V5-Probe Antibody (E10): sc-81594

V5-Probe Antibody (E10) is a mouse monoclonal IgG1 kappa light chain antibody that detects V5 fusion protein by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry with paraffin-embedded sections (IHCP). V5-Probe (E10) antibody is available in a non-conjugated form, specifically designed for detecting V5-tagged proteins commonly used in molecular biology for expression and purification of hybrid proteins. The V5 epitope, a small peptide derived from the Pk region of the paramyxovirus simian virus 5 (SV5), plays a crucial role in visualizing and purifying expressed fusion proteins. This small yet significant peptide allows researchers to track and isolate proteins of interest, facilitating studies on function and interactions. V5-Probe (E10) monoclonal antibody can tag over 20 recombinant proteins, including transmembrane and secreted proteins, enhancing experimental versatility. Using anti-V5 antibody (E10) in western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry with paraffin-embedded sections (IHCP) helps scientists gain valuable insights into protein expression, localization, and dynamics, making V5-Probe (E10) monoclonal antibody an essential tool in protein research.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

V5-Probe Antibody (E10) References:

1. Single amino acid substitution in the V protein of simian virus 5 differentiates its ability to block interferon signaling in human and murine cells.  |  Young, DF., et al. 2001. J Virol. 75: 3363-70. PMID: 11238862
2. The paramyxovirus SV5 V protein binds two atoms of zinc and is a structural component of virions.  |  Paterson, RG., et al. 1995. Virology. 208: 121-31. PMID: 11831692
3. A novel mechanism of repression of the vascular endothelial growth factor promoter, by single strand DNA binding cold shock domain (Y-box) proteins in normoxic fibroblasts.  |  Coles, LS., et al. 2002. Nucleic Acids Res. 30: 4845-54. PMID: 12433987
4. Paramyxoviruses SV5 and HPIV2 assemble STAT protein ubiquitin ligase complexes from cellular components.  |  Ulane, CM. and Horvath, CM. 2002. Virology. 304: 160-6. PMID: 12504558
5. Phosphorylation of cold shock domain/Y-box proteins by ERK2 and GSK3beta and repression of the human VEGF promoter.  |  Coles, LS., et al. 2005. FEBS Lett. 579: 5372-8. PMID: 16198352
6. A simian virus 5 (SV5) P/V mutant is less cytopathic than wild-type SV5 in human dendritic cells and is a more effective activator of dendritic cell maturation and function.  |  Arimilli, S., et al. 2006. J Virol. 80: 3416-27. PMID: 16537609
7. Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase.  |  Smith, DB. and Johnson, KS. 1988. Gene. 67: 31-40. PMID: 3047011
8. An Escherichia coli vector to express and purify foreign proteins by fusion to and separation from maltose-binding protein.  |  Maina, CV., et al. 1988. Gene. 74: 365-73. PMID: 3073105
9. Development of a V5-tag-directed nanobody and its implementation as an intracellular biosensor of GPCR signaling.  |  Zeghal, M., et al. 2023. J Biol Chem. 299: 105107. PMID: 37517699
10. Sequences of the malE gene and of its product, the maltose-binding protein of Escherichia coli K12.  |  Duplay, P., et al. 1984. J Biol Chem. 259: 10606-13. PMID: 6088507
11. Differential activation of the rat phenylethanolamine N-methyltransferase gene by Sp1 and Egr-1.  |  Ebert, SN. and Wong, DL. 1995. J Biol Chem. 270: 17299-305. PMID: 7615530
12. The RNA binding region of the paramyxovirus SV5 V and P proteins.  |  Lin, GY., et al. 1997. Virology. 238: 460-9. PMID: 9400618

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-V5-Probe Antibody (E10) has 34 citations in a variety of scientific publications.

Primary antibodies for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

V5-Probe Antibody (E10) Specifications

Alternate Product Name: V5 tag

Product Clone Name: E10

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: V5-Probe

Epitope Species:

Product Reactivity/Detection: V5 fusion proteins

Species Reactivity/Detection:

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 100 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 34 citations in various scientific publications

Available Conjugates/Forms for V5-Probe Antibody (E10): N/A

The preferred V5-Probe antibody is V5-Probe Antibody (H-9): sc-271926.