TS Antibody (C-5): sc-390945
- TS Antibody (C-5) is a mouse monoclonal IgG2a κ TS antibody, cited in 6 publications, provided at 200 µg/ml
- raised against amino acids 49-313 mapping at the C-terminus of TS of human origin
- TS Antibody (C-5) is recommended for detection of TS of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-TS Antibody (C-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for TS Antibody (C-5) for WB and IHC(P) applications. These reagents are now offered in bundles with TS Antibody (C-5) (see ordering information below).
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SEE ALSO...
TS Antibody (C-5) is a mouse monoclonal IgG2a kappa light chain antibody that detects TS protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Anti-TS antibody (C-5) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Thymidylate synthase (TS), also known as TYMS, TMS, TSase, and HsT422, plays a crucial role in DNA biosynthesis by catalyzing the conversion of deoxyuridine monophosphate (dUMP) to deoxythymidine monophosphate (dTMP) using 5,10-methylenetetrahydrofolate as a cofactor. This enzymatic reaction is vital for the synthesis of thymidine, which is essential for DNA replication and repair. TS functions beyond enzymatic activity as an RNA-binding protein that can interact with its own mRNA, forming a ribonucleoprotein complex that associates with other cellular mRNAs, including those of the p53 tumor suppressor gene and Myc transcription factors. This interaction highlights TS′s multifaceted role in cellular regulation and potential as a therapeutic target, particularly in cancer treatment, where TS inhibition can lead to DNA replication interference and cell death due to thymidine depletion. Cancer cells, which have a high demand for thymidine due to rapid proliferation, are particularly sensitive to TS inhibition, making anti-TS antibody (C-5) an invaluable tool for research and therapeutic applications.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
TS Antibody (C-5) References:
- Elevated levels of thymidylate synthase linked to neoplastic transformation of mammalian cells. | Voeller, D., et al. 2004. Cell Cycle. 3: 1005-7. PMID: 15280655
- Leucovorin enhancement of the effects of the fluoropyrimidines on thymidylate synthase. | Morgan, RG. 1989. Cancer. 63: 1008-12. PMID: 2521810
- Predictive modeling targets thymidylate synthase ThyX in Mycobacterium tuberculosis. | Djaout, K., et al. 2016. Sci Rep. 6: 27792. PMID: 27283217
- Thymidylate synthase gene polymorphisms as important contributors affecting hepatocellular carcinoma prognosis. | Wang, X., et al. 2017. Clin Res Hepatol Gastroenterol. 41: 319-326. PMID: 28043790
- Topoisomerase 2α and thymidylate synthase expression in adrenocortical cancer. | Roca, E., et al. 2017. Endocr Relat Cancer. 24: 319-327. PMID: 28432084
- Thymidylate Synthase, a New Myoepithelial Biomarker for Breast Lesions. | Guo, R., et al. 2019. Int J Surg Pathol. 27: 852-858. PMID: 31234664
- Diagnostic and prognostic value of thymidylate synthase expression in breast cancer. | Song, S., et al. 2021. Clin Exp Pharmacol Physiol. 48: 279-287. PMID: 33030246
- Positive Cooperativity in Substrate Binding by Human Thymidylate Synthase. | Bonin, JP., et al. 2021. Biophys J. 120: 4137. PMID: 34433070
- Upregulation of Thymidylate Synthase Induces Pemetrexed Resistance in Malignant Pleural Mesothelioma. | Sato, Y., et al. 2021. Front Pharmacol. 12: 718675. PMID: 34646134
- Gastric cancer with microsatellite instability displays increased thymidylate synthase expression. | Pereira, MA., et al. 2022. J Surg Oncol. 126: 116-124. PMID: 35689583
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TS Antibody (C-5) | sc-390945 | 200 µg/ml | $322.00 | |||
TS Antibody (C-5): m-IgG Fc BP-HRP Bundle | sc-530310 | 200 µg Ab; 10 µg BP | $361.00 | |||
TS Antibody (C-5): m-IgGκ BP-HRP Bundle | sc-523746 | 200 µg Ab, 40 µg BP | $361.00 | |||
TS Antibody (C-5) AC | sc-390945 AC | 500 µg/ml, 25% agarose | $424.00 | |||
TS Antibody (C-5) HRP | sc-390945 HRP | 200 µg/ml | $322.00 | |||
TS Antibody (C-5) FITC | sc-390945 FITC | 200 µg/ml | $336.00 | |||
TS Antibody (C-5) PE | sc-390945 PE | 200 µg/ml | $349.00 | |||
TS Antibody (C-5) Alexa Fluor® 488 | sc-390945 AF488 | 200 µg/ml | $364.00 | |||
TS Antibody (C-5) Alexa Fluor® 546 | sc-390945 AF546 | 200 µg/ml | $364.00 | |||
TS Antibody (C-5) Alexa Fluor® 594 | sc-390945 AF594 | 200 µg/ml | $364.00 | |||
TS Antibody (C-5) Alexa Fluor® 647 | sc-390945 AF647 | 200 µg/ml | $364.00 | |||
TS Antibody (C-5) Alexa Fluor® 680 | sc-390945 AF680 | 200 µg/ml | $364.00 | |||
TS Antibody (C-5) Alexa Fluor® 790 | sc-390945 AF790 | 200 µg/ml | $364.00 |