Tyrosinase Antibody (B-3): sc-514492

Tyrosinase Antibody (B-3) is a mouse monoclonal IgG2a antibody that detects tyrosinase in human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Tyrosinase, a type I membrane protein and copper-containing enzyme, plays a crucial role in melanin production, which is essential for pigmentation in vertebrates. Anti-tyrosinase antibody (B-3) catalyzes the critical and rate-limiting step of tyrosine hydroxylation, a process vital for the biosynthesis of melanin. The proper functioning of tyrosinase is paramount, as defects in its activity can lead to various forms of albinism, highlighting its importance in skin and hair pigmentation. Additionally, tyrosinase interacts with related proteins such as TRP1 and TRP2, which are also expressed in melanocytes and contribute to melanin synthesis within melanosomes. These proteins share a similar transmembrane structure and contain metal-binding regions, but they exhibit distinct catalytic activities that are essential for the formation of melanin biopolymers. The interaction among these proteins suggests the existence of a multi-enzyme complex, where TRP1 stabilizes tyrosinase, further emphasizing the intricate regulation of melanin biosynthesis. Tyrosinase monoclonal antibody (B-3) is an invaluable tool for researchers studying pigmentation disorders and the molecular mechanisms underlying melanin production.

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Contains Mercury: All HRP conjugates (including bundle forms) contain thiomersal, a mercury compound. Do not dispose in trash. Dispose as hazardous chemical waste according to local, state and federal regulations.

Download thiomersal SDS

Tyrosinase Antibody (B-3) References:

1. Novel peptides with tyrosinase inhibitory activity.  |  Schurink, M., et al. 2007. Peptides. 28: 485-95. PMID: 17241698
2. The oxidation of catechol by tyrosinase.  |  WRIGHT, CI. and MASON, HS. 1946. J Biol Chem. 165: 45-53. PMID: 21001183
3. New halogenated phenylcoumarins as tyrosinase inhibitors.  |  Matos, MJ., et al. 2011. Bioorg Med Chem Lett. 21: 3342-5. PMID: 21514152
4. New tyrosinase inhibitors from Paecilomyces gunnii.  |  Lu, R., et al. 2014. J Agric Food Chem. 62: 11917-23. PMID: 25384266
5. Kinetic characterization of oxyresveratrol as a tyrosinase substrate.  |  Ortiz-Ruiz, CV., et al. 2015. IUBMB Life. 67: 828-36. PMID: 26450473
6. Synergistic Promotion on Tyrosinase Inhibition by Antioxidants.  |  Wang, Y., et al. 2018. Molecules. 23: PMID: 29300356
7. Inhibitory Effects of Coumarin Derivatives on Tyrosinase.  |  Roh, EJ. 2021. Molecules. 26: PMID: 33920683
8. Genetically encoded dihydroxyphenylalanine coupled with tyrosinase for strain promoted labeling.  |  George, A., et al. 2021. Bioorg Med Chem. 50: 116460. PMID: 34757293
9. The Crystal Structure of Tyrosinase from Verrucomicrobium spinosum Reveals It to Be an Atypical Bacterial Tyrosinase.  |  Fekry, M., et al. 2023. Biomolecules. 13: PMID: 37759761
10. Sources and Applications of Tyrosinase in Life Sciences.  |  Patil, M., et al. 2024. Curr Pharm Biotechnol.. PMID: 39021165

The preferred Tyrosinase antibody is Tyrosinase Antibody (T311): sc-20035.