USP15 Antibody (2D5): sc-100629

USP15 Antibody (2D5) is a mouse monoclonal IgG1 kappa light chain antibody that detects USP15 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). USP15 Antibody (2D5) is available in a non-conjugated form, allowing for versatile experimental use. USP15, also known as ubiquitin specific peptidase 15 or UNPH4, is a crucial member of the peptidase C19 family, playing a significant role in the ubiquitin-proteasome system, which is essential for regulating protein degradation and maintaining cellular homeostasis. USP15 is primarily located in the cytoplasm and nucleus, where USP15 performs its function as a deubiquitinating enzyme, cleaving ubiquitin residues from ubiquitinylated proteins and ubiquitin-fused precursors. By doing so, USP15 protects these proteins from proteasomal degradation, thereby influencing various cellular processes such as signal transduction, transcriptional activation, and cell cycle progression. USP15 is present in key tissues, including the kidney, liver, placenta, ovary, lung, thymus, heart, and skeletal muscle, underscoring USP15′s importance in diverse physiological functions. Additionally, USP15 contains one DUSP domain, which is integral to USP15′s interaction with the COP9 signalosome complex, further highlighting USP15′s regulatory capabilities within the cell. Notably, three isoforms of USP15 exist due to alternative splicing, adding another layer of complexity to USP15′s functional repertoire.

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USP15 Antibody (2D5) References:

1. Identification, functional characterization, and chromosomal localization of USP15, a novel human ubiquitin-specific protease related to the UNP oncoprotein, and a systematic nomenclature for human ubiquitin-specific proteases.  |  Baker, RT., et al. 1999. Genomics. 59: 264-74. PMID: 10444327
2. Isolation and characterization of the mouse ubiquitin-specific protease Usp15.  |  Angelats, C., et al. 2003. Mamm Genome. 14: 31-46. PMID: 12532266
3. The zinc finger of the CSN-associated deubiquitinating enzyme USP15 is essential to rescue the E3 ligase Rbx1.  |  Hetfeld, BK., et al. 2005. Curr Biol. 15: 1217-21. PMID: 16005295
4. Solution structure of the human ubiquitin-specific protease 15 DUSP domain.  |  de Jong, RN., et al. 2006. J Biol Chem. 281: 5026-31. PMID: 16298993
5. CSN controls NF-kappaB by deubiquitinylation of IkappaBalpha.  |  Schweitzer, K., et al. 2007. EMBO J. 26: 1532-41. PMID: 17318178
6. Ubiquitin-dependent proteolysis of the microtubule end-binding protein 1, EB1, is controlled by the COP9 signalosome: possible consequences for microtubule filament stability.  |  Peth, A., et al. 2007. J Mol Biol. 368: 550-63. PMID: 17350042
7. USP15 in Cancer and Other Diseases: From Diverse Functionsto Therapeutic Targets.  |  Li, YC., et al. 2022. Biomedicines. 10: PMID: 35203682
8. USP15 negatively regulates lung cancer progression through the TRAF6-BECN1 signaling axis for autophagy induction.  |  Kim, MJ., et al. 2022. Cell Death Dis. 13: 348. PMID: 35422093
9. Loss of the receptors ER, PR and HER2 promotes USP15-dependent stabilization of PARP1 in triple-negative breast cancer.  |  Sun, X., et al. 2023. Nat Cancer. 4: 716-733. PMID: 37012401
10. TRIM21/USP15 balances ACSL4 stability and the imatinib resistance of gastrointestinal stromal tumors.  |  Cui, Z., et al. 2024. Br J Cancer. 130: 526-541. PMID: 38182686
11. The deubiquitinase USP15 drives malignant progression of gastric cancer through glucose metabolism remodeling.  |  Huangfu, L., et al. 2024. J Exp Clin Cancer Res. 43: 235. PMID: 39164728
12. Prediction of the coding sequences of unidentified human genes. IX. The complete sequences of 100 new cDNA clones from brain which can code for large proteins in vitro.  |  Nagase, T., et al. 1998. DNA Res. 5: 31-9. PMID: 9628581

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-USP15 Antibody (2D5) has 12 citations in a variety of scientific publications.

Primary antibodies like Anti-USP15 Antibody (2D5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

USP15 Antibody (2D5) Specifications

Alternate Product Name: USP15

Product Clone Name: 2D5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-236

Epitope Target: USP15

Epitope Species: human

Product Reactivity/Detection: USP15

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 100 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 12 citations in various scientific publications

Available Conjugates/Forms for USP15 Antibody (2D5): N/A

The preferred USP15 antibody is USP15 Antibody (B-5): sc-515688.