Ubiquitin Antibody (A-5): sc-166553

Ubiquitin Antibody (A-5) is a mouse monoclonal IgG2b kappa light chain antibody engineered to recognize full-length human Ubiquitin protein, encompassing amino acids 1-76. Ubiquitin monoclonal antibody (A-5) detects Ubiquitin from mouse, rat, and human origins across various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Available in both non-conjugated form and multiple conjugated variants such as agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and various Alexa Fluor® conjugates, anti-Ubiquitin antibody (A-5) offers versatile solutions tailored to diverse experimental needs. Ubiquitin is a highly conserved small protein composed of 76 amino acids, characterized by a compact, beta-grasp fold that is crucial for interacting with cellular proteins and enzymes. This structural integrity allows Ubiquitin to form covalent isopeptide bonds with lysine residues on target proteins, facilitating polyubiquitin chain formation essential for signaling pathways related to protein degradation, DNA repair, and cell cycle regulation. The ability of Ubiquitin to adopt different conformations enables tagging abnormal, foreign, and improperly folded proteins for degradation by the 26S Proteasome, maintaining cellular homeostasis and protein quality control. Additionally, Ubiquitin′s structural features enable participation in signal transduction cascade regulation by modulating stability and activity of key regulatory proteins such as IκB-α and p27. Ubiquitin′s precise structural configuration ensures specific and efficient interactions, making anti-Ubiquitin antibody (A-5) valuable for investigating protein turnover, cellular signaling mechanisms, and regulatory networks that sustain cellular function and integrity.

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Ubiquitin Antibody (A-5) References:

1. Ubiquitination and endocytosis of plasma membrane proteins: role of Nedd4/Rsp5p family of ubiquitin-protein ligases.  |  Rotin, D., et al. 2000. J Membr Biol. 176: 1-17. PMID: 10882424
2. Domains of the Rsp5 ubiquitin-protein ligase required for receptor-mediated and fluid-phase endocytosis.  |  Dunn, R. and Hicke, L. 2001. Mol Biol Cell. 12: 421-35. PMID: 11179425
3. U box proteins as a new family of ubiquitin-protein ligases.  |  Hatakeyama, S., et al. 2001. J Biol Chem. 276: 33111-20. PMID: 11435423
4. Cloning and characterization of an androgen receptor N-terminal-interacting protein with ubiquitin-protein ligase activity.  |  Beitel, LK., et al. 2002. J Mol Endocrinol. 29: 41-60. PMID: 12200228
5. Adenovirus protein involved in virus internalization recruits ubiquitin-protein ligases.  |  Galinier, R., et al. 2002. Biochemistry. 41: 14299-305. PMID: 12450395
6. Biochemical analysis of Parkinson's disease-causing variants of Parkin, an E3 ubiquitin-protein ligase with monoubiquitylation capacity.  |  Hampe, C., et al. 2006. Hum Mol Genet. 15: 2059-75. PMID: 16714300
7. The role of ubiquitin-protein ligases in neurodegenerative disease.  |  Ardley, HC. and Robinson, PA. 2004. Neurodegener Dis. 1: 71-87. PMID: 16908979
8. A ubiquitin-protein ligase specific for type III protein substrates.  |  Heller, H. and Hershko, A. 1990. J Biol Chem. 265: 6532-5. PMID: 2324089
9. Role of the C-terminus of Saccharomyces cerevisiae ubiquitin-conjugating enzyme (Rad6) in substrate and ubiquitin-protein-ligase (E3-R) interactions.  |  Raboy, B. and Kulka, RG. 1994. Eur J Biochem. 221: 247-51. PMID: 8168512
10. The ubiquitin-protein ligase E6-associated protein (E6-AP) serves as its own substrate.  |  Nuber, U., et al. 1998. Eur J Biochem. 254: 643-9. PMID: 9688277

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Ubiquitin Antibody (A-5) has 99 citations in a variety of scientific publications.

Primary antibodies like Anti-Ubiquitin Antibody (A-5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Ubiquitin Antibody (A-5) Specifications

Alternate Product Name: Ubiquitin

Product Clone Name: A-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 1-76

Epitope Target: Ubiquitin

Epitope Species: human

Product Reactivity/Detection: Ubiquitin and polyubiquitin

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 99 citations in various scientific publications

Available Conjugates/Forms for Ubiquitin Antibody (A-5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred Ubiquitin antibody is Ubiquitin Antibody (P4D1): sc-8017.