UBE1L Antibody (E-10): sc-376765
- UBE1L Antibody (E-10) is a mouse monoclonal IgG1 κ UBE1L antibody, cited in 1 publications, provided at 200 µg/ml
- raised against amino acids 154-357 mapping within an internal region of UBE1L of mouse origin
- recommended for detection of UBE1L of mouse, rat and human origin by WB, IP, IF and ELISA
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for UBE1L Antibody (E-10) for WB applications. These reagents are now offered in bundles with UBE1L Antibody (E-10) (see ordering information below).
UBE1L Antibody (E-10) is a mouse monoclonal IgG1 kappa light chain antibody that detects UBE1L protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). UBE1L (E-10) antibody is available as a non-conjugated anti-UBE1L antibody. UBE1L protein, also known as UBA7, plays a crucial role in the ubiquitin-proteasome system, which is essential for regulating protein degradation and maintaining cellular homeostasis. UBE1L is involved in the activation of ubiquitin through an ATP-dependent adenylation process, where UBE1L catalyzes the formation of a thioester bond between ubiquitin and a cysteine residue in the enzyme, facilitating the subsequent transfer of ubiquitin to target proteins. This process is vital for marking proteins for degradation, thereby influencing various cellular processes such as cell cycle regulation, signal transduction, and responses to stress. Notably, UBE1L is expressed in tumor cells and has been identified as a target for retinoid therapy, as UBE1L conjugates with ISG15, leading to the degradation of specific proteins and triggering apoptosis in acute promyelocytic leukemia. The ability of UBE1L to modulate protein levels through ubiquitination underscores its importance in cancer biology and therapeutic interventions.
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UBE1L Antibody (E-10) References:
- UBE1L is a retinoid target that triggers PML/RARalpha degradation and apoptosis in acute promyelocytic leukemia. | Kitareewan, S., et al. 2002. Proc Natl Acad Sci U S A. 99: 3806-11. PMID: 11891284
- Involvement of UBE1L in ISG15 conjugation during retinoid-induced differentiation of acute promyelocytic leukemia. | Pitha-Rowe, I., et al. 2004. J Biol Chem. 279: 18178-87. PMID: 14976209
- Microarray analyses uncover UBE1L as a candidate target gene for lung cancer chemoprevention. | Pitha-Rowe, I., et al. 2004. Cancer Res. 64: 8109-15. PMID: 15520223
- Human ISG15 conjugation targets both IFN-induced and constitutively expressed proteins functioning in diverse cellular pathways. | Zhao, C., et al. 2005. Proc Natl Acad Sci U S A. 102: 10200-5. PMID: 16009940
- Properties of the ISG15 E1 enzyme UbE1L. | Krug, RM., et al. 2005. Methods Enzymol. 398: 32-40. PMID: 16275317
- The interferon-inducible ubiquitin-protein isopeptide ligase (E3) EFP also functions as an ISG15 E3 ligase. | Zou, W. and Zhang, DE. 2006. J Biol Chem. 281: 3989-94. PMID: 16352599
- Ube1L and protein ISGylation are not essential for alpha/beta interferon signaling. | Kim, KI., et al. 2006. Mol Cell Biol. 26: 472-9. PMID: 16382139
- Herc5, an interferon-induced HECT E3 enzyme, is required for conjugation of ISG15 in human cells. | Dastur, A., et al. 2006. J Biol Chem. 281: 4334-8. PMID: 16407192
- Link between the ubiquitin conjugation system and the ISG15 conjugation system: ISG15 conjugation to the UbcH6 ubiquitin E2 enzyme. | Takeuchi, T., et al. 2005. J Biochem. 138: 711-9. PMID: 16428300
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
UBE1L Antibody (E-10) | sc-376765 | 200 µg/ml | $316.00 | |||
UBE1L Antibody (E-10): m-IgG Fc BP-HRP Bundle | sc-540640 | 200 µg Ab; 10 µg BP | $354.00 | |||
UBE1L Antibody (E-10): m-IgG1 BP-HRP Bundle | sc-542262 | 200 µg Ab; 20 µg BP | $354.00 |