V-ATPase H Antibody (C-8): sc-166227
- V-ATPase H Antibody (C-8) is a mouse monoclonal IgG1 κ V-ATPase H antibody, cited in 8 publications, provided at 200 µg/ml
- raised against amino acids 184-483 mapping at the C-terminus of V-ATPase H of human origin
- V-ATPase H Antibody (C-8) is recommended for detection of V-ATPase H of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including and equine and canine
- Anti-V-ATPase H Antibody (C-8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for V-ATPase H Antibody (C-8) for WB and IHC(P) applications. These reagents are now offered in bundles with V-ATPase H Antibody (C-8) (see ordering information below).
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V-ATPase H Antibody (C-8) is a mouse monoclonal IgG1 kappa light chain antibody that detects V-ATPase H of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). V-ATPase H (C-8) antibody is available in non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The vacuolar-type H+-ATPase (V-ATPase) is a multisubunit enzyme that drives acidification of eukaryotic intracellular organelles, essential for protein sorting, nutrient uptake, and cellular signaling. V-ATPase pumps protons against an electrochemical gradient, maintaining pH homeostasis within organelles. V-ATPase includes a peripheral V1 domain for ATP hydrolysis and an integral V0 domain for proton translocation, with V1 comprising nine subunits (A-H) and V0 consisting of five subunits (a, d, c, c′, and c"). V-ATPase H subunit, also known as SDF, contains two polypeptides from the same gene and couples ATP hydrolysis to proton transport, ensuring efficient V-ATPase pump function.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
V-ATPase H Antibody (C-8) References:
- Subunit H of the V-ATPase binds to the medium chain of adaptor protein complex 2 and connects Nef to the endocytic machinery. | Geyer, M., et al. 2002. J Biol Chem. 277: 28521-9. PMID: 12032142
- Subunit H of the V-ATPase involved in endocytosis shows homology to beta-adaptins. | Geyer, M., et al. 2002. Mol Biol Cell. 13: 2045-56. PMID: 12058068
- Proton translocation driven by ATP hydrolysis in V-ATPases. | Kawasaki-Nishi, S., et al. 2003. FEBS Lett. 545: 76-85. PMID: 12788495
- Revised nomenclature for mammalian vacuolar-type H+ -ATPase subunit genes. | Smith, AN., et al. 2003. Mol Cell. 12: 801-3. PMID: 14580332
- Neurotransmitter release: the dark side of the vacuolar-H+ATPase. | Morel, N. 2003. Biol Cell. 95: 453-7. PMID: 14597263
- Human immunodeficiency virus Nef induces rapid internalization of the T-cell coreceptor CD8alphabeta. | Stove, V., et al. 2005. J Virol. 79: 11422-33. PMID: 16103193
- The vacuolar-ATPase B1 subunit in distal tubular acidosis: novel mutations and mechanisms for dysfunction. | Fuster, DG., et al. 2008. Kidney Int. 73: 1151-8. PMID: 18368028
- Vacuolar H+-ATPase subunit Vma1p functions as the molecular ligand in the vacuole-targeting fungicidal activity of polymyxin B. | Iida, M., et al. 2017. Microbiology (Reading). 163: 531-540. PMID: 28443813
- Endolysosomal impairment by binding of amyloid beta or MAPT/Tau to V-ATPase and rescue via the HYAL-CD44 axis in Alzheimer disease. | Kim, SH., et al. 2023. Autophagy. 19: 2318-2337. PMID: 36843263
- LAMTOR1 ubiquitination restricts its interaction with the vacuolar-type H+-ATPase, promotes autophagy and is controlled by USP32. | Hertel, A., et al. 2023. Autophagy. 19: 2406-2407. PMID: 36847417
- Glycolysis-Mediated Activation of v-ATPase by Nicotinamide Mononucleotide Ameliorates Lipid-Induced Cardiomyopathy by Repressing the CD36-TLR4 Axis. | Wang, S., et al. 2024. Circ Res. 134: 505-525. PMID: 38422177
- Interactions between HIV1 Nef and vacuolar ATPase facilitate the internalization of CD4. | Lu, X., et al. 1998. Immunity. 8: 647-56. PMID: 9620685
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
V-ATPase H Antibody (C-8) | sc-166227 | 200 µg/ml | $322.00 | |||
V-ATPase H Antibody (C-8): m-IgG Fc BP-HRP Bundle | sc-528926 | 200 µg Ab; 10 µg BP | $361.00 | |||
V-ATPase H Antibody (C-8): m-IgGκ BP-HRP Bundle | sc-521540 | 200 µg Ab, 40 µg BP | $361.00 | |||
V-ATPase H Antibody (C-8): m-IgG1 BP-HRP Bundle | sc-543216 | 200 µg Ab; 20 µg BP | $361.00 | |||
V-ATPase H Antibody (C-8) AC | sc-166227 AC | 500 µg/ml, 25% agarose | $424.00 | |||
V-ATPase H Antibody (C-8) HRP | sc-166227 HRP | 200 µg/ml | $322.00 | |||
V-ATPase H Antibody (C-8) FITC | sc-166227 FITC | 200 µg/ml | $336.00 | |||
V-ATPase H Antibody (C-8) PE | sc-166227 PE | 200 µg/ml | $349.00 | |||
V-ATPase H Antibody (C-8) Alexa Fluor® 488 | sc-166227 AF488 | 200 µg/ml | $364.00 | |||
V-ATPase H Antibody (C-8) Alexa Fluor® 546 | sc-166227 AF546 | 200 µg/ml | $364.00 | |||
V-ATPase H Antibody (C-8) Alexa Fluor® 594 | sc-166227 AF594 | 200 µg/ml | $364.00 | |||
V-ATPase H Antibody (C-8) Alexa Fluor® 647 | sc-166227 AF647 | 200 µg/ml | $364.00 | |||
V-ATPase H Antibody (C-8) Alexa Fluor® 680 | sc-166227 AF680 | 200 µg/ml | $364.00 | |||
V-ATPase H Antibody (C-8) Alexa Fluor® 790 | sc-166227 AF790 | 200 µg/ml | $364.00 |