USP15 Antibody (B-5): sc-515688
- USP15 Antibody (B-5) is a mouse monoclonal IgG2a κ USP15 antibody, cited in 4 publications, provided at 200 µg/ml
- raised against amino acids 621-697 mapping within an internal region of USP15 of human origin
- USP15 Antibody (B-5) is recommended for detection of USP15 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-USP15 Antibody (B-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP is the preferred secondary detection reagent for USP15 Antibody (B-5) for WB and IHC(P) applications. This reagent is now offered in a bundle with USP15 Antibody (B-5) (see ordering information below).
USP15 Antibody (B-5) is a mouse monoclonal IgG2a kappa light chain antibody that detects USP15 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). USP15 (B-5) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. USP15, also known as ubiquitin specific peptidase 15 or UNPH4, is a crucial component of the ubiquitin-proteasome system, which regulates protein degradation and cellular homeostasis. USP15 is primarily located in the cytoplasm and nucleus, where USP15 plays a vital role in maintaining protein stability by removing ubiquitin moieties from target proteins, thus preventing their degradation by the proteasome. This deubiquitinating activity is essential for various cellular processes, including signal transduction and cell cycle regulation, as USP15 allows for the recycling of proteins that are otherwise marked for destruction. USP15 contains a DUSP domain that is involved in the regulation of the COP9 signalosome complex, which is important for modulating various signaling pathways. Additionally, alternative splicing of the USP15 gene results in three distinct isoforms, each potentially having unique regulatory roles in cellular functions.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
USP15 Antibody (B-5) References:
- The Regulations of Deubiquitinase USP15 and Its Pathophysiological Mechanisms in Diseases. | Chou, CK., et al. 2017. Int J Mol Sci. 18: PMID: 28245560
- The Multifaceted Roles of USP15 in Signal Transduction. | Das, T., et al. 2021. Int J Mol Sci. 22: PMID: 33946990
- USP15 antagonizes CRL4CRBN-mediated ubiquitylation of glutamine synthetase and neosubstrates. | Nguyen, TV. 2021. Proc Natl Acad Sci U S A. 118: PMID: 34583995
- USP15 regulates p66Shc stability associated with Drp1 activation in liver ischemia/reperfusion. | Tian, X., et al. 2022. Cell Death Dis. 13: 823. PMID: 36163170
- USP15 promotes cGAS activation through deubiquitylation and liquid condensation. | Shi, C., et al. 2022. Nucleic Acids Res. 50: 11093-11108. PMID: 36243958
- USP15 promotes pulmonary vascular remodeling in pulmonary hypertension in a YAP1/TAZ-dependent manner. | Wu, Z., et al. 2023. Exp Mol Med. 55: 183-195. PMID: 36635430
- Ablation of the deubiquitinase USP15 ameliorates nonalcoholic fatty liver disease and nonalcoholic steatohepatitis. | Baek, JH., et al. 2023. Exp Mol Med. 55: 1520-1530. PMID: 37394587
- K48-linked deubiquitination of VGLL4 by USP15 enhances the efficacy of tumor immunotherapy in triple-negative breast cancer. | Wang, X., et al. 2024. Cancer Lett. 588: 216764. PMID: 38431034
- USP15, activated by TFAP4 transcriptionally, stabilizes SHC1 via deubiquitination and deteriorates renal cell carcinoma. | Shi, Y., et al. 2024. Cancer Sci. 115: 2617-2629. PMID: 38847328
- In vivo CRISPR screens reveal SCAF1 and USP15 as drivers of pancreatic cancer. | Martinez, S., et al. 2024. Nat Commun. 15: 5266. PMID: 38902237
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
USP15 Antibody (B-5) | sc-515688 | 200 µg/ml | $316.00 | |||
USP15 Antibody (B-5): m-IgG Fc BP-HRP Bundle | sc-552129 | 200 µg Ab; 10 µg BP | $354.00 | |||
USP15 Antibody (B-5) AC | sc-515688 AC | 500 µg/ml, 25% agarose | $416.00 | |||
USP15 Antibody (B-5) HRP | sc-515688 HRP | 200 µg/ml | $316.00 | |||
USP15 Antibody (B-5) FITC | sc-515688 FITC | 200 µg/ml | $330.00 | |||
USP15 Antibody (B-5) PE | sc-515688 PE | 200 µg/ml | $343.00 | |||
USP15 Antibody (B-5) Alexa Fluor® 488 | sc-515688 AF488 | 200 µg/ml | $357.00 | |||
USP15 Antibody (B-5) Alexa Fluor® 546 | sc-515688 AF546 | 200 µg/ml | $357.00 | |||
USP15 Antibody (B-5) Alexa Fluor® 594 | sc-515688 AF594 | 200 µg/ml | $357.00 | |||
USP15 Antibody (B-5) Alexa Fluor® 647 | sc-515688 AF647 | 200 µg/ml | $357.00 | |||
USP15 Antibody (B-5) Alexa Fluor® 680 | sc-515688 AF680 | 200 µg/ml | $357.00 | |||
USP15 Antibody (B-5) Alexa Fluor® 790 | sc-515688 AF790 | 200 µg/ml | $357.00 |