VAC14 Antibody (F-12): sc-365272
- VAC14 Antibody (F-12) is a mouse monoclonal IgG1 κ provided at 200 µg/ml
- raised against amino acids 421-681 mapping within an internal region of VAC14 of human origin
- Anti-VAC14 Antibody (F-12) is recommended for detection of VAC14 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-VAC14 Antibody (F-12) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP is the preferred secondary detection reagent for VAC14 Antibody (F-12) for WB and IHC(P) applications. This reagent is now offered in a bundle with VAC14 Antibody (F-12) (see ordering information below).
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VAC14 Antibody (F-12) is a mouse monoclonal IgG1 kappa light chain antibody that detects VAC14 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Anti-VAC14 antibody (F-12) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. VAC14, also known as TAX1BP2 (Tax1-binding protein 2) or TRX, is a crucial component in regulating phosphatidylinositol 3,5-bisphosphate (PI(3,5)P2), a signaling molecule that plays a vital role in cellular compartmentalization and membrane dynamics. Located in the cytoplasm, VAC14 interacts with FIG4 to modulate PI(3,5)P2 synthesis, which is essential for various intracellular trafficking processes and maintaining cellular homeostasis. VAC14 consists of 782 amino acids and features six HEAT repeats, which are important for protein-protein interactions and forming regulatory complexes. VAC14 is situated on human chromosome 16, a region that encompasses over 900 genes and represents nearly 3% of the human genome, highlighting VAC14′s significance in cellular function and signaling pathways.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
VAC14 Antibody (F-12) References:
- Vac14 controls PtdIns(3,5)P(2) synthesis and Fab1-dependent protein trafficking to the multivesicular body. | Dove, SK., et al. 2002. Curr Biol. 12: 885-93. PMID: 12062051
- VAC14 nucleates a protein complex essential for the acute interconversion of PI3P and PI(3,5)P(2) in yeast and mouse. | Jin, N., et al. 2008. EMBO J. 27: 3221-34. PMID: 19037259
- Modulation of synaptic function by VAC14, a protein that regulates the phosphoinositides PI(3,5)P₂ and PI(5)P. | Zhang, Y., et al. 2012. EMBO J. 31: 3442-56. PMID: 22842785
- Vac14 protein multimerization is a prerequisite step for Fab1 protein complex assembly and function. | Alghamdi, TA., et al. 2013. J Biol Chem. 288: 9363-72. PMID: 23389034
- Biallelic Mutations of VAC14 in Pediatric-Onset Neurological Disease. | Lenk, GM., et al. 2016. Am J Hum Genet. 99: 188-94. PMID: 27292112
- Nbeal2 interacts with Dock7, Sec16a, and Vac14. | Mayer, L., et al. 2018. Blood. 131: 1000-1011. PMID: 29187380
- Arabidopsis VAC14 Is Critical for Pollen Development through Mediating Vacuolar Organization. | Zhang, WT., et al. 2018. Plant Physiol. 177: 1529-1538. PMID: 29884680
- VAC14 Gene-Related Parkinsonism-Dystonia With Response to Deep Brain Stimulation. | de Gusmao, CM., et al. 2019. Mov Disord Clin Pract. 6: 494-497. PMID: 31392254
- Expanding the spectrum of VAC14 related pediatric-onset neurological disease; striatonigral degeneration with brainstem involvement. | Karaoğlu, P. and Köse, M. 2021. Eur J Med Genet. 64: 104117. PMID: 33248288
- TIG1 Inhibits the mTOR Signaling Pathway in Malignant Melanoma Through the VAC14 Protein. | Wang, CH., et al. 2023. Anticancer Res. 43: 2635-2643. PMID: 37247911
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
VAC14 Antibody (F-12) | sc-365272 | 200 µg/ml | $322.00 | |||
VAC14 Antibody (F-12): m-IgG Fc BP-HRP Bundle | sc-525871 | 200 µg Ab; 10 µg BP | $361.00 | |||
VAC14 Antibody (F-12) AC | sc-365272 AC | 500 µg/ml, 25% agarose | $424.00 | |||
VAC14 Antibody (F-12) HRP | sc-365272 HRP | 200 µg/ml | $322.00 | |||
VAC14 Antibody (F-12) FITC | sc-365272 FITC | 200 µg/ml | $336.00 | |||
VAC14 Antibody (F-12) PE | sc-365272 PE | 200 µg/ml | $349.00 | |||
VAC14 Antibody (F-12) Alexa Fluor® 488 | sc-365272 AF488 | 200 µg/ml | $364.00 | |||
VAC14 Antibody (F-12) Alexa Fluor® 546 | sc-365272 AF546 | 200 µg/ml | $364.00 | |||
VAC14 Antibody (F-12) Alexa Fluor® 594 | sc-365272 AF594 | 200 µg/ml | $364.00 | |||
VAC14 Antibody (F-12) Alexa Fluor® 647 | sc-365272 AF647 | 200 µg/ml | $364.00 | |||
VAC14 Antibody (F-12) Alexa Fluor® 680 | sc-365272 AF680 | 200 µg/ml | $364.00 | |||
VAC14 Antibody (F-12) Alexa Fluor® 790 | sc-365272 AF790 | 200 µg/ml | $364.00 |