TRUB2 Antibody (E-6): sc-514573
- TRUB2 Antibody (E-6) is a mouse monoclonal IgG2b κ TRUB2 antibody provided at 200 µg/ml
- raised against amino acids 141-309 mapping near the C-terminus of TRUB2 of human origin
- TRUB2 Antibody (E-6) is recommended for detection of TRUB2 of human origin by WB, IP, IF and ELISA
- Anti-TRUB2 Antibody (E-6) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for TRUB2 Antibody (E-6) for WB applications. These reagents are now offered in bundles with TRUB2 Antibody (E-6) (see ordering information below).
QUICK LINKS
SEE ALSO...
TRUB2 Antibody (E-6) is a mouse monoclonal IgG2b kappa light chain antibody that detects TRUB2 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). TRUB2 (E-6) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. TRUB2, also known as tRNA pseudouridine synthase 2, is a 331 amino acid enzyme that plays a crucial role in the post-transcriptional modification of tRNA by catalyzing the conversion of uracil to pseudouridine, a modification essential for tRNA stability and function. This modification is important because pseudouridine enhances tRNA structural integrity, improving protein synthesis accuracy and overall cellular function. TRUB2 contains a TruB domain and exhibits approximately 30% sequence similarity with TRUB1, indicating a shared evolutionary origin linked to eubacterial tRNA synthases, while being distinct from Dyskerin. The gene encoding TRUB2 is located on human chromosome 9, a region housing over 900 genes and associated with hereditary hemorrhagic telangiectasia and familial dysautonomia, conditions linked to vascular defects and autonomic nervous system dysfunction. Additionally, chromosome 9 contains the largest cluster of interferon family genes, underscoring its importance in immune response regulation.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
TRUB2 Antibody (E-6) References:
- Physiological analysis of the role of truB in Escherichia coli: a role for tRNA modification in extreme temperature resistance. | Kinghorn, SM., et al. 2002. Microbiology (Reading). 148: 3511-3520. PMID: 12427942
- The human TruB family of pseudouridine synthase genes, including the Dyskeratosis Congenita 1 gene and the novel member TRUB1. | Zucchini, C., et al. 2003. Int J Mol Med. 11: 697-704. PMID: 12736709
- Structure of tRNA pseudouridine synthase TruB and its RNA complex: RNA recognition through a combination of rigid docking and induced fit. | Pan, H., et al. 2003. Proc Natl Acad Sci U S A. 100: 12648-53. PMID: 14566049
- Formation of the conserved pseudouridine at position 55 in archaeal tRNA. | Roovers, M., et al. 2006. Nucleic Acids Res. 34: 4293-301. PMID: 16920741
- Differential roles of archaeal box H/ACA proteins in guide RNA-dependent and independent pseudouridine formation. | Gurha, P., et al. 2007. RNA Biol. 4: 101-9. PMID: 17993784
- Purification, cloning, and properties of the tRNA psi 55 synthase from Escherichia coli. | Nurse, K., et al. 1995. RNA. 1: 102-12. PMID: 7489483
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TRUB2 Antibody (E-6) | sc-514573 | 200 µg/ml | $322.00 | |||
TRUB2 Antibody (E-6): m-IgG Fc BP-HRP Bundle | sc-531165 | 200 µg Ab; 10 µg BP | $361.00 | |||
TRUB2 Antibody (E-6): m-IgGκ BP-HRP Bundle | sc-524775 | 200 µg Ab, 40 µg BP | $361.00 | |||
TRUB2 Antibody (E-6): m-IgG2b BP-HRP Bundle | sc-550263 | 200 µg Ab; 10 µg BP | $361.00 | |||
TRUB2 Antibody (E-6) AC | sc-514573 AC | 500 µg/ml, 25% agarose | $424.00 | |||
TRUB2 Antibody (E-6) HRP | sc-514573 HRP | 200 µg/ml | $322.00 | |||
TRUB2 Antibody (E-6) FITC | sc-514573 FITC | 200 µg/ml | $336.00 | |||
TRUB2 Antibody (E-6) PE | sc-514573 PE | 200 µg/ml | $349.00 | |||
TRUB2 Antibody (E-6) Alexa Fluor® 488 | sc-514573 AF488 | 200 µg/ml | $364.00 | |||
TRUB2 Antibody (E-6) Alexa Fluor® 546 | sc-514573 AF546 | 200 µg/ml | $364.00 | |||
TRUB2 Antibody (E-6) Alexa Fluor® 594 | sc-514573 AF594 | 200 µg/ml | $364.00 | |||
TRUB2 Antibody (E-6) Alexa Fluor® 647 | sc-514573 AF647 | 200 µg/ml | $364.00 | |||
TRUB2 Antibody (E-6) Alexa Fluor® 680 | sc-514573 AF680 | 200 µg/ml | $364.00 | |||
TRUB2 Antibody (E-6) Alexa Fluor® 790 | sc-514573 AF790 | 200 µg/ml | $364.00 |