UBE4A Antibody (B-6): sc-365904
- UBE4A Antibody (B-6) is a mouse monoclonal IgG2a κ UBE4A antibody, cited in 2 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 595-627 within an internal region of UBE4A of human origin
- UBE4A Antibody (B-6) is recommended for detection of UBE4A of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including and canine and porcine
- Anti-UBE4A Antibody (B-6) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for UBE4A Antibody (B-6) for WB and IHC(P) applications. These reagents are now offered in bundles with UBE4A Antibody (B-6) (see ordering information below).
UBE4A Antibody (B-6) is a mouse monoclonal IgG2a kappa light chain antibody that detects UBE4A of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). UBE4A monoclonal antibody (B-6) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. UBE4A, also known as ubiquitin conjugation factor E4 A, E4, UFD2, or UBOX2, is a 1,066 amino acid protein that plays a crucial role in the multiubiquitin pathway of protein degradation, which is essential for maintaining cellular homeostasis by regulating the turnover of short-lived or damaged proteins. UBE4A is primarily located in cortical neurons and tubular kidney cells, where UBE4A interacts with ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s), and ubiquitin-protein ligases (E3s) to facilitate the assembly of ubiquitin chains. Proper functioning of UBE4A is vital for processes such as cell growth and differentiation, and UBE4A dysregulation can lead to pathological conditions, including scleroderma, characterized by excessive collagen deposition. UBE4A exists in two isoforms due to alternative splicing, which may contribute to functional diversity in various cellular contexts.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
UBE4A Antibody (B-6) References:
- Expression analysis of the gene encoding for the U-box-type ubiquitin ligase UBE4A in human tissues. | Contino, G., et al. 2004. Gene. 328: 69-74. PMID: 15019985
- Ufd2, a novel autoantigen in scleroderma, regulates sister chromatid separation. | Spinette, S., et al. 2004. Cell Cycle. 3: 1638-44. PMID: 15611659
- The two human homologues of yeast UFD2 ubiquitination factor, UBE4A and UBE4B, are located in common neuroblastoma deletion regions and are subject to mutations in tumours. | Carén, H., et al. 2006. Eur J Cancer. 42: 381-7. PMID: 16386891
- Differentially expressed genes between high-risk human papillomavirus types in human cervical cancer cells. | Vazquez-Ortiz, G., et al. 2007. Int J Gynecol Cancer. 17: 484-91. PMID: 17309674
- [Differential expression of USP2, USP14 and UBE4A between ovarian serous cystadenocarcinoma and adjacent normal tissues]. | Yang, Y., et al. 2007. Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 23: 504-6. PMID: 17553343
- Autoantibodies against ubiquitination factor E4A (UBE4A) are associated with severity of Crohn's disease. | Sakiyama, T., et al. 2008. Inflamm Bowel Dis. 14: 310-7. PMID: 18069675
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
UBE4A Antibody (B-6) | sc-365904 | 200 µg/ml | $316.00 | |||
UBE4A Antibody (B-6): m-IgG Fc BP-HRP Bundle | sc-529505 | 200 µg Ab; 10 µg BP | $354.00 | |||
UBE4A Antibody (B-6): m-IgGκ BP-HRP Bundle | sc-522415 | 200 µg Ab, 40 µg BP | $354.00 | |||
UBE4A Antibody (B-6) AC | sc-365904 AC | 500 µg/ml, 25% agarose | $416.00 | |||
UBE4A Antibody (B-6) HRP | sc-365904 HRP | 200 µg/ml | $316.00 | |||
UBE4A Antibody (B-6) FITC | sc-365904 FITC | 200 µg/ml | $330.00 | |||
UBE4A Antibody (B-6) PE | sc-365904 PE | 200 µg/ml | $343.00 | |||
UBE4A Antibody (B-6) Alexa Fluor® 488 | sc-365904 AF488 | 200 µg/ml | $357.00 | |||
UBE4A Antibody (B-6) Alexa Fluor® 546 | sc-365904 AF546 | 200 µg/ml | $357.00 | |||
UBE4A Antibody (B-6) Alexa Fluor® 594 | sc-365904 AF594 | 200 µg/ml | $357.00 | |||
UBE4A Antibody (B-6) Alexa Fluor® 647 | sc-365904 AF647 | 200 µg/ml | $357.00 | |||
UBE4A Antibody (B-6) Alexa Fluor® 680 | sc-365904 AF680 | 200 µg/ml | $357.00 | |||
UBE4A Antibody (B-6) Alexa Fluor® 790 | sc-365904 AF790 | 200 µg/ml | $357.00 | |||
UBE4A (B-6) Neutralizing Peptide | sc-365904 P | 100 µg/0.5 ml | $68.00 |