V5-Probe Antibody (H-9): sc-271926

V5-Probe Antibody (H-9) is a mouse monoclonal IgG1 kappa light chain antibody specifically engineered to recognize the V5 epitope, also known as the Pk or SV5 tag, which spans amino acids 87-111 within the C-terminal region of the P and V proteins from Simian Virus 5 (SV5). Anti-V5-Probe antibody (H-9) excels at detecting V5-tagged proteins across various applications, including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA), making V5-Probe (H-9) antibody a versatile tool for researchers working with fusion proteins. The V5 tag is frequently fused to proteins of interest to facilitate purification, visualization, and localization within different cellular compartments such as the cytoplasm, nucleus, or membrane-bound regions. Understanding the precise location of a protein within the cell is crucial for elucidating function and interactions, and V5-Probe monoclonal antibody (H-9) enables accurate detection and analysis in these contexts. By binding to the V5 epitope, anti-V5-Probe antibody (H-9) allows scientists to monitor protein expression levels, study protein-protein interactions, and investigate cellular pathways. Anti-V5-Probe antibody (H-9) can detect both transmembrane and secreted proteins, enhancing the study of diverse biological processes and disease mechanisms. Available in both non-conjugated and various conjugated forms, including horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates, V5-Probe (H-9) antibody offers flexibility to suit different experimental needs and detection methods.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-V5-Probe Antibody (H-9) has 8 citations in a variety of scientific publications.

Primary antibodies for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

V5-Probe Antibody (H-9) Specifications

Alternate Product Name: V5 tag

Product Clone Name: H-9

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 87-111

Epitope Target: the P and V proteins

Epitope Species: Simian Virus 5

Product Reactivity/Detection: V5 fusion proteins

Species Reactivity/Detection:

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 8 citations in various scientific publications

Available Conjugates/Forms for V5-Probe Antibody (H-9): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin

The preferred V5-Probe antibody is V5-Probe Antibody (E10): sc-81594.