Ubiquitin Antibody (P4D1): sc-8017
- Ubiquitin Antibody (P4D1) is a mouse monoclonal IgG1 κ Ubiquitin antibody, cited in 3,433 publications, provided at 200 µg/ml
- raised against amino acids 1-76 representing full length Ubiquitin of bovine origin
- Ubiquitin Antibody (P4D1) is recommended for detection of Ubiquitin, poly-ubiquitinated and ubiquitinated proteins of mouse, rat, human and Drosophila origin by WB, IP, IF, IHC(P), FCM and ELISA
- Ubiquitin Antibody (P4D1) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- available conjugated to biotin for WB, IHC(P) and ELISA; and to Alexa Fluor® 405 for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 405 for IF, IHC(P) and FCM
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for Ubiquitin Antibody (P4D1) for WB and IHC(P) applications. These reagents are now offered in bundles with Ubiquitin Antibody (P4D1) (see ordering information below).
Ubiquitin Antibody (P4D1) is a mouse monoclonal IgG1 kappa light chain antibody designed to detect the full-length Ubiquitin protein, encompassing amino acids 1-76 of bovine origin. Ubiquitin mouse monoclonal antibody (P4D1) recognizes Ubiquitin from mouse, rat, human, and Drosophila melanogaster sources across applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), flow cytometry (FCM), and enzyme-linked immunosorbent assay (ELISA). Anti-Ubiquitin antibody (P4D1) is available in both non-conjugated and various conjugated forms such as agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The Ubiquitin protein itself is one of the most evolutionarily conserved proteins, playing a crucial role in cellular homeostasis by targeting abnormal, foreign, and improperly folded proteins for degradation via the 26S Proteasome. Ubiquitin achieves its function through covalent attachment to substrates via an isopeptide bond between its carboxy-terminal glycine and lysine residues on target proteins, facilitating the formation of ubiquitin chains, particularly through Lysine 48 linkages, which signal for proteolysis. Beyond protein degradation, Ubiquitin also regulates signal transduction pathways through diverse polyubiquitin linkages, including Lysine 63 linkages, which are critical for non-proteolytic roles such as DNA repair and intracellular signaling. Ubiquitin modulates the stability of regulatory proteins such as IκB-α and p27, thereby influencing processes like inflammation, cell cycle progression, and DNA damage responses. Ubiquitin monoclonal antibody (P4D1) is recommended for researchers studying protein turnover, cellular signaling, and various aspects of cellular regulation.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Ubiquitin Antibody (P4D1) References:
- Ubiquitin, proteasomes, and the regulation of intracellular protein degradation. | Hochstrasser, M. 1995. Curr Opin Cell Biol. 7: 215-23. PMID: 7612274
- Role of the ubiquitin-proteasome pathway in regulating abundance of the cyclin-dependent kinase inhibitor p27. | Pagano, M., et al. 1995. Science. 269: 682-5. PMID: 7624798
- Ubiquitin and the enigma of intracellular protein degradation. | Jennissen, HP. 1995. Eur J Biochem. 231: 1-30. PMID: 7628459
- Ubiquitin in homeostasis, development and disease. | Muller, S. and Schwartz, LM. 1995. Bioessays. 17: 677-84. PMID: 7661849
- The ubiquitin-proteasome proteolytic pathway. | Ciechanover, A. 1994. Cell. 79: 13-21. PMID: 7923371
- The ubiquitin-mediated proteolytic pathway: mechanisms of recognition of the proteolytic substrate and involvement in the degradation of native cellular proteins. | Ciechanover, A. and Schwartz, AL. 1994. FASEB J. 8: 182-91. PMID: 8119489
- Protein degradation or regulation: Ub the judge. | Hochstrasser, M. 1996. Cell. 84: 813-5. PMID: 8601303
- Site-specific phosphorylation of IkappaBalpha by a novel ubiquitination-dependent protein kinase activity. | Chen, ZJ., et al. 1996. Cell. 84: 853-62. PMID: 8601309
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Ubiquitin Antibody (P4D1) | sc-8017 | 200 µg/ml | $322.00 | |||
Ubiquitin Antibody (P4D1): m-IgG Fc BP-HRP Bundle | sc-526508 | 200 µg Ab; 10 µg BP | $361.00 | |||
Ubiquitin Antibody (P4D1): m-IgG1 BP-HRP Bundle | sc-531881 | 200 µg Ab; 20 µg BP | $361.00 | |||
Ubiquitin Antibody (P4D1) AC | sc-8017 AC | 500 µg/ml, 25% agarose | $424.00 | |||
Ubiquitin Antibody (P4D1) HRP | sc-8017 HRP | 200 µg/ml | $322.00 | |||
Ubiquitin Antibody (P4D1) FITC | sc-8017 FITC | 200 µg/ml | $336.00 | |||
Ubiquitin Antibody (P4D1) PE | sc-8017 PE | 200 µg/ml | $349.00 | |||
Ubiquitin Antibody (P4D1) Alexa Fluor® 488 | sc-8017 AF488 | 200 µg/ml | $364.00 | |||
Ubiquitin Antibody (P4D1) Alexa Fluor® 546 | sc-8017 AF546 | 200 µg/ml | $364.00 | |||
Ubiquitin Antibody (P4D1) Alexa Fluor® 594 | sc-8017 AF594 | 200 µg/ml | $364.00 | |||
Ubiquitin Antibody (P4D1) Alexa Fluor® 647 | sc-8017 AF647 | 200 µg/ml | $364.00 | |||
Ubiquitin Antibody (P4D1) Alexa Fluor® 680 | sc-8017 AF680 | 200 µg/ml | $364.00 | |||
Ubiquitin Antibody (P4D1) Alexa Fluor® 790 | sc-8017 AF790 | 200 µg/ml | $364.00 | |||
Ubiquitin Antibody (P4D1) B | sc-8017 B | 200 µg/ml | $326.00 | |||
Ubiquitin Antibody (P4D1) Alexa Fluor® 405 | sc-8017 AF405 | 200 µg/ml | $364.00 |