tuberin Antibody (B-5): sc-271314

Tuberin Antibody (B-5) is a mouse monoclonal IgG1 kappa light chain antibody that detects tuberin protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-tuberin antibody (B-5) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Tuberin, also known as Tuberous sclerosis complex (TSC) subunit 2, plays a crucial role in cellular signaling pathways, particularly in regulating cell growth and proliferation. Tuberin (B-5) monoclonal antibody serves as a key component of the TSC complex, which acts as a tumor suppressor by inhibiting the mammalian target of rapamycin (mTOR) pathway. Dysregulation of this pathway due to mutations in the TSC2 gene, which encodes tuberin, can lead to the development of hamartomas and other tumors associated with Tuberous sclerosis complex, a genetic disorder characterized by various benign tumors and neurological issues. The interaction between tuberin and hamartin is essential for maintaining cellular homeostasis, and disruptions in this interaction can result in significant pathological consequences. Anti-tuberin antibody (B-5) serves as an invaluable tool for researchers studying TSC and related signaling pathways, providing insights into the molecular mechanisms underlying tumorigenesis and potential therapeutic targets.

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tuberin Antibody (B-5) References:

1. Antisense suppression of TSC1 gene product, hamartin, enhances neurite outgrowth in NGF-treated PC12h cells.  |  Floricel, F., et al. 2007. Brain Dev. 29: 502-9. PMID: 17376623
2. Tuberin: a stimulus-regulated tumor suppressor protein controlled by a diverse array of receptor tyrosine kinases and G protein-coupled receptors.  |  Wu, EH., et al. Neurosignals. 15: 217-27. PMID: 17389815
3. Inhibition of GTPase activating protein stimulation of Ras-p21 GTPase by the Krev-1 gene product.  |  Frech, M., et al. 1990. Science. 249: 169-71. PMID: 2164710
4. Tuberin in renal cell hypertrophy.  |  Habib, SL. 2014. Cell Cycle. 13: 869-70. PMID: 24552810
5. Human cDNAs rap1 and rap2 homologous to the Drosophila gene Dras3 encode proteins closely related to ras in the 'effector' region.  |  Pizon, V., et al. 1988. Oncogene. 3: 201-4. PMID: 3045729
6. Nucleotide sequence of a human cDNA encoding a ras-related protein (rap1B).  |  Pizon, V., et al. 1988. Nucleic Acids Res. 16: 7719. PMID: 3137530
7. Tuberin levels during cellular differentiation in brain development.  |  Fidalgo da Silva, E., et al. 2023. Differentiation. 130: 43-50. PMID: 36608575
8. Identification of tuberin, the tuberous sclerosis-2 product. Tuberin possesses specific Rap1GAP activity.  |  Wienecke, R., et al. 1995. J Biol Chem. 270: 16409-14. PMID: 7608212
9. The mouse homologue of the tuberin gene (TSC2) maps to a conserved synteny group between mouse chromosome 17 and human 16p13.3.  |  Olsson, PG., et al. 1995. Genomics. 25: 339-40. PMID: 7774953
10. The molecular genetics of tuberous sclerosis.  |  Sampson, JR. and Harris, PC. 1994. Hum Mol Genet. 3 Spec No: 1477-80. PMID: 7849741
11. Proteins regulating Ras and its relatives.  |  Boguski, MS. and McCormick, F. 1993. Nature. 366: 643-54. PMID: 8259209
12. Identification and characterization of the tuberous sclerosis gene on chromosome 16.  |  ,. 1993. Cell. 75: 1305-15. PMID: 8269512

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-tuberin Antibody (B-5) has 12 citations in a variety of scientific publications.

Primary antibodies like Anti-tuberin Antibody (B-5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

tuberin Antibody (B-5) Specifications

Alternate Product Name: TSC2

Product Clone Name: B-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-300

Epitope Target: tuberin

Epitope Species: human

Product Reactivity/Detection: tuberin

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 12 citations in various scientific publications

Available Conjugates/Forms for tuberin Antibody (B-5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred p-tuberin antibody is p-tuberin Antibody (325.Ser 1798): sc-293149.