Date published: 2025-9-5

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USP6/32 抗体 (D-11): sc-377306

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说明书
  • USP6/32 抗体 D-11 是小鼠单克隆 IgG1 κ,USP6/32抗体, 在2篇文献中引用,规格为200 µg/ml
  • 免疫原氨基酸序列1117-1406位于human物种的USP6的C-terminus
  • USP6/32 抗体 (D-11) 推荐用于 WB, IP, IF 和 ELISA,检测 USP6 isoforms 1 and 2, and USP32 of human origin, and USP32 of mouse and rat origin
  • 抗USP6/32抗体(D-11)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取USP6/32 (D-11): sc-377306免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgGκ BP-HRP是USP6/32 Antibody (D-11) 适用于 WB 应用。 的首选辅助检测试剂。该试剂现可与USP6/32 Antibody (D-11) 搭配使用(请参阅下面的订购信息)。有关其它 m-IgGκ BP 结合物,请参阅我们完整的小鼠 IgG 结合蛋白列表。

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関連項目

USP6/32抗体(D-11)是一种IgG1κ小鼠单克隆USP6/32抗体,可通过WB、IP、IF和ELISA检测小鼠、大鼠和人类的USP6/32蛋白。USP6/32抗体(D-11)既以非共轭抗USP6/32抗体形式提供,也以多种共轭形式的抗USP6/32抗体形式提供,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®共轭物。泛素特异性蛋白酶(USP6)是一种含有1406个氨基酸的蛋白质,具有极强的亲水性,并有两个电荷簇,这是核酸结合区域的特征。USP6基因具有致癌性,起源于两个基因的嵌合融合:USP32和TBC1D3。USP32是一个古老且高度保守的基因,而TBC1D3基因则来源于相对较近的片段重复。这种重复在大多数其他哺乳动物中是不存在的,并且在整个灵长类谱系中显示出快速扩增和传播。然而,嵌合的USP6仅存在于灵长类动物的人科谱系中,因此它可能对人科物种的形成有所贡献。USP6是睾丸特异性的,这暗示了它可能与生殖屏障的出现有关。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

USP6/32 抗体 (D-11) 参考文献:

  1. 编码 TBC1D1 的基因与 tre-2/USP6 肿瘤基因, BUB2 和 cdc16 同源,位于小鼠 5 号染色体和人类 4 号染色体上。  |  White, RA., et al. 2000. Cytogenet Cell Genet. 89: 272-5. PMID: 10965142
  2. Tre2(USP6)癌基因是一种同源染色体特异性基因。  |  Paulding, CA., et al. 2003. Proc Natl Acad Sci U S A. 100: 2507-11. PMID: 12604796
  3. 动脉瘤性骨囊肿中的 USP6 (Tre2) 融合癌基因。  |  Oliveira, AM., et al. 2004. Cancer Res. 64: 1920-3. PMID: 15026324
  4. USP6 和 CDH11 致癌基因可识别原发性动脉瘤性骨囊肿中的肿瘤细胞,而在所谓的继发性动脉瘤性骨囊肿中则不存在。  |  Oliveira, AM., et al. 2004. Am J Pathol. 165: 1773-80. PMID: 15509545
  5. 动脉瘤性骨囊肿变异易位通过与 ZNF9, COL1A1, TRAP150 和 OMD 基因的启动子交换上调 USP6 的转录。  |  Oliveira, AM., et al. 2005. Oncogene. 24: 3419-26. PMID: 15735689
  6. 钙/钙调蛋白调节泛素特异性蛋白酶 TRE17/USP6 的泛素化。  |  Shen, C., et al. 2005. J Biol Chem. 280: 35967-73. PMID: 16127172
  7. 动脉瘤性骨囊肿:一种由 USP6 癌基因上调驱动的肿瘤。  |  Oliveira, AM., et al. 2006. J Clin Oncol. 24: e1; author reply e2. PMID: 16382110
  8. 两个染色体易位家族中TRE-2(USP6)癌基因, 低拷贝重复和异中心异染色质的参与证据。  |  Ou, Z., et al. 2006. Hum Genet. 120: 227-37. PMID: 16791615
  9. 肌腱鞘细胞纤维瘤中的USP6基因重排。  |  Carter, JM., et al. 2016. Mod Pathol. 29: 865-9. PMID: 27125357
  10. 乳腺结节性筋膜炎:临床病理和分子特征,并鉴定新型USP6融合伴侣。  |  Cloutier, JM., et al. 2021. Mod Pathol. 34: 1865-1875. PMID: 34099872
  11. [肌腱鞘纤维瘤:134例的临床病理和遗传分析]。  |  Cui, J., et al. 2023. Zhonghua Bing Li Xue Za Zhi. 52: 364-369. PMID: 36973197
  12. 掌结节性筋膜炎携带新型SREBF1::USP6融合基因。  |  Mejbel, HA., et al. 2024. Int J Surg Pathol. 32: 1494-1499. PMID: 38304953

订购信息

产品名称产品编号规格价格数量收藏夹

USP6/32 抗体 (D-11)

sc-377306
200 µg/ml
$316.00

USP6/32 (D-11): m-IgGκ BP-HRP 套装

sc-523275
200 µg Ab, 40 µg BP
$354.00

USP6/32 抗体 (D-11) AC

sc-377306 AC
500 µg/ml, 25% agarose
$416.00

USP6/32 抗体 (D-11) HRP

sc-377306 HRP
200 µg/ml
$316.00

USP6/32 抗体 (D-11) FITC

sc-377306 FITC
200 µg/ml
$330.00

USP6/32 抗体 (D-11) PE

sc-377306 PE
200 µg/ml
$343.00

USP6/32 抗体 (D-11) Alexa Fluor® 488

sc-377306 AF488
200 µg/ml
$357.00

USP6/32 抗体 (D-11) Alexa Fluor® 546

sc-377306 AF546
200 µg/ml
$357.00

USP6/32 抗体 (D-11) Alexa Fluor® 594

sc-377306 AF594
200 µg/ml
$357.00

USP6/32 抗体 (D-11) Alexa Fluor® 647

sc-377306 AF647
200 µg/ml
$357.00

USP6/32 抗体 (D-11) Alexa Fluor® 680

sc-377306 AF680
200 µg/ml
$357.00

USP6/32 抗体 (D-11) Alexa Fluor® 790

sc-377306 AF790
200 µg/ml
$357.00

Application of USP6/32 (D-11): SC-377306 monoclonal antibody to IHC-FFPE is possible? If possible, what is the recommended method?

Asked by: SakuraMoon
Thank you for your question.USP6/32 Antibody (D-11) is recommended for detection of USP6 isoforms 1 and 2, and USP32 of human origin, and USP32 of mouse and rat origin by WB, IP, IF and ELISA.If it is not indicated that it is recommended for IHC (P), it will not support immunofluorescence or immunohistochemical testing of paraffin sections. The antibody can be used for immunoassay on frozen sections or cell samples. thank you
Answered by: Jiajun Z
Date published: 2023-03-27

What is the recommended fixation method for immunofluorescence staining with USP6/32 (D-11): sc-377306 monoclonal antibody?

Asked by: AbPolly
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Produced beautiful immunofluorescence GolgiProduced beautiful immunofluorescence Golgi apparatus and cytoplasmic staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-01-09
Rated 5 out of 5 by from Produced positive Western Blot data of USP6/32Produced positive Western Blot data of USP6/32 expression in Caki-1 whole cell lysate and human testis tissue extract . -SCBT QC
Date published: 2013-07-04
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USP6/32 抗体 (D-11) is rated 5.0 out of 5 by 2.
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