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TTP 抗体 (H-12): sc-398904

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说明书
  • TTP抗体(H-12)是小鼠单克隆IgG2b (kappa轻链),规格为200 µg/ml
  • 特异性抗原位于human物种的TTP的C-terminus附近的氨基酸287-302之间
  • 推荐用于 mouse, rat 和 human 来源的TTP WB, IP, IF 和 ELISA检测
  • 看 TTP (A-8): sc-374305,可查看有标记的 TTP 抗体,包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790
  • 2b BP-HRP">m-IgG2b BP-HRP和m-IgGκ BP-HRP是TTP Antibody (H-12) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与TTP Antibody (H-12) 打包提供(请参阅下面的订购信息)。
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    関連項目

    Tristetraprolin(TTP),也称为Nup475和TIS11,是一种由即时早期反应基因Zfp-36编码的锌结合蛋白。通过包括血小板源性生长因子和成纤维细胞生长因子在内的有丝分裂原刺激静止的成纤维细胞,会导致TTP的丝氨酸磷酸化,并使该蛋白从细胞核迅速重新分布到细胞质。体外研究表明,TTP被p42 MAP激酶磷酸化,这表明TTP的活性可能在体内受到MAP激酶通路的调节。TTP缺失的小鼠会出现自身免疫性、炎症性关节炎和皮炎。这些状况可以通过阻断炎症介质肿瘤坏死因子-α(TNF-α)的活性来逆转,这表明TTP可能起负向调节TNF-α表达的作用。

    仅限研究使用。不适用于诊断和治疗用途。

    Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

    LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

    TTP 抗体 (H-12) 参考文献:

    1. Tristetraprolin(TTP)协调调节细胞对促炎性刺激的主要和次要反应。  |  Qiu, LQ., et al. 2015. J Leukoc Biol. 97: 723-36. PMID: 25657290
    2. RNA 结合蛋白 Tristetraprolin (TTP) 是 NLRP3 炎症小体的关键负调控因子。  |  Haneklaus, M., et al. 2017. J Biol Chem. 292: 6869-6881. PMID: 28302726
    3. 癌症中Tristetraprolin和细胞因子之间的相互影响。  |  Guo, J., et al. 2017. Anticancer Agents Med Chem. 17: 1477-1486. PMID: 28356023
    4. Cu(I)破坏了非经典锌指蛋白Tristetraprolin(TTP)的结构和功能。  |  Shimberg, GD., et al. 2017. Inorg Chem. 56: 6838-6848. PMID: 28557421
    5. Tristetraprolin在肿瘤发生中的作用。  |  Park, JM., et al. 2018. Int J Mol Sci. 19: PMID: 30380668
    6. Tristetraprolin-RNA相互作用图揭示了先天性免疫基因表达的新型TTP-RelB调控网络。  |  Tu, Y., et al. 2020. Mol Immunol. 121: 59-71. PMID: 32163759
    7. Tristetraprolin在炎症消退中的作用。  |  Rappl, P., et al. 2021. Biology (Basel). 10: PMID: 33477783
    8. TTP管理方面的进展。  |  Subhan, M. and Scully, M. 2022. Blood Rev. 55: 100945. PMID: 35216839
    9. 三聚体蛋白(TTP)调节在炎症性疾病治疗中的临床意义。  |  Snyder, BL. and Blackshear, PJ. 2022. Pharmacol Ther. 239: 108198. PMID: 35525391
    10. 对晚发性阿尔茨海默病中ROQUIN、Tristetraprolin(TTP)和BDNF/miR-16/TTP调节轴的分析。  |  Asadi, MR., et al. 2022. Front Aging Neurosci. 14: 933019. PMID: 36016853

    订购信息

    产品名称产品编号规格价格数量收藏夹

    TTP 抗体 (H-12)

    sc-398904
    200 µg/ml
    $322.00

    TTP (H-12): m-IgGκ BP-HRP 套装

    sc-536175
    200 µg Ab; 40 µg BP
    $361.00

    TTP (H-12): m-IgG2b BP-HRP 套装

    sc-549522
    200 µg Ab; 10 µg BP
    $361.00

    TTP (H-12) 中和勝肽

    sc-398904 P
    100 µg/0.5 ml
    $69.00

    Hello, I recently used this Ab for WB with lung homogenates from experimental mice. Even though I saw a clear band, the size of the band was unusually high (above 100kDa). Could you give me an insight about why that could be the case?

    Asked by: STlab
    Thank you for your question. We do not have any reference or data supporting that high of a molecular weight for TTP. We would recommend that you include a positive control in your Western blot testing, such as 3T3-L1, or PC-12 whole cell lysates for comparison. Please contact our Technical Service Department if you would like help troubleshooting the antibody.
    Answered by: Technical Support
    Date published: 2019-01-26

    Has this antibody been shown to detect endogenous levels of TTP in mouse Raw 264.7 cells?

    Asked by: Acarreno13
    Thank you for your question. We have not yet tested this antibody, TTP (H-12): sc-398904, in RAW 264.7 cells. However, we do have endogenous data in other mouse cell lines including RAW 309 Cr.1 and 3T3-L1 whole cell lysates.
    Answered by: Technical Support
    Date published: 2017-12-07

    What application is the blocking peptide sc-398904 P appropriate for?

    Asked by: cjMara
    Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
    Answered by: Technical Support
    Date published: 2017-02-24
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    Rated 5 out of 5 by QZ from Satisfactory Western blot data of TTP expressionSatisfactory Western blot data of TTP expression in non-transfected and human TTP transfected 293T whole cell lysates. -SCBT QC
    Date published: 2013-12-15
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    TTP 抗体 (H-12) is rated 5.0 out of 5 by 1.
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