V-ATPase D Antibody (E-12): sc-390384

V-ATPase D Antibody (E-12) is a mouse monoclonal IgG1 kappa light chain antibody that detects V-ATPase D (V-ATPase D) of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). V-ATPase D (E-12) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The vacuolar-type H+-ATPase (V-ATPase) is a crucial multisubunit enzyme that plays a vital role in acidifying eukaryotic intracellular organelles, essential for processes such as protein degradation, nutrient storage, and cellular signaling. V-ATPase functions by pumping protons against an electrochemical gradient, maintaining pH homeostasis within organelles and facilitating proper cellular processes. V-ATPase consists of a peripheral V1 domain responsible for ATP hydrolysis and an integral V0 domain responsible for proton translocation, with V1 domain comprising nine subunits (A-H) and V0 domain containing five subunits (a, d, c, c′, and c"). V-ATPase C, an auxiliary subunit, exhibits ubiquitous expression, highlighting V-ATPase′s importance across different cell types. Anti-V-ATPase D antibody (E-12) serves as an invaluable tool for researchers studying intracellular pH regulation and V-ATPase′s role in various physiological and pathological contexts.

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V-ATPase D Antibody (E-12) References:

1. Gene expression profiling in the human hypothalamus-pituitary-adrenal axis and full-length cDNA cloning.  |  Hu, RM., et al. 2000. Proc Natl Acad Sci U S A. 97: 9543-8. PMID: 10931946
2. The vacuolar (H+)-ATPases--nature's most versatile proton pumps.  |  Nishi, T. and Forgac, M. 2002. Nat Rev Mol Cell Biol. 3: 94-103. PMID: 11836511
3. Atp6v0d2 is an essential component of the osteoclast-specific proton pump that mediates extracellular acidification in bone resorption.  |  Wu, H., et al. 2009. J Bone Miner Res. 24: 871-85. PMID: 19113919
4. Molecular cloning of cDNA encoding the C subunit of H(+)-ATPase from bovine chromaffin granules.  |  Nelson, H., et al. 1990. J Biol Chem. 265: 20390-3. PMID: 2147024
5. Changes in gene expression in the rat hippocampus after focal cerebral ischemia.  |  Chung, JY., et al. 2011. J Korean Neurosurg Soc. 50: 173-8. PMID: 22102944
6. High bone mass in mice lacking Cx37 because of defective osteoclast differentiation.  |  Pacheco-Costa, R., et al. 2014. J Biol Chem. 289: 8508-20. PMID: 24509854
7. Acidification of uterine epithelium during embryo implantation in mice.  |  Xiao, S., et al. 2017. Biol Reprod. 96: 232-243. PMID: 28395338
8. Co-expression network analysis identifies a gene signature as a predictive biomarker for energy metabolism in osteosarcoma.  |  Zhu, N., et al. 2020. Cancer Cell Int. 20: 259. PMID: 32581649
9. The SARS-CoV-2 Cytopathic Effect Is Blocked by Lysosome Alkalizing Small Molecules.  |  Gorshkov, K., et al. 2021. ACS Infect Dis. 7: 1389-1408. PMID: 33346633
10. Unfolded Protein Response-Related Signature Associates With the Immune Microenvironment and Prognostic Prediction in Osteosarcoma.  |  Zhang, Z., et al. 2022. Front Genet. 13: 911346. PMID: 35754801
11. [Artificial light at night effects glucose metabolism in the developing jawbone by inhibiting melatonin secretion].  |  Tang, QM., et al. 2025. Zhonghua Kou Qiang Yi Xue Za Zhi. 60: 140-150. PMID: 39848802
12. Cloning and tissue distribution of subunits C, D, and E of the human vacuolar H(+)-ATPase.  |  van Hille, B., et al. 1993. Biochem Biophys Res Commun. 197: 15-21. PMID: 8250920

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-V-ATPase D Antibody (E-12) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-V-ATPase D Antibody (E-12) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

V-ATPase D Antibody (E-12) Specifications

Alternate Product Name: V-ATPase D

Product Clone Name: E-12

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 211-249

Epitope Target: V-ATPase D

Epitope Species: human

Product Reactivity/Detection: V-ATPase D

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for V-ATPase D Antibody (E-12): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin