USF-2 Antibody (5E9): sc-293443

USF-2 Antibody (5E9) is a mouse monoclonal IgG2a kappa light chain antibody that detects USF-2 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Anti-USF-2 antibody (5E9) is available in a non-conjugated form, allowing for versatile experimental use. USF-2 plays a crucial role in cellular processes as a transcription factor that regulates gene expression by binding to specific DNA sequences, particularly the core sequence CACGTG found in the adenovirus late promoter. This function is vital for proper regulation of genes involved in cell growth and differentiation, and dysregulation can lead to various diseases, including cancer. USF-2, along with USF-1, forms homo- and heterodimers that exhibit distinct binding affinities and regulatory capabilities, which can vary significantly across different cell types. The presence of helix-loop-helix domains and a leucine repeat in USF-2′s structure is essential for DNA binding efficiency, highlighting the importance of structural integrity in transcriptional regulation. Understanding USF-2 dynamics and interactions is critical for elucidating complex mechanisms of gene regulation in health and disease.

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USF-2 Antibody (5E9) References:

1. ChREBP rather than USF2 regulates glucose stimulation of endogenous L-pyruvate kinase expression in insulin-secreting cells.  |  Wang, H. and Wollheim, CB. 2002. J Biol Chem. 277: 32746-52. PMID: 12087089
2. Definition of the transcriptional activation domain of recombinant 43-kilodalton USF.  |  Kirschbaum, BJ., et al. 1992. Mol Cell Biol. 12: 5094-101. PMID: 1406684
3. Cooperative interaction of an initiator-binding transcription initiation factor and the helix-loop-helix activator USF.  |  Roy, AL., et al. 1991. Nature. 354: 245-8. PMID: 1961251
4. The leucine zipper of TFE3 dictates helix-loop-helix dimerization specificity.  |  Beckmann, H. and Kadesch, T. 1991. Genes Dev. 5: 1057-66. PMID: 2044953
5. The adenovirus major late transcription factor USF is a member of the helix-loop-helix group of regulatory proteins and binds to DNA as a dimer.  |  Gregor, PD., et al. 1990. Genes Dev. 4: 1730-40. PMID: 2249772
6. S100A8 promotes epithelial-mesenchymal transition and metastasis under TGF-β/USF2 axis in colorectal cancer.  |  Li, S., et al. 2021. Cancer Commun (Lond). 41: 154-170. PMID: 33389821
7. Multiple forms of the human gene-specific transcription factor USF. I. Complete purification and identification of USF from HeLa cell nuclei.  |  Sawadogo, M., et al. 1988. J Biol Chem. 263: 11985-93. PMID: 3403558
8. USF2 knockdown downregulates THBS1 to inhibit the TGF-β signaling pathway and reduce pyroptosis in sepsis-induced acute kidney injury.  |  Sun, J., et al. 2022. Pharmacol Res. 176: 105962. PMID: 34756923
9. USF2 and TFEB compete in regulating lysosomal and autophagy genes.  |  Kim, J., et al. 2024. Nat Commun. 15: 8334. PMID: 39333072
10. Interaction of a gene-specific transcription factor with the adenovirus major late promoter upstream of the TATA box region.  |  Sawadogo, M. and Roeder, RG. 1985. Cell. 43: 165-75. PMID: 4075392
11. An RNA polymerase II transcription factor binds to an upstream element in the adenovirus major late promoter.  |  Carthew, RW., et al. 1985. Cell. 43: 439-48. PMID: 4075400
12. Ubiquitous expression of the 43- and 44-kDa forms of transcription factor USF in mammalian cells.  |  Sirito, M., et al. 1994. Nucleic Acids Res. 22: 427-33. PMID: 8127680

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-USF-2 Antibody (5E9) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-USF-2 Antibody (5E9) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

USF-2 Antibody (5E9) Specifications

Alternate Product Name: USF-2

Product Clone Name: 5E9

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 1-100

Epitope Target: USF-2

Epitope Species: human

Product Reactivity/Detection: USF-2

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 100 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for USF-2 Antibody (5E9): N/A

The preferred USF-2 antibody is USF-2 Antibody (E-3): sc-518074.