TPR Antibody (H-8): sc-271565

TPR Antibody (H-8) is a mouse monoclonal IgG2b kappa light chain antibody that detects TPR protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-TPR antibody (H-8) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. TPR protein plays a crucial role in the structure and function of the vertebrate nuclear pore complex (NPC), which is essential for regulating the transport of molecules between the nucleus and cytoplasm. NPC is a large macromolecular assembly that consists of multiple protein subcomplexes arranged in a symmetrical structure, facilitating selective exchange of proteins and RNA. TPR is localized to intranuclear filaments of NPC and is involved in import and export processes of nuclear proteins, acting as a critical component of cytoplasmic fibrils that mediate these transport functions. TPR′s unique coiled-coil domain allows anchoring to NPC, while TPR′s acidic COOH terminus is free to interact with soluble transport factors, thereby influencing export of macromolecules from the nucleus. This intricate interplay underscores TPR′s importance in maintaining cellular homeostasis and regulating gene expression through involvement in nucleocytoplasmic transport.

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TPR Antibody (H-8) References:

1. TPR proteins: the versatile helix.  |  D'Andrea, LD. and Regan, L. 2003. Trends Biochem Sci. 28: 655-62. PMID: 14659697
2. Nucleoporins as components of the nuclear pore complex core structure and Tpr as the architectural element of the nuclear basket.  |  Krull, S., et al. 2004. Mol Biol Cell. 15: 4261-77. PMID: 15229283
3. Large-scale characterization of HeLa cell nuclear phosphoproteins.  |  Beausoleil, SA., et al. 2004. Proc Natl Acad Sci U S A. 101: 12130-5. PMID: 15302935
4. Versatile TPR domains accommodate different modes of target protein recognition and function.  |  Allan, RK. and Ratajczak, T. 2011. Cell Stress Chaperones. 16: 353-67. PMID: 21153002
5. TPR-containing proteins control protein organization and homeostasis for the endoplasmic reticulum.  |  Graham, JB., et al. 2019. Crit Rev Biochem Mol Biol. 54: 103-118. PMID: 31023093
6. TPR is required for the efficient nuclear export of mRNAs and lncRNAs from short and intron-poor genes.  |  Lee, ES., et al. 2020. Nucleic Acids Res. 48: 11645-11663. PMID: 33091126
7. Cancer-related SRCAP and TPR mutations in colon cancers.  |  Moon, SW., et al. 2021. Pathol Res Pract. 217: 153292. PMID: 33307343
8. The TPR domain of PgaA is a multifunctional scaffold that binds PNAG and modulates PgaB-dependent polymer processing.  |  Pfoh, R., et al. 2022. PLoS Pathog. 18: e1010750. PMID: 35930610
9. CircZCCHC2 decreases pirarubicin sensitivity and promotes triple-negative breast cancer development via the miR-1200/TPR axis.  |  Zhang, F., et al. 2024. iScience. 27: 109057. PMID: 38361605
10. Tpr, a large coiled coil protein whose amino terminus is involved in activation of oncogenic kinases, is localized to the cytoplasmic surface of the nuclear pore complex.  |  Byrd, DA., et al. 1994. J Cell Biol. 127: 1515-26. PMID: 7798308
11. Molecular segments of protein Tpr that confer nuclear targeting and association with the nuclear pore complex.  |  Cordes, VC., et al. 1998. Exp Cell Res. 245: 43-56. PMID: 9828100
12. Functional analysis of Tpr: identification of nuclear pore complex association and nuclear localization domains and a role in mRNA export.  |  Bangs, P., et al. 1998. J Cell Biol. 143: 1801-12. PMID: 9864356

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-TPR Antibody (H-8) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-TPR Antibody (H-8) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

TPR Antibody (H-8) Specifications

Alternate Product Name: TPR

Product Clone Name: H-8

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 1-300

Epitope Target: TPR

Epitope Species: human

Product Reactivity/Detection: TPR

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for TPR Antibody (H-8): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin