Transcription Termination Factor 2/TTF2 Antibody (A-9): sc-393478
- Transcription Termination Factor 2/TTF2 Antibody (A-9) is a mouse monoclonal IgG1 κ Transcription Termination Factor 2/TTF2 antibody provided at 200 µg/ml
- raised against amino acids 24-173 mapping near the N-terminus of TTF2 of mouse origin
- Transcription Termination Factor 2/TTF2 Antibody (A-9) is recommended for detection of TTF2 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-Transcription Termination Factor 2/TTF2 Antibody (A-9) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for Transcription Termination Factor 2/TTF2 Antibody (A-9) for WB applications. These reagents are now offered in bundles with Transcription Termination Factor 2/TTF2 Antibody (A-9) (see ordering information below).
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TTF2 Antibody (A-9) is a mouse monoclonal IgG1 kappa light chain antibody that detects TTF2 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). TTF2 Antibody (A-9) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. TTF2, also known as transcription termination factor 2 or HuF2, is a double-stranded DNA-dependent ATPase that plays a crucial role in transcription termination by facilitating RNA polymerase II release from DNA templates. This process ensures proper gene expression and regulation by preventing transcription machinery from continuing past intended termination sites. TTF2 localizes to the cytoplasm during interphase and moves to the nucleus during mitosis, showcasing dynamic cellular functions. As a Swi2/Snf2 protein family member, TTF2 terminates transcription and participates in pre-mRNA splicing and mitotic transcriptional repression. TTF2 contains a helicase ATP-binding domain and interacts with spliceosome complex and Cdc5L (cell division cycle 5-like), highlighting multiple functions in gene regulation. Two TTF2 isoforms result from alternative splicing, potentially contributing to diverse regulatory roles across cellular contexts.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Transcription Termination Factor 2/TTF2 Antibody (A-9) References:
- hLodestar/HuF2 interacts with CDC5L and is involved in pre-mRNA splicing. | Leonard, D., et al. 2003. Biochem Biophys Res Commun. 308: 793-801. PMID: 12927788
- Involvement of transcription termination factor 2 in mitotic repression of transcription elongation. | Jiang, Y., et al. 2004. Mol Cell. 14: 375-85. PMID: 15125840
- Rescue of the TTF2 knockdown phenotype with an siRNA-resistant replacement vector. | Jiang, Y. and Price, DH. 2004. Cell Cycle. 3: 1151-3. PMID: 15467445
- Improved titanium dioxide enrichment of phosphopeptides from HeLa cells and high confident phosphopeptide identification by cross-validation of MS/MS and MS/MS/MS spectra. | Yu, LR., et al. 2007. J Proteome Res. 6: 4150-62. PMID: 17924679
- A human RNA polymerase II transcription termination factor is a SWI2/SNF2 family member. | Liu, M., et al. 1998. J Biol Chem. 273: 25541-4. PMID: 9748214
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Transcription Termination Factor 2/TTF2 Antibody (A-9) | sc-393478 | 200 µg/ml | $322.00 | |||
Transcription Termination Factor 2/TTF2 Antibody (A-9): m-IgG Fc BP-HRP Bundle | sc-527690 | 200 µg Ab; 10 µg BP | $361.00 | |||
Transcription Termination Factor 2/TTF2 Antibody (A-9): m-IgG1 BP-HRP Bundle | sc-533063 | 200 µg Ab; 20 µg BP | $361.00 | |||
Transcription Termination Factor 2/TTF2 Antibody (A-9) AC | sc-393478 AC | 500 µg/ml, 25% agarose | $424.00 | |||
Transcription Termination Factor 2/TTF2 Antibody (A-9) HRP | sc-393478 HRP | 200 µg/ml | $322.00 | |||
Transcription Termination Factor 2/TTF2 Antibody (A-9) FITC | sc-393478 FITC | 200 µg/ml | $336.00 | |||
Transcription Termination Factor 2/TTF2 Antibody (A-9) PE | sc-393478 PE | 200 µg/ml | $349.00 | |||
Transcription Termination Factor 2/TTF2 Antibody (A-9) Alexa Fluor® 488 | sc-393478 AF488 | 200 µg/ml | $364.00 | |||
Transcription Termination Factor 2/TTF2 Antibody (A-9) Alexa Fluor® 546 | sc-393478 AF546 | 200 µg/ml | $364.00 | |||
Transcription Termination Factor 2/TTF2 Antibody (A-9) Alexa Fluor® 594 | sc-393478 AF594 | 200 µg/ml | $364.00 | |||
Transcription Termination Factor 2/TTF2 Antibody (A-9) Alexa Fluor® 647 | sc-393478 AF647 | 200 µg/ml | $364.00 | |||
Transcription Termination Factor 2/TTF2 Antibody (A-9) Alexa Fluor® 680 | sc-393478 AF680 | 200 µg/ml | $364.00 | |||
Transcription Termination Factor 2/TTF2 Antibody (A-9) Alexa Fluor® 790 | sc-393478 AF790 | 200 µg/ml | $364.00 |