VapC Antibody (B-9): sc-390325
- VapC Antibody (B-9) is a mouse monoclonal IgG2b κ provided at 200 µg/ml
- raised against amino acids 29-174 mapping at the C-terminus of virulence associated protein VapC of Rhodococcus equi (strain: 103gb-synonym: Corynebacterium equiold-name: Rhodococcus equi) origin
- VapC Antibody (B-9) is recommended for detection of virulence associated protein VapC by WB, IP, IF and ELISA
- Anti-VapC Antibody (B-9) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for VapC Antibody (B-9) for WB applications. These reagents are now offered in bundles with VapC Antibody (B-9) (see ordering information below).
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VapC Antibody (B-9) is a mouse monoclonal IgG2b kappa light chain antibody that detects VapC protein across various species through western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). VapC (B-9) antibody comes in non-conjugated form and multiple conjugated versions, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and various Alexa Fluor® conjugates, offering flexibility for different experimental applications. VapC functions as a bacterial toxin that inhibits host cell translation, contributing to bacterial virulence and pathogenicity during infections. VapC research is crucial for understanding bacterial defense evasion mechanisms and identifying potential therapeutic targets in infectious diseases. VapC interactions with VAMP-associated proteins (VAPs) play a key role in SNARE-mediated membrane fusion processes, essential for intracellular transport and communication. VAP gene mutations, including VAP-B and VAP-C, have links to severe neurodegenerative disorders, highlighting VapC′s significance in both microbial pathogenesis and human health.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
VapC Antibody (B-9) References:
- DNA sequence and comparison of virulence plasmids from Rhodococcus equi ATCC 33701 and 103. | Takai, S., et al. 2000. Infect Immun. 68: 6840-7. PMID: 11083803
- Immunoglobulin G subisotype responses of pneumonic and healthy, exposed foals and adult horses to Rhodococcus equi virulence-associated proteins. | Hooper-McGrevy, KE., et al. 2003. Clin Diagn Lab Immunol. 10: 345-51. PMID: 12738629
- Deletion of vapA encoding Virulence Associated Protein A attenuates the intracellular actinomycete Rhodococcus equi. | Jain, S., et al. 2003. Mol Microbiol. 50: 115-28. PMID: 14507368
- Rhodococcus equi secreted antigens are immunogenic and stimulate a type 1 recall response in the lungs of horses immune to R. equi infection. | Kohler, AK., et al. 2003. Infect Immun. 71: 6329-37. PMID: 14573652
- The LysR-type transcriptional regulator VirR is required for expression of the virulence gene vapA of Rhodococcus equi ATCC 33701. | Russell, DA., et al. 2004. J Bacteriol. 186: 5576-84. PMID: 15317761
- VapI, a new member of the Rhodococcus equi Vap family. | Polidori, M. and Haas, A. 2006. Antonie Van Leeuwenhoek. 90: 299-304. PMID: 16871422
- Molecular characterization of Rhodococcus equi from horse-breeding farms by means of multiplex PCR for the vap gene family. | Monego, F., et al. 2009. Curr Microbiol. 58: 399-403. PMID: 19205798
- Development of a live, attenuated, potential vaccine strain of R. equi expressing vapA and the virR operon, and virulence assessment in the mouse. | Whitehead, AE., et al. 2012. Vet Immunol Immunopathol. 145: 479-84. PMID: 22088674
- Development of ELISA test for determination of the level of antibodies against Rhodococcus equi in equine serum and colostrum. | Witkowski, L., et al. 2012. Vet Immunol Immunopathol. 149: 280-5. PMID: 22883938
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
VapC Antibody (B-9) | sc-390325 | 200 µg/ml | $322.00 | |||
VapC Antibody (B-9): m-IgG Fc BP-HRP Bundle | sc-530126 | 200 µg Ab; 10 µg BP | $361.00 | |||
VapC Antibody (B-9): m-IgGκ BP-HRP Bundle | sc-523490 | 200 µg Ab, 40 µg BP | $361.00 | |||
VapC Antibody (B-9): m-IgG2b BP-HRP Bundle | sc-550167 | 200 µg Ab; 10 µg BP | $361.00 | |||
VapC Antibody (B-9) AC | sc-390325 AC | 500 µg/ml, 25% agarose | $424.00 | |||
VapC Antibody (B-9) HRP | sc-390325 HRP | 200 µg/ml | $322.00 | |||
VapC Antibody (B-9) FITC | sc-390325 FITC | 200 µg/ml | $336.00 | |||
VapC Antibody (B-9) PE | sc-390325 PE | 200 µg/ml | $349.00 | |||
VapC Antibody (B-9) Alexa Fluor® 488 | sc-390325 AF488 | 200 µg/ml | $364.00 | |||
VapC Antibody (B-9) Alexa Fluor® 546 | sc-390325 AF546 | 200 µg/ml | $364.00 | |||
VapC Antibody (B-9) Alexa Fluor® 594 | sc-390325 AF594 | 200 µg/ml | $364.00 | |||
VapC Antibody (B-9) Alexa Fluor® 647 | sc-390325 AF647 | 200 µg/ml | $364.00 | |||
VapC Antibody (B-9) Alexa Fluor® 680 | sc-390325 AF680 | 200 µg/ml | $364.00 | |||
VapC Antibody (B-9) Alexa Fluor® 790 | sc-390325 AF790 | 200 µg/ml | $364.00 |