USP11 Antibody (C-6): sc-365528

USP11 Antibody (C-6) is a mouse monoclonal IgG1 kappa light chain antibody that detects USP11 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). USP11 Antibody (C-6) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. USP11, also known as ubiquitin specific peptidase 11 or UHX1, is a crucial 920 amino acid deubiquitinating enzyme that plays a significant role in the ubiquitin (Ub) pathway, which regulates numerous intracellular processes such as signal transduction, transcriptional activation, and cell cycle progression. Located primarily in the nucleus, USP11 interacts with key proteins, including Ran binding protein M and the tumor suppressor BRCA2, to modulate their ubiquitination status. This interaction prevents ubiquitination of these proteins and facilitates the removal of ubiquitin residues that may hinder their function. USP11′s ability to deubiquitinate BRCA2 is particularly important in tumor suppression, as this process may influence cellular response to DNA damage. Additionally, USP11 has been implicated in various X-linked retinal diseases, highlighting USP11′s significance in both cancer biology and genetic disorders.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

USP11 Antibody (C-6) References:

1. Structural and functional characterization of the USP11 deubiquitinating enzyme, which interacts with the RanGTP-associated protein RanBPM.  |  Ideguchi, H., et al. 2002. Biochem J. 367: 87-95. PMID: 12084015
2. BRCA2 is ubiquitinated in vivo and interacts with USP11, a deubiquitinating enzyme that exhibits prosurvival function in the cellular response to DNA damage.  |  Schoenfeld, AR., et al. 2004. Mol Cell Biol. 24: 7444-55. PMID: 15314155
3. The deubiquitinating enzyme USP11 controls an IkappaB kinase alpha (IKKalpha)-p53 signaling pathway in response to tumor necrosis factor alpha (TNFalpha).  |  Yamaguchi, T., et al. 2007. J Biol Chem. 282: 33943-8. PMID: 17897950
4. USP11 promotes growth and metastasis of colorectal cancer via PPP1CA-mediated activation of ERK/MAPK signaling pathway.  |  Sun, H., et al. 2019. EBioMedicine. 48: 236-247. PMID: 31521612
5. USP11 regulates autophagy-dependent ferroptosis after spinal cord ischemia-reperfusion injury by deubiquitinating Beclin 1.  |  Rong, Y., et al. 2022. Cell Death Differ. 29: 1164-1175. PMID: 34839355
6. The deubiquitinase USP11 ameliorates intervertebral disc degeneration by regulating oxidative stress-induced ferroptosis via deubiquitinating and stabilizing Sirt3.  |  Zhu, J., et al. 2023. Redox Biol. 62: 102707. PMID: 37099926
7. USP11-mediated LSH deubiquitination inhibits ferroptosis in colorectal cancer through epigenetic activation of CYP24A1.  |  Duan, J., et al. 2023. Cell Death Dis. 14: 402. PMID: 37414755
8. USP11 potentiates HGF/AKT signaling and drives metastasis in hepatocellular carcinoma.  |  Chen, J., et al. 2024. Oncogene. 43: 123-135. PMID: 37973952
9. USP11 Exacerbates Radiation-Induced Pneumonitis by Activating Endothelial Cell Inflammatory Response via OTUD5-STING Signaling.  |  Tang, Y., et al. 2024. Int J Radiat Oncol Biol Phys. 119: 1261-1274. PMID: 38364946
10. USP11 promotes prostate cancer progression by up-regulating AR and c-Myc activity.  |  Pornour, M., et al. 2024. Proc Natl Acad Sci U S A. 121: e2403331121. PMID: 39052835
11. A ubiquitin C-terminal hydrolase gene on the proximal short arm of the X chromosome: implications for X-linked retinal disorders.  |  Swanson, DA., et al. 1996. Hum Mol Genet. 5: 533-8. PMID: 8845848
12. UHX1 and PCTK1: precise characterisation and localisation within a gene-rich region in Xp11.23 and evaluation as candidate genes for retinal diseases mapped to Xp21.1-p11.2.  |  Brandau, O., et al. 1998. Eur J Hum Genet. 6: 459-66. PMID: 9801870

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-USP11 Antibody (C-6) has 13 citations in a variety of scientific publications.

Primary antibodies like Anti-USP11 Antibody (C-6) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

USP11 Antibody (C-6) Specifications

Alternate Product Name: USP11

Product Clone Name: C-6

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 661-740

Epitope Target: USP11

Epitope Species: human

Product Reactivity/Detection: USP11

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 13 citations in various scientific publications

Available Conjugates/Forms for USP11 Antibody (C-6): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin