UCH-L1 Antibody (C-4): sc-271639

UCH-L1 Antibody (C-4) is a mouse monoclonal IgG1 kappa light chain antibody that detects UCH-L1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). UCH-L1 (C-4) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. UCH-L1, or ubiquitin C-terminal hydrolase L1, plays a crucial role in the ubiquitin-proteasome pathway by hydrolyzing ubiquitin adducts to release free ubiquitin, which is essential for protein degradation and cellular homeostasis. UCH-L1 is predominantly expressed in neurons and neuroendocrine cells, with significant localization in the substantia nigra, an area critically involved in motor control. UCH-L1 presence in Lewy bodies, which are pathological hallmarks of Parkinson′s disease, highlights UCH-L1′s potential importance in neurodegenerative disorders. The gene encoding UCH-L1 is located on human chromosome 4p13, and human UCH-L1 consists of 233 amino acids, exhibiting a high degree of conservation with UCH-L3, another member of the UCH family, which underscores UCH-L1′s evolutionary significance. Anti-UCH-L1 antibody (C-4) is an invaluable tool for researchers investigating the role of ubiquitin-mediated processes in health and disease.

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UCH-L1 Antibody (C-4) References:

1. Intron-exon structure of ubiquitin c-terminal hydrolase-L1.  |  Leroy, E., et al. 1998. DNA Res. 5: 397-400. PMID: 10048490
2. The gene for human neurone specific ubiquitin C-terminal hydrolase (UCHL1, PGP9.5) maps to chromosome 4p14.  |  Edwards, YH., et al. 1991. Ann Hum Genet. 55: 273-8. PMID: 1840236
3. UCH-L1 inhibition involved in CREB dephosphorylation in hippocampal slices.  |  Xie, M., et al. 2014. J Mol Neurosci. 53: 59-68. PMID: 24323362
4. The neuron-specific protein PGP 9.5 is a ubiquitin carboxyl-terminal hydrolase.  |  Wilkinson, KD., et al. 1989. Science. 246: 670-3. PMID: 2530630
5. Detection, resolution, and nomenclature of multiple ubiquitin carboxyl-terminal esterases from bovine calf thymus.  |  Mayer, AN. and Wilkinson, KD. 1989. Biochemistry. 28: 166-72. PMID: 2539853
6. UCH-L1 involved in regulating the degradation of EGFR and promoting malignant properties in drug-resistant breast cancer.  |  Jin, Y., et al. 2015. Int J Clin Exp Pathol. 8: 12500-8. PMID: 26722437
7. Ubiquitin C-Terminal Hydrolase L1 (UCH-L1) Promotes Hippocampus-Dependent Memory via Its Deubiquitinating Effect on TrkB.  |  Guo, YY., et al. 2017. J Neurosci. 37: 5978-5995. PMID: 28500221
8. UCH-L1-mediated Down-regulation of Estrogen Receptor α Contributes to Insensitivity to Endocrine Therapy for Breast Cancer.  |  Chen, XS., et al. 2020. Theranostics. 10: 1833-1848. PMID: 32042339
9. Performance of glial fibrillary acidic protein (GFAP) and ubiquitin carboxy-terminal hydrolase L1 (UCH-L1) biomarkers in predicting CT scan results and neurological outcomes in children with traumatic brain injury (BRAINI-2 paediatric study): protocol of a European prospective multicentre study.  |  Lorton, F., et al. 2024. BMJ Open. 14: e083531. PMID: 38754888
10. Early GFAP and UCH-L1 point-of-care biomarker measurements for the prediction of traumatic brain injury and progression in patients with polytrauma and hemorrhagic shock.  |  Sperry, JL., et al. 2024. J Neurosurg. 1-10. PMID: 39076152
11. Diagnostic Performance of GFAP, UCH-L1, and MAP-2 Within 30 and 60 Minutes of Traumatic Brain Injury.  |  Papa, L., et al. 2024. JAMA Netw Open. 7: e2431115. PMID: 39230905
12. Isolation of PGP 9.5, a new human neurone-specific protein detected by high-resolution two-dimensional electrophoresis.  |  Doran, JF., et al. 1983. J Neurochem. 40: 1542-7. PMID: 6343558

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-UCH-L1 Antibody (C-4) has 24 citations in a variety of scientific publications.

Primary antibodies like Anti-UCH-L1 Antibody (C-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

UCH-L1 Antibody (C-4) Specifications

Alternate Product Name: UCHL1

Product Clone Name: C-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 184-223

Epitope Target: UCH-L1

Epitope Species: human

Product Reactivity/Detection: UCH-L1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 24 citations in various scientific publications

Available Conjugates/Forms for UCH-L1 Antibody (C-4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin