Anti-V-ATPase C1 Antibody (G-5) recommended for detection of V-ATPase C1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

Anti-V-ATPase C1 Antibody (G-5): sc-271077

Anti-V-ATPase C1 Antibody (G-5) is rated 4.6 out of 5 by 5.
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Datasheets

    • Anti-V-ATPase C1 Antibody (G-5) is a mouse monoclonal IgG2b κ V-ATPase C1 antibody, cited in 4 publications, provided at 200 µg/ml
    • raised against amino acids 83-382 of V-ATPase C1 of human origin
    • Anti-V-ATPase C1 Antibody (G-5) is recommended for detection of V-ATPase C1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-V-ATPase C1 Antibody (G-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of V-ATPase C1 (G-5): sc-271077.
    • m-IgGκ BP-HRP is the preferred secondary detection reagent for V-ATPase C1 Antibody (G-5) for WB and IHC(P) applications. This reagent is now offered in a bundle with V-ATPase C1 Antibody (G-5) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.
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I am using V-ATPase C1 (G-5): sc-271077 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: Germaine
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 3 out of 5 by from 免疫沈降で使用 Immunoprecipitationで使用。5ul/sampleで用いた。IPできているものの、あまり強くはない。
Date published: 2020-06-19
Rated 5 out of 5 by from Excellent immunoperoxidase cytoplasmic staining Excellent immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human esophagus tissue. -SCBT QC
Date published: 2015-04-29
Rated 5 out of 5 by from Endogenous levels of this protein detected Endogenous levels of this protein detected using product sc-271077 by WB on 293T (non-transfected), HeLa and HepG2 whole cell lysates. -SCBT QC
Date published: 2015-04-02
Rated 5 out of 5 by from Good specificity shown by using transfected Good specificity shown by using transfected and non-transfected 293T cells for over-expression of the mouse V-ATPase C1 protein via Western Blot analysis. -SCBT QC
Date published: 2015-03-06
Rated 5 out of 5 by from Positive detection of endogenous expression of V Positive detection of endogenous expression of V-ATPase C1 in SK-N-MC whole cell lysate, mouse kidney tissue extract and rat brain tissue extract. -SCBT QC
Date published: 2015-01-14
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