UGT1A Antibody (B-4): sc-271268

UGT1A Antibody (B-4) is a mouse monoclonal IgG1 kappa light chain antibody that detects UGT1A protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). UGT1A (B-4) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. UGT1A proteins, which are part of the UDP-glucuronosyltransferase superfamily, play a crucial role in the glucuronidation process, a key pathway for detoxifying bile acids and other xenobiotics. This process is vital for maintaining homeostasis within the body, as glucuronidation facilitates the elimination of potentially harmful substances. The UGT1A locus encodes at least ten distinct proteins (UGT1A1-UGT1A10), each exhibiting unique tissue-specific expression patterns influenced by various nuclear receptors, including pregnane X receptor (PXR) and constitutive androstane receptor (CAR). These interactions are essential for regulating the metabolism of drugs and other compounds, and deficiencies in UGT expression or activity can lead to serious genetic disorders such as Crigler-Najjar syndrome and Gilbert syndrome. Using anti-UGT1A antibody (B-4) in research provides valuable insights into drug metabolism mechanisms and UGT1A proteins′ physiological roles in human health.

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UGT1A Antibody (B-4) References:

1. Prolonged unconjugated hyperbilirubinemia associated with breast milk and mutations of the bilirubin uridine diphosphate- glucuronosyltransferase gene.  |  Maruo, Y., et al. 2000. Pediatrics. 106: E59. PMID: 11061796
2. Rapid proteasomal degradation of translocation-deficient UDP-glucuronosyltransferase 1A1 proteins in patients with Crigler-Najjar type II.  |  Ohnishi, A. and Emi, Y. 2003. Biochem Biophys Res Commun. 310: 735-41. PMID: 14550264
3. Chromosomal assignment of human phenol and bilirubin UDP-glucuronosyltransferase genes (UGT1A-subfamily).  |  Moghrabi, N., et al. 1992. Ann Hum Genet. 56: 81-91. PMID: 1503396
4. Comparative genomics analysis of human sequence variation in the UGT1A gene cluster.  |  Maitland, ML., et al. 2006. Pharmacogenomics J. 6: 52-62. PMID: 16314881
5. Monospecific antipeptide antibodies against human hepatic UDP-glucuronosyltransferase 1A subfamily (UGT1A) isoforms.  |  Ikushiro, S., et al. 2006. Drug Metab Pharmacokinet. 21: 70-4. PMID: 16547396
6. Comprehensive analysis of UGT1A polymorphisms predictive for pharmacokinetics and treatment outcome in patients with non-small-cell lung cancer treated with irinotecan and cisplatin.  |  Han, JY., et al. 2006. J Clin Oncol. 24: 2237-44. PMID: 16636344
7. Genetic variability, haplotypes, and htSNPs for exons 1 at the human UGT1A locus.  |  Thomas, SS., et al. 2006. Hum Mutat. 27: 717. PMID: 16786511
8. Variability and function of family 1 uridine-5'-diphosphate glucuronosyltransferases (UGT1A).  |  Strassburg, CP., et al. 2008. Crit Rev Clin Lab Sci. 45: 485-530. PMID: 19003600
9. Cross-Talk between Alternatively Spliced UGT1A Isoforms and Colon Cancer Cell Metabolism.  |  Audet-Delage, Y., et al. 2017. Mol Pharmacol. 91: 167-177. PMID: 28049773
10. Genetic defects at the UGT1 locus associated with Crigler-Najjar type I disease, including a prenatal diagnosis.  |  Ciotti, M., et al. 1997. Am J Med Genet. 68: 173-8. PMID: 9028453
11. The novel UGT1 gene complex links bilirubin, xenobiotics, and therapeutic drug metabolism by encoding UDP-glucuronosyltransferase isozymes with a common carboxyl terminus.  |  Owens, IS., et al. 1996. J Pharmacokinet Biopharm. 24: 491-508. PMID: 9131487
12. Differential down-regulation of the UDP-glucuronosyltransferase 1A locus is an early event in human liver and biliary cancer.  |  Strassburg, CP., et al. 1997. Cancer Res. 57: 2979-85. PMID: 9230212

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-UGT1A Antibody (B-4) has 11 citations in a variety of scientific publications.

Primary antibodies like Anti-UGT1A Antibody (B-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

UGT1A Antibody (B-4) Specifications

Alternate Product Name: UGT1A1

Product Clone Name: B-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 234-533

Epitope Target: UGT1A

Epitope Species: human

Product Reactivity/Detection: UGT1A family members

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 11 citations in various scientific publications

Available Conjugates/Forms for UGT1A Antibody (B-4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred UGT1A7 antibody is UGT1A7 Antibody (B-12): sc-377075.