TPPP Antibody (A-6): sc-515819

TPPP Antibody (A-6) is a mouse monoclonal IgG1 kappa light chain antibody that detects TPPP protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-TPPP antibody (A-6) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. TPPP protein, also known as tubulin polymerization-promoting protein, p24, or p25, is a 219 amino acid protein predominantly located in the perinuclear region of the cytoplasm, where TPPP plays a crucial role in the assembly of microtubules. Microtubules are essential structural components that facilitate vital cellular processes such as mitosis, cytokinesis, and vesicle transport. TPPP functions by promoting the polymerization of tubulin into various structures, including double-walled tubules and stabilized aggregates, which are critical for maintaining cellular integrity during division. Notably, overexpression of TPPP can disrupt the formation of the mitotic spindle and hinder the breakdown of the nuclear envelope, highlighting TPPP′s importance in cell cycle regulation. Additionally, TPPP is subject to phosphorylation by TPK II, a modification that can influence TPPP′s activity and interactions. The gene encoding TPPP is located on human chromosome 5, which encompasses 181 million base pairs and represents nearly 6% of the human genome, underscoring TPPP′s evolutionary significance and potential implications in various biological processes.

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TPPP Antibody (A-6) References:

1. P25alpha/Tubulin polymerization promoting protein expression by myelinating oligodendrocytes of the developing rat brain.  |  Skjoerringe, T., et al. 2006. J Neurochem. 99: 333-42. PMID: 16879710
2. Tubulin polymerization promoting proteins (TPPPs): members of a new family with distinct structures and functions.  |  Vincze, O., et al. 2006. Biochemistry. 45: 13818-26. PMID: 17105200
3. TPPP/p25 in brain tumours: expression in non-neoplastic oligodendrocytes but not in oligodendroglioma cells.  |  Preusser, M., et al. 2007. Acta Neuropathol. 113: 213-5. PMID: 17136548
4. A new entry to polycyclic indole derivatives via intramolecular imino Diels-Alder reaction: observation of unexpected reaction.  |  Gaddam, V. and Nagarajan, R. 2007. J Org Chem. 72: 3573-6. PMID: 17408284
5. A quantitative atlas of mitotic phosphorylation.  |  Dephoure, N., et al. 2008. Proc Natl Acad Sci U S A. 105: 10762-7. PMID: 18669648
6. [Lipodystrophia diabetica].  |  Libman, E., et al. 1981. Med Pregl. 34: 49-53. PMID: 7027006

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-TPPP Antibody (A-6) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-TPPP Antibody (A-6) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

TPPP Antibody (A-6) Specifications

Alternate Product Name: TPPP

Product Clone Name: A-6

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-77

Epitope Target: TPPP

Epitope Species: human

Product Reactivity/Detection: TPPP

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for TPPP Antibody (A-6): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin