USF-1 Antibody (G-2): sc-390027

USF-1 Antibody (G-2) is a mouse monoclonal IgG2b kappa light chain antibody that detects USF-1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). USF-1 (G-2) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. USF-1 plays a crucial role in regulating gene expression, particularly in response to cellular signals, by binding to specific DNA sequences and facilitating target gene transcription. This function is vital for processes such as cell growth, differentiation, and stress response, making USF-1 an important factor in maintaining cellular homeostasis and influencing developmental pathways. USF-1 interacts with other proteins, including the c-Myc oncogene, which further underscores USF-1′s significance in transcriptional regulation and potential implications in cancer biology. USF-1′s ability to form homo- and heterodimers with USF-2 allows for a versatile regulatory mechanism, adapting to specific needs of different cell types and conditions.

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USF-1 Antibody (G-2) References:

1. Hematopoietic expression of HOXB4 is regulated in normal and leukemic stem cells through transcriptional activation of the HOXB4 promoter by upstream stimulating factor (USF)-1 and USF-2.  |  Giannola, DM., et al. 2000. J Exp Med. 192: 1479-90. PMID: 11085749
2. USF-1 and USF-2 trans-repress IL-1beta-induced iNOS transcription in mesangial cells.  |  Gupta, AK. and Kone, BC. 2002. Am J Physiol Cell Physiol. 283: C1065-72. PMID: 12225970
3. Definition of the transcriptional activation domain of recombinant 43-kilodalton USF.  |  Kirschbaum, BJ., et al. 1992. Mol Cell Biol. 12: 5094-101. PMID: 1406684
4. Plasminogen activator inhibitor-1 promoter activity in adipocytes is not influenced by the 4 G/5 G promoter polymorphism and is regulated by a USF-1/2 binding site immediately preceding the polymorphic region.  |  Zietz, B., et al. 2004. J Mol Endocrinol. 32: 155-63. PMID: 14765999
5. Regulation of IL-10 expression by upstream stimulating factor (USF-1) in glioma-associated microglia.  |  Zhang, L., et al. 2007. J Neuroimmunol. 184: 188-97. PMID: 17289164
6. Cooperative interaction of an initiator-binding transcription initiation factor and the helix-loop-helix activator USF.  |  Roy, AL., et al. 1991. Nature. 354: 245-8. PMID: 1961251
7. The leucine zipper of TFE3 dictates helix-loop-helix dimerization specificity.  |  Beckmann, H. and Kadesch, T. 1991. Genes Dev. 5: 1057-66. PMID: 2044953
8. The adenovirus major late transcription factor USF is a member of the helix-loop-helix group of regulatory proteins and binds to DNA as a dimer.  |  Gregor, PD., et al. 1990. Genes Dev. 4: 1730-40. PMID: 2249772
9. Multiple forms of the human gene-specific transcription factor USF. I. Complete purification and identification of USF from HeLa cell nuclei.  |  Sawadogo, M., et al. 1988. J Biol Chem. 263: 11985-93. PMID: 3403558
10. Interaction of a gene-specific transcription factor with the adenovirus major late promoter upstream of the TATA box region.  |  Sawadogo, M. and Roeder, RG. 1985. Cell. 43: 165-75. PMID: 4075392
11. An RNA polymerase II transcription factor binds to an upstream element in the adenovirus major late promoter.  |  Carthew, RW., et al. 1985. Cell. 43: 439-48. PMID: 4075400
12. Ubiquitous expression of the 43- and 44-kDa forms of transcription factor USF in mammalian cells.  |  Sirito, M., et al. 1994. Nucleic Acids Res. 22: 427-33. PMID: 8127680

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-USF-1 Antibody (G-2) has 11 citations in a variety of scientific publications.

Primary antibodies like Anti-USF-1 Antibody (G-2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

USF-1 Antibody (G-2) Specifications

Alternate Product Name: USF1

Product Clone Name: G-2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 75-160

Epitope Target: USF-1

Epitope Species: human

Product Reactivity/Detection: USF-1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: canine, bovine and porcine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 11 citations in various scientific publications

Available Conjugates/Forms for USF-1 Antibody (G-2): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, X: TransCruz Reagent