Tyrosinase Antibody (T311): sc-20035

Tyrosinase Antibody (T311) is a mouse monoclonal IgG2a kappa light chain antibody that detects Tyrosinase protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry with paraffin-embedded sections (IHCP). Tyrosinase (T311) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Tyrosinase (TYR) is a type I membrane protein and copper-containing enzyme that plays a crucial role in melanin production, the primary pigment in vertebrates. Melanin biogenesis protects skin and eyes from UV radiation, and Tyrosinase catalyzes the critical and rate-limiting step of tyrosine hydroxylation in melanin biosynthesis. Deficiencies in Tyrosinase activity can lead to various forms of albinism, highlighting its importance in pigmentation. Tyrosinase interacts with related proteins TRP1 and TRP2, which are also expressed in melanocytes and contribute to melanin synthesis within melanosomes. These proteins share a similar transmembrane structure, featuring two metal-binding regions and a cysteine-rich epidermal growth factor motif, and are localized in the melanosomal membrane. While forming a multi-enzyme complex that aggregates together, each protein has distinct catalytic activities that contribute to the overall biosynthesis of melanin biopolymers, with TRP1 playing a role in stabilizing Tyrosinase in melanocytes.

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Tyrosinase Antibody (T311) References:

1. Mutational mapping of the catalytic activities of human tyrosinase.  |  Tripathi, RK., et al. 1992. J Biol Chem. 267: 23707-12. PMID: 1429711
2. A second tyrosinase-related protein, TRP-2, is a melanogenic enzyme termed DOPAchrome tautomerase.  |  Tsukamoto, K., et al. 1992. EMBO J. 11: 519-26. PMID: 1537333
3. Tyrosinase activated melanoma prodrugs.  |  Jawaid, S., et al. 2009. Anticancer Agents Med Chem. 9: 717-27. PMID: 19538169
4. Structure and Function of Human Tyrosinase and Tyrosinase-Related Proteins.  |  Lai, X., et al. 2018. Chemistry. 24: 47-55. PMID: 29052256
5. Cloning and expression of cDNA encoding mouse tyrosinase.  |  Shibahara, S., et al. 1986. Nucleic Acids Res. 14: 2413-27. PMID: 3008090
6. Mammalian tyrosinase--the critical regulatory control point in melanocyte pigmentation.  |  Hearing, VJ. and Jiménez, M. 1987. Int J Biochem. 19: 1141-7. PMID: 3125075
7. Tyrosinase inhibitors as potential antibacterial agents.  |  Yuan, Y., et al. 2020. Eur J Med Chem. 187: 111892. PMID: 31810785
8. Natural products as tyrosinase inhibitors.  |  Doğan, A. and Akocak, S. 2024. Enzymes. 56: 85-109. PMID: 39304292
9. Mammalian tyrosinase catalyzes three reactions in the biosynthesis of melanin.  |  Körner, A. and Pawelek, J. 1982. Science. 217: 1163-5. PMID: 6810464
10. High-molecular-weight forms of tyrosinase and the tyrosinase-related proteins: evidence for a melanogenic complex.  |  Orlow, SJ., et al. 1994. J Invest Dermatol. 103: 196-201. PMID: 8040609
11. Molecular characterization of a human tyrosinase-related-protein-2 cDNA. Patterns of expression in melanocytic cells.  |  Bouchard, B., et al. 1994. Eur J Biochem. 219: 127-34. PMID: 8306979
12. Molecular interactions within the melanogenic complex: formation of heterodimers of tyrosinase and TRP1 from B16 mouse melanoma.  |  Jiménez-Cervantes, C., et al. 1998. Biochem Biophys Res Commun. 253: 761-7. PMID: 9918801

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Tyrosinase Antibody (T311) has 102 citations in a variety of scientific publications.

Primary antibodies like Anti-Tyrosinase Antibody (T311) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Tyrosinase Antibody (T311) Specifications

Alternate Product Name: Tyrosinase

Product Clone Name: T311

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids:

Epitope Target: Tyrosinase

Epitope Species: human

Product Reactivity/Detection: Tyrosinase

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 102 citations in various scientific publications

Available Conjugates/Forms for Tyrosinase Antibody (T311): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin