Trk A Antibody (Y32Ex) recommended for detection of Trk A of human origin by WB

Trk A Antibody (Y32Ex): sc-80398

Trk A Antibody (Y32Ex) is rated 1.5 out of 5 by 2.
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Datasheets

    • Anti-Trk A Antibody (Y32Ex) is a mouse monoclonal IgG2a (kappa light chain) Trk A antibody provided at 100 µg/ml
    • raised against an extracellular domain of Trk A of human origin
    • recommended for detection of Trk A of human origin by WB; non cross-reactive with Trk B or Trk C
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • See Trk (B-3): sc-7268 for Trk A antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Trk A (Y32Ex): sc-80398.

How much sample do you recommend loading on the gel for Western blot detection using Trk A (Y32Ex): sc-80398?

Asked by: Dr Ninau Qelp
We recommend loading 40–60 µg whole cell lysate, 10–20 µg nuclear extract, 5-10 µg transfected lysate, or 10–20 ng purified protein per lane. A link to our complete Western blotting protocol is provided here for your reference. https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-27
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Rated 1 out of 5 by from TrkA antibody for WB The antibody is just terrible! There is no band whatsoever!
Date published: 2017-04-05
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