USP32 Antibody (A-10): sc-374465
- USP32 Antibody (A-10) is a mouse monoclonal IgG2b κ, cited in 9 publications, provided at 200 µg/ml
- raised against amino acids 361-660 mapping within an internal region of USP32 of human origin
- Anti-USP32 Antibody (A-10) is recommended for detection of USP32 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-USP32 Antibody (A-10) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for USP32 Antibody (A-10) for WB applications. These reagents are now offered in bundles with USP32 Antibody (A-10) (see ordering information below).
USP32 Antibody (A-10) is a mouse monoclonal IgG2b kappa light chain antibody that detects USP32 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). USP32 (A-10) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. USP32, also known as ubiquitin specific peptidase 32 or NY-REN-60, is a 1,604 amino acid protein characterized by one dual specificity phosphatase domain and three EF-hand calcium binding domains, which are crucial for enzymatic activity. USP32 is primarily localized to cellular membranes in a lipid-anchored manner and is expressed across all normal tissues, highlighting a fundamental role in cellular homeostasis. USP32 plays a vital role in the ubiquitin-proteasome pathway, catalyzing the conversion of a ubiquitin C-terminal thioester to free ubiquitin and a thiol, a process essential for regulating various cellular functions, including signal transduction, transcriptional activation, and cell cycle progression. USP32′s ability to modulate the ubiquitination status of target proteins underscores an importance in maintaining cellular integrity and responding to stress signals, making USP32 a significant focus of research in understanding disease mechanisms and potential therapeutic interventions.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
USP32 Antibody (A-10) References:
- Antigens recognized by autologous antibody in patients with renal-cell carcinoma. | Scanlan, MJ., et al. 1999. Int J Cancer. 83: 456-64. PMID: 10508479
- The Tre2 (USP6) oncogene is a hominoid-specific gene. | Paulding, CA., et al. 2003. Proc Natl Acad Sci U S A. 100: 2507-11. PMID: 12604796
- Human and mouse proteases: a comparative genomic approach. | Puente, XS., et al. 2003. Nat Rev Genet. 4: 544-58. PMID: 12838346
- Cloning and enzymatic analysis of 22 novel human ubiquitin-specific proteases. | Quesada, V., et al. 2004. Biochem Biophys Res Commun. 314: 54-62. PMID: 14715245
- Regulation of ubiquitin-dependent processes by deubiquitinating enzymes. | Wilkinson, KD. 1997. FASEB J. 11: 1245-56. PMID: 9409543
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
USP32 Antibody (A-10) | sc-374465 | 200 µg/ml | $322.00 | |||
USP32 Antibody (A-10): m-IgG Fc BP-HRP Bundle | sc-529751 | 200 µg Ab; 10 µg BP | $361.00 | |||
USP32 Antibody (A-10): m-IgGκ BP-HRP Bundle | sc-522748 | 200 µg Ab, 40 µg BP | $361.00 | |||
USP32 Antibody (A-10): m-IgG2b BP-HRP Bundle | sc-550141 | 200 µg Ab; 10 µg BP | $361.00 | |||
USP32 Antibody (A-10) AC | sc-374465 AC | 500 µg/ml, 25% agarose | $424.00 | |||
USP32 Antibody (A-10) HRP | sc-374465 HRP | 200 µg/ml | $322.00 | |||
USP32 Antibody (A-10) FITC | sc-374465 FITC | 200 µg/ml | $336.00 | |||
USP32 Antibody (A-10) PE | sc-374465 PE | 200 µg/ml | $349.00 | |||
USP32 Antibody (A-10) Alexa Fluor® 488 | sc-374465 AF488 | 200 µg/ml | $364.00 | |||
USP32 Antibody (A-10) Alexa Fluor® 546 | sc-374465 AF546 | 200 µg/ml | $364.00 | |||
USP32 Antibody (A-10) Alexa Fluor® 594 | sc-374465 AF594 | 200 µg/ml | $364.00 | |||
USP32 Antibody (A-10) Alexa Fluor® 647 | sc-374465 AF647 | 200 µg/ml | $364.00 | |||
USP32 Antibody (A-10) Alexa Fluor® 680 | sc-374465 AF680 | 200 µg/ml | $364.00 | |||
USP32 Antibody (A-10) Alexa Fluor® 790 | sc-374465 AF790 | 200 µg/ml | $364.00 |