V-ATPase C1 Antibody (G-5): sc-271077

V-ATPase C1 Antibody (G-5) is a mouse monoclonal IgG2b kappa light chain antibody that detects V-ATPase C1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Anti-V-ATPase C1 antibody (G-5) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The vacuolar-type H+-ATPase (V-ATPase) is a crucial multisubunit enzyme that plays a vital role in acidifying eukaryotic intracellular organelles, essential for processes such as protein sorting, nutrient uptake, and cellular signaling. V-ATPase pumps protons against an electrochemical gradient, a mechanism fundamental for maintaining pH homeostasis within cells. V-ATPase consists of a peripheral V1 domain responsible for ATP hydrolysis and an integral V0 domain that facilitates proton translocation, with V1 domain consisting of nine subunits (A-H) and V0 domain comprising five subunits (a, d, c, c′, and c"). V-ATPase C serves as an auxiliary subunit with widespread expression, and human V-ATPase C gene is located on chromosome 8q22.3. Understanding V-ATPase C1 structure and function is critical, as V-ATPase C1 contributes to enzyme activity and plays a role in regulating various physiological processes, making V-ATPase C1 a significant target for research in cellular biology and potential therapeutic interventions.

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V-ATPase C1 Antibody (G-5) References:

1. Gene expression profiling in the human hypothalamus-pituitary-adrenal axis and full-length cDNA cloning.  |  Hu, RM., et al. 2000. Proc Natl Acad Sci U S A. 97: 9543-8. PMID: 10931946
2. The vacuolar (H+)-ATPases--nature's most versatile proton pumps.  |  Nishi, T. and Forgac, M. 2002. Nat Rev Mol Cell Biol. 3: 94-103. PMID: 11836511
3. Intracellular pH regulation in oral squamous cell carcinoma is mediated by increased V-ATPase activity via over-expression of the ATP6V1C1 gene.  |  Otero-Rey, EM., et al. 2008. Oral Oncol. 44: 193-9. PMID: 17467328
4. Atp6v1c1 is an essential component of the osteoclast proton pump and in F-actin ring formation in osteoclasts.  |  Feng, S., et al. 2009. Biochem J. 417: 195-203. PMID: 18657050
5. Measurement of ATP6V1C1 expression in brush cytology samples as a diagnostic and prognostic marker in oral squamous cell carcinoma.  |  Pérez-Sayáns, M., et al. 2010. Cancer Biol Ther. 9: 1057-64. PMID: 20404513
6. Molecular cloning of cDNA encoding the C subunit of H(+)-ATPase from bovine chromaffin granules.  |  Nelson, H., et al. 1990. J Biol Chem. 265: 20390-3. PMID: 2147024
7. Targeting Atp6v1c1 Prevents Inflammation and Bone Erosion Caused by Periodontitis and Reveals Its Critical Function in Osteoimmunology.  |  Li, S., et al. 2015. PLoS One. 10: e0134903. PMID: 26274612
8. Expression of ATP6V1C1 during oral carcinogenesis.  |  Oliveira Alves, MG., et al. 2016. Biotech Histochem. 91: 263-8. PMID: 26984774
9. MicroRNA29a Reverts the Activated Hepatic Stellate Cells in the Regression of Hepatic Fibrosis through Regulation of ATPase H⁺ Transporting V1 Subunit C1.  |  Jing, F., et al. 2019. Int J Mol Sci. 20: PMID: 30781750
10. The ATPase subunit of ATP6V1C1 inhibits autophagy and enhances radiotherapy resistance in esophageal squamous cell carcinoma.  |  Yao, X., et al. 2021. Gene. 768: 145261. PMID: 33183740
11. ATP6V1C1, associated with the tumor microenvironment and mTORC1 signaling pathway, is a potential diagnostic, prognostic, and therapeutic biomarker for hepatocellular carcinoma.  |  Pan, Y., et al. 2024. Discov Oncol. 15: 673. PMID: 39557733
12. Cloning and tissue distribution of subunits C, D, and E of the human vacuolar H(+)-ATPase.  |  van Hille, B., et al. 1993. Biochem Biophys Res Commun. 197: 15-21. PMID: 8250920

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-V-ATPase C1 Antibody (G-5) has 4 citations in a variety of scientific publications.

Primary antibodies like Anti-V-ATPase C1 Antibody (G-5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

V-ATPase C1 Antibody (G-5) Specifications

Alternate Product Name: V-ATPase C1

Product Clone Name: G-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 83-382

Epitope Target: V-ATPase C1

Epitope Species: human

Product Reactivity/Detection: V-ATPase C1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 4 citations in various scientific publications

Available Conjugates/Forms for V-ATPase C1 Antibody (G-5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin