V-ATPase G2 Antibody (A-6): sc-25334
- V-ATPase G2 Antibody (A-6) is a mouse monoclonal IgG2a κ V-ATPase G2 antibody, cited in 1 publications, provided at 200 µg/ml
- raised against amino acids 39-118 of V-ATPase G2 of human origin
- V-ATPase G2 Antibody (A-6) is recommended for detection of V-ATPase G2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-V-ATPase G2 Antibody (A-6) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for V-ATPase G2 Antibody (A-6) for WB and IHC(P) applications. These reagents are now offered in bundles with V-ATPase G2 Antibody (A-6) (see ordering information below).
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V-ATPase G2 Antibody (A-6) is a mouse monoclonal IgG2a kappa light chain antibody that detects V-ATPase G2 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). V-ATPase G2 (A-6) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. V-ATPase G2 plays a crucial role in acidifying eukaryotic intracellular organelles, which is essential for processes such as protein degradation, nutrient storage, and cellular pH regulation. V-ATPase G2 is located in organelle membranes like lysosomes and endosomes, where V-ATPase G2 facilitates proton transport across membranes, creating an acidic environment necessary for enzyme activation and proper cellular processes. The V-ATPase complex consists of two main domains: the peripheral V1 domain for ATP hydrolysis and the integral V0 domain for proton translocation. The G2 isoform interacts with the pore-forming a1C-subunit of L-type calcium channels, supporting proper membrane targeting and function. The genes encoding G1 and G2 V-ATPase subunits are located on chromosomes 9q33.1 and 6p21.3, respectively, highlighting their genetic significance in cellular physiology.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
V-ATPase G2 Antibody (A-6) References:
- A new member of the Ig superfamily and a V-ATPase G subunit are among the predicted products of novel genes close to the TNF locus in the human MHC. | Neville, MJ. and Campbell, RD. 1999. J Immunol. 162: 4745-54. PMID: 10202016
- Association of L-type calcium channels with a vacuolar H(+)-ATPase G2 subunit. | Gao, T. and Hosey, MM. 2000. Biochem Biophys Res Commun. 277: 611-6. PMID: 11062002
- The vacuolar (H+)-ATPases--nature's most versatile proton pumps. | Nishi, T. and Forgac, M. 2002. Nat Rev Mol Cell Biol. 3: 94-103. PMID: 11836511
- The intriguing evolution of the 'b' and 'G' subunits in F-type and V-type ATPases: isolation of the vma-10 gene from Neurospora crassa. | Hunt, IE. and Bowman, BJ. 1997. J Bioenerg Biomembr. 29: 533-40. PMID: 9559854
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
V-ATPase G2 Antibody (A-6) | sc-25334 | 200 µg/ml | $322.00 | |||
V-ATPase G2 Antibody (A-6): m-IgG Fc BP-HRP Bundle | sc-528407 | 200 µg Ab; 10 µg BP | $361.00 | |||
V-ATPase G2 Antibody (A-6): m-IgGκ BP-HRP Bundle | sc-520780 | 200 µg Ab, 40 µg BP | $361.00 | |||
V-ATPase G2 Antibody (A-6) AC | sc-25334 AC | 500 µg/ml, 25% agarose | $424.00 | |||
V-ATPase G2 Antibody (A-6) HRP | sc-25334 HRP | 200 µg/ml | $322.00 | |||
V-ATPase G2 Antibody (A-6) FITC | sc-25334 FITC | 200 µg/ml | $336.00 | |||
V-ATPase G2 Antibody (A-6) PE | sc-25334 PE | 200 µg/ml | $349.00 | |||
V-ATPase G2 Antibody (A-6) Alexa Fluor® 488 | sc-25334 AF488 | 200 µg/ml | $364.00 | |||
V-ATPase G2 Antibody (A-6) Alexa Fluor® 546 | sc-25334 AF546 | 200 µg/ml | $364.00 | |||
V-ATPase G2 Antibody (A-6) Alexa Fluor® 594 | sc-25334 AF594 | 200 µg/ml | $364.00 | |||
V-ATPase G2 Antibody (A-6) Alexa Fluor® 647 | sc-25334 AF647 | 200 µg/ml | $364.00 | |||
V-ATPase G2 Antibody (A-6) Alexa Fluor® 680 | sc-25334 AF680 | 200 µg/ml | $364.00 | |||
V-ATPase G2 Antibody (A-6) Alexa Fluor® 790 | sc-25334 AF790 | 200 µg/ml | $364.00 |