Anti-ULBP3 Antibody (D-1) recommended for detection of ULBP3 of human origin by WB, IP, IF and ELISA

Anti-ULBP3 Antibody (D-1): sc-390844

Anti-ULBP3 Antibody (D-1) is rated 3.0 out of 5 by 2.
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Datasheets

    • Anti-ULBP3 Antibody (D-1) is a mouse monoclonal IgG1 κ ULBP3 antibody, cited in 3 publications, provided at 200 µg/ml
    • raised against amino acids 173-217 mapping near the C-terminus of ULBP3 of human origin
    • ULBP3 Antibody (D-1) is recommended for detection of ULBP3 of human origin by WB, IP, IF and ELISA
    • Anti-ULBP3 Antibody (D-1) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of ULBP3 (D-1): sc-390844.
    • m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for ULBP3 Antibody (D-1) for WB applications. These reagents are now offered in bundles with ULBP3 Antibody (D-1) (see ordering information below).
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What is the recommended starting dilution for Western blot for sc-390844: ULBP3 (D-1) monoclonal antibody?

Asked by: jenniferc
The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, is available in the recommended protocol: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-22
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Rated 1 out of 5 by from Tested on HFFF by FACS This antibody was tested on HFFF cells by FACS and did not give a signal, even at dilution 1:25
Date published: 2018-11-19
Rated 5 out of 5 by from Produced positive Western blot data of ULBP3 Produced positive Western blot data of ULBP3 expression in HeLa and Hep G2 whole cell lysates. -SCBT QC
Date published: 2014-01-01
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