ValRS Antibody (D-7): sc-166674
- ValRS Antibody (D-7) is a mouse monoclonal IgG2a κ ValRS antibody provided at 200 µg/ml
- raised against amino acids 1-203 mapping at the N-terminus of ValRS of human origin
- ValRS Antibody (D-7) is recommended for detection of ValRS of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-ValRS Antibody (D-7) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for ValRS Antibody (D-7) for WB applications. These reagents are now offered in bundles with ValRS Antibody (D-7) (see ordering information below).
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ValRS Antibody (D-7) is a mouse monoclonal IgG2a kappa light chain antibody that detects ValRS protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). ValRS (D-7) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. ValRS, or valyl-tRNA synthetase, is a crucial enzyme in protein synthesis, responsible for accurately attaching valine to corresponding tRNA(Val). This specificity ensures correct protein synthesis, maintaining cellular function and integrity. ValRS belongs to the class-I aminoacyl-tRNA synthetase family, which includes related enzymes like leucyl-tRNA synthetase and isoleucyl-tRNA synthetase. These enzymes are large monomeric proteins catalyzing tRNA aminoacylation, a critical step in translating genetic information into functional proteins. ValRS operates through two active sites: the synthetic active site, joining valine to tRNA(Val), and the CP1 editing active site, hydrolyzing incorrectly attached tRNA(Thr) to prevent synthesis errors. ValRS also interacts with elongation factor 1 (EF-1) to facilitate aminoacyl-tRNA transfer during translation, and ValRS activity may be modulated by phosphorylation through protein kinase C, highlighting ValRS importance in protein synthesis regulation.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
ValRS Antibody (D-7) References:
- Mechanism of molecular interactions for tRNA(Val) recognition by valyl-tRNA synthetase. | Fukai, S., et al. 2003. RNA. 9: 100-11. PMID: 12554880
- Identification of functional similarities between proteins using directed evolution. | Christ, D. and Winter, G. 2003. Proc Natl Acad Sci U S A. 100: 13202-6. PMID: 14573700
- Transiently misacylated tRNA is a primer for editing of misactivated adenylates by class I aminoacyl-tRNA synthetases. | Nordin, BE. and Schimmel, P. 2003. Biochemistry. 42: 12989-97. PMID: 14596614
- Crystal structure of leucyl-tRNA synthetase from the archaeon Pyrococcus horikoshii reveals a novel editing domain orientation. | Fukunaga, R. and Yokoyama, S. 2005. J Mol Biol. 346: 57-71. PMID: 15663927
- Breaking the stereo barrier of amino acid attachment to tRNA by a single nucleotide. | Shitivelband, S. and Hou, YM. 2005. J Mol Biol. 348: 513-21. PMID: 15826650
- Structural basis for non-cognate amino acid discrimination by the valyl-tRNA synthetase editing domain. | Fukunaga, R. and Yokoyama, S. 2005. J Biol Chem. 280: 29937-45. PMID: 15970591
- Three-dimensional reconstruction of the valyl-tRNA synthetase/elongation factor-1H complex and localization of the delta subunit. | Jiang, S., et al. 2005. FEBS Lett. 579: 6049-54. PMID: 16229838
- A present-day aminoacyl-tRNA synthetase with ancestral editing properties. | Zhu, B., et al. 2007. RNA. 13: 15-21. PMID: 17095543
- Isolated CP1 domain of Escherichia coli leucyl-tRNA synthetase is dependent on flanking hinge motifs for amino acid editing activity. | Betha, AK., et al. 2007. Biochemistry. 46: 6258-67. PMID: 17474713
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ValRS Antibody (D-7) | sc-166674 | 200 µg/ml | $316.00 | |||
ValRS Antibody (D-7): m-IgG Fc BP-HRP Bundle | sc-529027 | 200 µg Ab; 10 µg BP | $354.00 | |||
ValRS Antibody (D-7): m-IgGκ BP-HRP Bundle | sc-521704 | 200 µg Ab, 40 µg BP | $354.00 | |||
ValRS Antibody (D-7): m-IgG2a BP-HRP Bundle | sc-552105 | 200 µg Ab; 10 µg BP | $354.00 | |||
ValRS Antibody (D-7) AC | sc-166674 AC | 500 µg/ml, 25% agarose | $416.00 | |||
ValRS Antibody (D-7) HRP | sc-166674 HRP | 200 µg/ml | $316.00 | |||
ValRS Antibody (D-7) FITC | sc-166674 FITC | 200 µg/ml | $330.00 | |||
ValRS Antibody (D-7) PE | sc-166674 PE | 200 µg/ml | $343.00 | |||
ValRS Antibody (D-7) Alexa Fluor® 488 | sc-166674 AF488 | 200 µg/ml | $357.00 | |||
ValRS Antibody (D-7) Alexa Fluor® 546 | sc-166674 AF546 | 200 µg/ml | $357.00 | |||
ValRS Antibody (D-7) Alexa Fluor® 594 | sc-166674 AF594 | 200 µg/ml | $357.00 | |||
ValRS Antibody (D-7) Alexa Fluor® 647 | sc-166674 AF647 | 200 µg/ml | $357.00 | |||
ValRS Antibody (D-7) Alexa Fluor® 680 | sc-166674 AF680 | 200 µg/ml | $357.00 | |||
ValRS Antibody (D-7) Alexa Fluor® 790 | sc-166674 AF790 | 200 µg/ml | $357.00 |