Date published: 2026-5-5
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V-ATPase D Antibody (A-11) - Western Blotting - Image 375448
V-ATPase D Antibody (A-11): sc-390164. Western blot analysis of V-ATPase D expression in SK-N-SH (A), U-87 MG (B), TF-1 (C), NIH/3T3 (D), EOC 20 (E) and C6 (F) whole cell lysates.
V-ATPase D Antibody (A-11) - Western Blotting - Image 375448
V-ATPase D Antibody (A-11): sc-390164. Western blot analysis of V-ATPase D expression in SK-N-SH (A), U-87 MG (B), TF-1 (C), NIH/3T3 (D), EOC 20 (E) and C6 (F) whole cell lysates.
V-ATPase D Antibody (A-11) - Western Blotting - Image 287213
V-ATPase D Antibody (A-11): sc-390164. Western blot analysis of V-ATPase D expression in SK-N-SH whole cell lysate (A) and mouse brain (B) and human hippocamus (C) tissue extracts.
V-ATPase D Antibody (A-11) - Western Blotting - Image 287906
V-ATPase D Antibody (A-11): sc-390164. Western blot analysis of V-ATPase D expression in rat brain tissue extract.
V-ATPase D Antibody (A-11): sc-390164. Western blot analysis of V-ATPase D expression in SK-N-SH (A), U-87 MG (B), TF-1 (C), NIH/3T3 (D), EOC 20 (E) and C6 (F) whole cell lysates.

V-ATPase D Antibody (A-11): sc-390164

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Datasheets
  • V-ATPase D Antibody (A-11) is a mouse monoclonal IgG2b κ, cited in 1 publications, provided at 200 µg/ml
  • raised against a peptide mapping near the C-terminus of V-ATPase D of human origin
  • recommended for detection of V-ATPase D of mouse, rat and human origin by WB, IP, IF and ELISA
  • m-IgG2b BP-HRP is the preferred secondary detection reagent for V-ATPase D Antibody (A-11) for WB applications. This reagent is now offered in a bundle with V-ATPase D Antibody (A-11) (see ordering information below).
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Additional V-ATPase Antibodies including V-ATPase A1, V-ATPase α 1, V-ATPase B1, V-ATPase B2, V-ATPase C1, V-ATPase C2, V-ATPase D, V-ATPase D1, V-ATPase D2, V-ATPase G1, V-ATPase G2 and V-ATPase H

V-ATPase D Antibody (A-11) is a mouse monoclonal IgG2b antibody that detects V-ATPase D in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). V-ATPase D is a crucial component of the vacuolar-type H+-ATPase complex, which plays a vital role in the acidification of intracellular organelles, thereby influencing various cellular processes such as protein sorting, nutrient storage, and cellular signaling. The V-ATPase complex is composed of two main domains: the peripheral V1 domain, responsible for ATP hydrolysis, and the integral V0 domain, which facilitates proton translocation across membranes. V-ATPase D (A-11) antibody targets a subunit of the V0 domain that is essential for proper assembly and function of the enzyme, and V-ATPase D expression is ubiquitous across different tissues, highlighting its importance in maintaining cellular homeostasis. Anti-V-ATPase D antibody (A-11) enables researchers to effectively study the dynamics of V-ATPase D in various biological contexts, providing insights into its role in health and disease.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA

LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences

V-ATPase D Antibody (A-11) References:

  1. Distinct expression patterns of different subunit isoforms of the V-ATPase in the rat epididymis.  |  Pietrement, C., et al. 2006. Biol Reprod. 74: 185-94. PMID: 16192400
  2. Control elements in the neighboring ATPase gene influence spatiotemporal expression of the human agouti-related protein.  |  Ilnytska, O., et al. 2009. J Mol Biol. 388: 239-51. PMID: 19285986
  3. HPS6 Regulates the Biogenesis of Weibel-Palade Body in Endothelial Cells Through Trafficking v-ATPase to Its Limiting Membrane.  |  Lu, J., et al. 2021. Front Cell Dev Biol. 9: 743124. PMID: 35252216
  4. A Novel Somatic Mutation Implicates ATP6V0D1 in Proinsulin Processing.  |  Avari, P., et al. 2023. J Endocr Soc. 7: bvac196. PMID: 36694809
  5. Identification of Novel Senescent Markers in Small Extracellular Vesicles.  |  Misawa, T., et al. 2023. Int J Mol Sci. 24: PMID: 36768745
  6. Transcriptome analysis of skeletal muscle in dermatomyositis, polymyositis, and dysferlinopathy, using a bioinformatics approach.  |  Jeong, HN., et al. 2023. Front Neurol. 14: 1328547. PMID: 38125829
  7. Integrating iron metabolism-related gene signature to evaluate prognosis and immune infiltration in nasopharyngeal carcinoma.  |  Su, J., et al. 2024. Discov Oncol. 15: 112. PMID: 38602575
  8. Integrated analysis of single-cell and bulk RNA sequencing data reveals prognostic characteristics of lysosome-dependent cell death-related genes in osteosarcoma.  |  Wu, Y., et al. 2024. BMC Genomics. 25: 379. PMID: 38632516
  9. Alkaliptosis induction counteracts paclitaxel-resistant ovarian cancer cells via ATP6V0D1-mediated ABCB1 inhibition.  |  Chen, F., et al. 2024. Mol Carcinog. 63: 1515-1527. PMID: 38751020
  10. Physical Exercise Decreases Complement-Mediated Synaptic Loss and Protects Against Cognitive Impairment by Inhibiting Microglial Tmem9-ATP6V0D1 in Alzheimer's Disease.  |  Li, S., et al. 2025. Aging Cell. e14496. PMID: 39871402

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

V-ATPase D Antibody (A-11)

sc-390164
200 µg/ml
$322.00

V-ATPase D Antibody (A-11): m-IgG2b BP-HRP Bundle

sc-548617
200 µg Ab; 10 µg BP
$361.00

V-ATPase D (A-11) Neutralizing Peptide

sc-390164 P
100 µg/0.5 ml
$69.00

What application is the blocking peptide sc-390164 P appropriate for?

Asked by: TinTin
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-03-01
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Rated 5 out of 5 by from Nice Western Blot data of VNice Western Blot data of V-ATPase D expression in rat brain tissue extract. -SCBT QC
Date published: 2015-03-10
Rated 5 out of 5 by from Good Western Blot data of VGood Western Blot data of V-ATPase D expression in SK-N-SH whole cell lysate and mouse brain and human hippocamus tissue extracts. -SCBT QC
Date published: 2014-10-20
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V-ATPase D Antibody (A-11) is rated 5.0 out of 5 by 2.
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