V5-Probe Antibody (E10) recommended for detection of V5 fusion protein by WB, IP, IF and IHC(P)

V5-Probe Antibody (E10): sc-81594

V5-Probe Antibody (E10) is rated 4.3 out of 5 by 11.
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Datasheets

    • V5-Probe Antibody (E10) is a mouse monoclonal IgG1, cited in 21 publications, provided at 100 µg/ml
    • raised against V5 fusion protein
    • recommended for detection of V5 fusion protein by WB, IP, IF and IHC(P)
    • See V5-Probe (H-9): sc-271926 for V5-Probe antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of V5-Probe (E10): sc-81594.

At what molecular weight should the band be seen in Western Blot with this antibody?

Asked by: AbPolly
Thank you for your question. The molecular weight expected varies depending on the fusion protein you are trying to detect, equal to the protein of interest plus the weight of the V5 tag that it is fused to.
Answered by: Technical Support 8
Date published: 2019-06-06
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Rated 3 out of 5 by from Antibody review Worked with WB at 1:500 dilution with mosue tissue sample.
Date published: 2018-05-29
Rated 4 out of 5 by from good for western blot The V5-probe (E10) antibody works nicely for western blot. Havenot tried it in immunofluorescent staining.
Date published: 2018-01-26
Rated 5 out of 5 by from Good antibody for WB Detect V5-tagged protein in HeLa cells, used Ab in 1:1000, very strong and clean band.
Date published: 2018-01-26
Rated 4 out of 5 by from Clean antibody I received a sample from SCBT and have tested this antibody for western blotting. There is a non-specific band at ~150kDa. Other than that, it is a clean antibody.
Date published: 2017-10-23
Rated 5 out of 5 by from V5-CD40 fusion I have used this twice, and both times it was exceptionally clean, just a single strong band using chemiluminescence.
Date published: 2017-09-15
Rated 5 out of 5 by from clear signal the antibody was used of human, clear background.very good
Date published: 2017-05-02
Rated 5 out of 5 by from Great monoclonal antibody Specific signal observed with V5-Probe Antibody (E10)
Date published: 2017-01-18
Rated 5 out of 5 by from Superb Very clear western blot data; strong signal low background
Date published: 2017-01-02
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