USF-2 Antibody (E-3): sc-518074

USF-2 Antibody (E-3) is a mouse monoclonal IgG2a kappa light chain antibody that detects USF-2 of mouse and rat origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). USF-2 (E-3) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. USF-2 plays a crucial role in transcriptional regulation as a member of the upstream stimulatory factor family, which also includes USF-1. USF-2 is characterized by a helix-loop-helix domain, which is essential for DNA binding, and a leucine repeat that enhances this binding capability. USF-2 is primarily located in the nucleus, where USF-2 binds to the core sequence CACGTG in the adenovirus late promoter, facilitating the transcription of various genes. USF-2′s ability to form homo- and heterodimers with USF-1 allows for a versatile regulatory mechanism, as different cell types exhibit varying ratios of these dimers, thereby influencing gene expression patterns. Understanding USF-2 dynamics and interactions is vital for elucidating USF-2′s role in cellular processes and potential implications in diseases related to transcriptional dysregulation.

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USF-2 Antibody (E-3) References:

1. The IGF2 receptor is a USF2-specific target in nontumorigenic mammary epithelial cells but not in breast cancer cells.  |  Szentirmay, MN., et al. 2003. J Biol Chem. 278: 37231-40. PMID: 12857727
2. The basic helix-loop-helix/leucine zipper transcription factor USF2 integrates serum-induced PAI-1 expression and keratinocyte growth.  |  Qi, L., et al. 2014. J Cell Biochem. 115: 1840-7. PMID: 24905330
3. Cyclin-Dependent Kinase 5 (CDK5)-Mediated Phosphorylation of Upstream Stimulatory Factor 2 (USF2) Contributes to Carcinogenesis.  |  Chi, TF., et al. 2019. Cancers (Basel). 11: PMID: 31013770
4. Loss of USF2 promotes proliferation, migration and mitophagy in a redox-dependent manner.  |  Chi, TF., et al. 2020. Redox Biol. 37: 101750. PMID: 33059314
5. USF2 reduces BMP3 expression via transcriptional activation of miR-34a, thus promoting osteogenic differentiation of BMSCs.  |  Zeng, HB., et al. 2021. J Bone Miner Metab. 39: 997-1008. PMID: 34350522
6. Upstream stimulatory factor 2 (USF2) induced upregulation of triggering receptor expressed on myeloid cells 1 (TREM1) promotes endometritis by regulating toll-like receptor (TLR) 2/4-nuclear factor-kappaB (NF-κB) signaling pathway.  |  Zhang, M., et al. 2022. Bioengineered. 13: 3609-3619. PMID: 35100093
7. USF2-mediated upregulation of TXNRD1 contributes to hepatocellular carcinoma progression by activating Akt/mTOR signaling.  |  Huang, WY., et al. 2022. Cell Death Dis. 13: 917. PMID: 36319631
8. USF2 activates RhoB/ROCK pathway by transcriptional inhibition of miR-206 to promote pyroptosis in septic cardiomyocytes.  |  Dong, W., et al. 2024. Mol Cell Biochem. 479: 1093-1108. PMID: 37347361
9. Usf2 Deficiency Promotes Autophagy to Alleviate Cerebral Ischemia-Reperfusion Injury Through Suppressing YTHDF1-m6A-Mediated Cdc25A Translation.  |  Liu, C., et al. 2024. Mol Neurobiol. 61: 2556-2568. PMID: 37914905
10. USF2 promotes autophagy and proliferation in chronic lymphocytic leukemia by inhibiting STUB1-induced NFAT5 ubiquitination.  |  Chen, B., et al. 2024. Ann Hematol. 103: 533-544. PMID: 37950051

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-USF-2 Antibody (E-3) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-USF-2 Antibody (E-3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

USF-2 Antibody (E-3) Specifications

Alternate Product Name: USF2

Product Clone Name: E-3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 320-346

Epitope Target: USF-2

Epitope Species: mouse

Product Reactivity/Detection: USF-2 p44

Species Reactivity/Detection: mouse, rat

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for USF-2 Antibody (E-3): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, X: TransCruz Reagent

The preferred USF-2 antibody is USF-2 Antibody (5E9): sc-293443.