USF-1 Antibody (B-9): sc-390033

USF-1 Antibody (B-9) is a mouse monoclonal IgG1 antibody that detects USF-1 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). USF-1, a member of the upstream stimulatory factor family, plays a crucial role in the regulation of gene expression by binding to specific DNA sequences, particularly the core sequence CACGTG found in the adenovirus late promoter. This binding is essential for the transcriptional activation of various genes, highlighting USF-1′s importance in cellular processes such as growth, differentiation, and response to environmental signals. USF-1′s ability to form homo- and heterodimers with USF-2 allows for a versatile regulatory mechanism, as different cell types exhibit varying ratios of these dimers, which can influence the transcriptional output. Additionally, USF-1 contains a helix-loop-helix domain and a leucine repeat that are critical for DNA binding efficiency, further underscoring USF-1′s significance in transcriptional regulation. Anti-USF-1 antibody (B-9) is an invaluable tool for researchers studying gene regulation and cellular signaling pathways.

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USF-1 Antibody (B-9) References:

1. DNA-binding activity of the transcription factor upstream stimulatory factor 1 (USF-1) is regulated by cyclin-dependent phosphorylation.  |  Cheung, E., et al. 1999. Biochem J. 344 Pt 1: 145-52. PMID: 10548544
2. The Usf-1 transcription factor is a novel target for the stress-responsive p38 kinase and mediates UV-induced Tyrosinase expression.  |  Galibert, MD., et al. 2001. EMBO J. 20: 5022-31. PMID: 11532965
3. Promoter I of the ovine acetyl-CoA carboxylase-alpha gene: an E-box motif at -114 in the proximal promoter binds upstream stimulatory factor (USF)-1 and USF-2 and acts as an insulin-response sequence in differentiating adipocytes.  |  Travers, MT., et al. 2001. Biochem J. 359: 273-84. PMID: 11583573
4. HOXB7 expression is regulated by the transcription factors NF-Y, YY1, Sp1 and USF-1.  |  Meccia, E., et al. 2003. Biochim Biophys Acta. 1626: 1-9. PMID: 12697323
5. UV-induced expression of key component of the tanning process, the POMC and MC1R genes, is dependent on the p-38-activated upstream stimulating factor-1 (USF-1).  |  Corre, S., et al. 2004. J Biol Chem. 279: 51226-33. PMID: 15358786
6. Pea3 transcription factor cooperates with USF-1 in regulation of the murine bax transcription without binding to an Ets-binding site.  |  Firlej, V., et al. 2005. J Biol Chem. 280: 887-98. PMID: 15466854
7. Upstream stimulatory factors (USF-1/USF-2) regulate human cGMP-dependent protein kinase I gene expression in vascular smooth muscle cells.  |  Sellak, H., et al. 2005. J Biol Chem. 280: 18425-33. PMID: 15741164
8. Regulation of IL-10 expression by upstream stimulating factor (USF-1) in glioma-associated microglia.  |  Zhang, L., et al. 2007. J Neuroimmunol. 184: 188-97. PMID: 17289164
9. In vivo and in vitro tools to identify and study transcriptional regulation of USF-1 target genes.  |  Galibert, MD. and Corre, S. 2010. Methods Mol Biol. 647: 339-55. PMID: 20694678
10. Activation of the MHC class II transactivator CIITA by interferon-gamma requires cooperative interaction between Stat1 and USF-1.  |  Muhlethaler-Mottet, A., et al. 1998. Immunity. 8: 157-66. PMID: 9491997

The preferred USF-1 antibody is USF-1 Antibody (G-2): sc-390027.