Anti-USP15 Antibody (B-5) recommended for detection of USP15 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

Anti-USP15 Antibody (B-5): sc-515688

Anti-USP15 Antibody (B-5) is rated 4.0 out of 5 by 3.
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Datasheets

    • Anti-USP15 Antibody (B-5) is a mouse monoclonal IgG2a κ USP15 antibody, cited in 2 publications, provided at 200 µg/ml
    • raised against amino acids 621-697 mapping within an internal region of USP15 of human origin
    • Anti-USP15 Antibody (B-5) is recommended for detection of USP15 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-USP15 Antibody (B-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of USP15 (B-5): sc-515688.
    • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for USP15 Antibody (B-5). This work is in progress.
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What blocking solution should I use with USP15 (B-5): sc-515688 monoclonal antibody for Western blot?

Asked by: Germaine
Thank you for your question. We recommend blocking with Blotto (either TBS Blotto A: sc-2333 or TBS Blotto B: sc-2335). Our Western blot protocol can be found here: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-02-28
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Rated 4 out of 5 by from Satisfactory Works well for western blotting. Blocking with BSA worked better.
Date published: 2017-10-12
Rated 4 out of 5 by from western blot it shows a clear band when loading >10ug THP-1 cell lysate ,though the exposure time is very long.
Date published: 2017-09-29
Rated 4 out of 5 by from Good I can detect endogenous USP15 in 293 cell lysates.
Date published: 2017-08-04
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