Litopenaeus vannamei and its expression during oogenesis.  |  Aflalo, ED., et al. 2007. Mol Reprod Dev. 74: 172-7. PMID: 16955407

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Vasa Antibody (C-2) has 0 citations in a variety of scientific publications.

Primary antibodies for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Vasa Antibody (C-2) Specifications

Alternate Product Name: Vasa

Product Clone Name: C-2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgM kappa light chain

Amino Acids: 4-21

Epitope Target: Vasa

Epitope Species: Drosophila melanogaster

Product Reactivity/Detection: Vasa

Species Reactivity/Detection: d

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgM kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for Vasa Antibody (C-2): AC: Agarose, AF488: Alexa Fluor^® 488, AF647: Alexa Fluor^® 647, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin