USP9 Antibody (5G-02): sc-100628

USP9 Antibody (5G-02) is a mouse monoclonal IgG1 kappa light chain antibody that detects USP9 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-USP9 antibody (5G-02) is available as a non-conjugated monoclonal isotype antibody. USP9 protein, also known as ubiquitin specific peptidase 9 or USP9X, plays a crucial role in the ubiquitin-proteasome pathway, which is essential for regulating various cellular processes, including signal transduction, transcriptional activation, and cell cycle progression. USP9 is located in the cytoplasm and nucleus, where USP9 is involved in the deubiquitination of target proteins, thus preventing their degradation and maintaining cellular homeostasis. USP9′s ability to interact with a variety of substrates, including those involved in cell cycle regulation and apoptosis, underscores USP9′s importance in cellular function and development. Notably, defects in USP9 have been linked to Turner syndrome, a genetic disorder that affects female development and is characterized by the failure of oocyte proliferation and ovarian degeneration. With a length of 2547 amino acids, USP9 is a member of the peptidase C19 family of ubiquitin proteases and is expressed in both fetal and adult tissues, highlighting USP9′s fundamental role in protein turnover and cellular regulation. Multiple isoforms of USP9 arise from alternative splicing, further emphasizing the complexity and regulatory potential of USP9 in various biological contexts.

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USP9 Antibody (5G-02) References:

1. Usp9y (ubiquitin-specific protease 9 gene on the Y) is associated with a functional promoter and encodes an intact open reading frame homologous to Usp9x that is under selective constraint.  |  Hall, NM., et al. 2003. Mamm Genome. 14: 437-47. PMID: 12925892
2. Doublecortin interacts with the ubiquitin protease DFFRX, which associates with microtubules in neuronal processes.  |  Friocourt, G., et al. 2005. Mol Cell Neurosci. 28: 153-64. PMID: 15607950
3. Spatially and temporally specific expression in mouse hippocampus of Usp9x, a ubiquitin-specific protease involved in synaptic development.  |  Xu, J., et al. 2005. J Neurosci Res. 80: 47-55. PMID: 15723417
4. Age-related changes in Usp9x protein expression and DNA methylation in mouse brain.  |  Xu, J. 2005. Brain Res Mol Brain Res. 140: 17-24. PMID: 16023255
5. 14-3-3 cooperates with LKB1 to regulate the activity and localization of QSK and SIK.  |  Al-Hakim, AK., et al. 2005. J Cell Sci. 118: 5661-73. PMID: 16306228
6. The ubiquitin ligase itch is auto-ubiquitylated in vivo and in vitro but is protected from degradation by interacting with the deubiquitylating enzyme FAM/USP9X.  |  Mouchantaf, R., et al. 2006. J Biol Chem. 281: 38738-47. PMID: 17038327
7. Control of AMPK-related kinases by USP9X and atypical Lys(29)/Lys(33)-linked polyubiquitin chains.  |  Al-Hakim, AK., et al. 2008. Biochem J. 411: 249-60. PMID: 18254724
8. Characterisation of the coding sequence and fine mapping of the human DFFRY gene and comparative expression analysis and mapping to the Sxrb interval of the mouse Y chromosome of the Dffry gene.  |  Brown, GM., et al. 1998. Hum Mol Genet. 7: 97-107. PMID: 9384609

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-USP9 Antibody (5G-02) has 5 citations in a variety of scientific publications.

Primary antibodies like Anti-USP9 Antibody (5G-02) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

USP9 Antibody (5G-02) Specifications

Alternate Product Name: USP9

Product Clone Name: 5G-02

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-91

Epitope Target: USP9

Epitope Species: human

Product Reactivity/Detection: USP9

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 100 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 5 citations in various scientific publications

Available Conjugates/Forms for USP9 Antibody (5G-02): N/A