V5-Probe Antibody (H-9) recommended for detection of nsr by WB, IP, IF and ELISA

V5-Probe Antibody (H-9): sc-271926

V5-Probe Antibody (H-9) is rated 3.6 out of 5 by 7.
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Datasheets

    • Anti-V5-Probe Antibody (H-9) is a mouse monoclonal IgG1 κ V5-Probe antibody, cited in 5 publications, provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 87-111 within a C-terminal sequence of the P and V proteins of Simian Virus 5 origin
    • V5-Probe Antibody (H-9) is recommended for detection of nsr by WB, IP, IF and ELISA
    • Anti-V5-Probe Antibody (H-9) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of V5-Probe (H-9): sc-271926.
    • m-IgG Fc BP-HRP (mouse IgG Fc binding protein-HRP) and m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP) are the preferred secondary detection reagents for V5-Probe Antibody (H-9) for WB applications. These reagents are now offered in bundles with V5-Probe Antibody (H-9) (see ordering information below).

At what molecular weight should the band be seen in Western Blot with V5-Probe (H-9): sc-271926 monoclonal antibody?

Asked by: jenni
Thank you for your question. The molecular weight expected varies depending on the fusion protein you are trying to detect, equal to the protein of interest plus the weight of the V5 tag that it is fused to.
Answered by: Technical Support 15
Date published: 2017-01-12

I am using the LiCor/Odyssey system. Which secondary antibody do you recommend using with V5-Probe (H-9): sc-271926?

Asked by: jenni
We recommend using one of our our exclusive Mouse IgG Binding Proteins (m-IgGκ) conjugated to CFL 680 or CFL 790. They are suitable for use with Near-Infrared (NIR) detection systems, such as LI-COR/Odyssey and other comparable systems. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 15
Date published: 2017-01-12
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Rated 2 out of 5 by from 没有明显带型 做WB,用的中间浓度,1/500稀释,化学发光显色,曝光曝了5min,没有看到明显的条带,效果一般。
Date published: 2018-03-16
Rated 1 out of 5 by from Poor I have tried transfected cells, however there are many non-specific binding bands with this antibody.
Date published: 2017-08-21
Rated 4 out of 5 by from Great antibody for western blotting and IP experim I used this antibody for pull down and detection of V5-tagged protein in mammalian cell lines by IP and western blotting. Works great.
Date published: 2017-08-11
Rated 4 out of 5 by from Strong western data Very clear western blot data; strong signal with low background
Date published: 2017-01-30
Rated 5 out of 5 by from Good V5 tag antibody Clear V5-tag detection by Western blot. Direct conjugates great to bypass using a secondary antibody.
Date published: 2017-01-25
Rated 4 out of 5 by from Good Antibody I could easily see if my U2OS cells were expressing my tagged protein in WB using this antibody.
Date published: 2016-12-05
Rated 5 out of 5 by from Good Western blot data of V5 Good Western blot data of V5-tagged fusion protein, non-tagged fusion protein, in non-transfected and V5-tagged human karyopherin 6 transfected 293T whole cell lysates. -SCBT QC
Date published: 2013-02-16
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