V-ATPase B2 Antibody (D-11): sc-166045

V-ATPase B2 Antibody (D-11) is a mouse monoclonal IgG2a kappa light chain antibody that detects V-ATPase B2 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Anti-V-ATPase B2 antibody (D-11) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The vacuolar-type H+-ATPase (V-ATPase) is a crucial multisubunit enzyme that plays a vital role in the acidification of eukaryotic intracellular organelles, which is essential for processes such as protein degradation, nutrient absorption, and maintaining cellular homeostasis. The V-ATPase complex consists of a peripheral V1 domain responsible for ATP hydrolysis and an integral V0 domain that facilitates proton translocation, with the V1 domain comprising nine subunits (A-H) and the V0 domain consisting of five subunits (a, d, c, c′, and c"). Notably, V-ATPase B2 is predominantly expressed in the kidney and osteoclasts, where V-ATPase B2 is involved in bone resorption and maintaining the acidic environment necessary for the dissolution of bone minerals. The gene encoding V-ATPase B2 is located on chromosome 8p22-p21, and understanding V-ATPase B2 function and interactions is critical for elucidating V-ATPase B2′s role in renal physiology and osteoclast activity.

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V-ATPase B2 Antibody (D-11) References:

1. The vacuolar (H+)-ATPases--nature's most versatile proton pumps.  |  Nishi, T. and Forgac, M. 2002. Nat Rev Mol Cell Biol. 3: 94-103. PMID: 11836511
2. Selectively amplified expression of an isoform of the vacuolar H(+)-ATPase 56-kilodalton subunit in renal intercalated cells.  |  Nelson, RD., et al. 1992. Proc Natl Acad Sci U S A. 89: 3541-5. PMID: 1373501
3. Inhibition of osteoclast bone resorption by disrupting vacuolar H+-ATPase a3-B2 subunit interaction.  |  Kartner, N., et al. 2010. J Biol Chem. 285: 37476-90. PMID: 20837476
4. An mRNA from human brain encodes an isoform of the B subunit of the vacuolar H(+)-ATPase.  |  Bernasconi, P., et al. 1990. J Biol Chem. 265: 17428-31. PMID: 2145275
5. Interaction between V-ATPase B2 and (Pro) renin Receptors in Promoting the progression of Renal Tubulointerstitial Fibrosis.  |  Liu, Y., et al. 2016. Sci Rep. 6: 25035. PMID: 27121029
6. Corrigendum: Interaction between V-ATPase B2 and (Pro) renin Receptors in Promoting the progression of Renal Tubulointerstitial Fibrosis.  |  Liu, Y., et al. 2016. Sci Rep. 6: 27677. PMID: 27282103
7. Impaired V-ATPase leads to increased lysosomal pH, results in disrupted lysosomal degradation and autophagic flux blockage, contributes to fluoride-induced developmental neurotoxicity.  |  Han, X., et al. 2022. Ecotoxicol Environ Saf. 236: 113500. PMID: 35421827
8. Overexpression of V-ATPase B2 attenuates lung injury/fibrosis by stabilizing lysosomal membrane permeabilization and increasing collagen degradation.  |  Lee, JU., et al. 2022. Exp Mol Med. 54: 662-672. PMID: 35624153
9. V-ATPase B2 promotes microglial phagocytosis of myelin debris by inactivating the MAPK signaling pathway.  |  Li, Y., et al. 2024. Neuropeptides. 106: 102436. PMID: 38733728
10. Osteoclasts express the B2 isoform of vacuolar H(+)-ATPase intracellularly and on their plasma membranes.  |  Lee, BS., et al. 1996. Am J Physiol. 270: C382-8. PMID: 8772466
11. Mutations in the gene encoding B1 subunit of H+-ATPase cause renal tubular acidosis with sensorineural deafness.  |  Karet, FE., et al. 1999. Nat Genet. 21: 84-90. PMID: 9916796

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-V-ATPase B2 Antibody (D-11) has 4 citations in a variety of scientific publications.

Primary antibodies like Anti-V-ATPase B2 Antibody (D-11) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

V-ATPase B2 Antibody (D-11) Specifications

Alternate Product Name: ATP6V1B2

Product Clone Name: D-11

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 2-38

Epitope Target: V-ATPase B2

Epitope Species: human

Product Reactivity/Detection: V-ATPase B2

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: canine and bovine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 4 citations in various scientific publications

Available Conjugates/Forms for V-ATPase B2 Antibody (D-11): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin

The preferred V-ATPase B1/2 antibody is V-ATPase B1/2 Antibody (F-6): sc-55544.