TPO Antibody (A-6): sc-514326

TPO Antibody (A-6) is a mouse monoclonal IgG1 antibody that detects thrombopoietin (TPO) in mouse samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). TPO is a crucial cytokine that regulates megakaryopoiesis, the process of platelet production, by promoting the development and maturation of megakaryocytes. TPO is predominantly expressed in mature and progenitor megakaryocytes, as well as in human platelets, highlighting TPO′s essential role in hematopoiesis. The mature TPO protein is derived from a 332 amino acid precursor, which includes a 21 amino acid signal peptide that is cleaved during processing. TPO is highly glycosylated, as evidenced by the variation in molecular weight observed in serum samples, which can influence TPO′s stability and activity. TPO exerts biological effects by binding to the c-Mpl receptor, leading to the activation of Janus tyrosine kinases, specifically Tyk 2 and JAK2. This activation triggers the phosphorylation of Stat5 and Stat3, facilitating their translocation to the nucleus where they promote the transcription of genes responsive to Stat signaling. The gene encoding TPO is located on human chromosome 3q26.3-q27, underscoring TPO′s genetic significance in the regulation of platelet homeostasis. Anti-TPO antibody (A-6) is an invaluable tool for researchers studying the mechanisms of megakaryocyte development and platelet production in mouse models.

The preferred TPO antibody is TPO Antibody (F-3): sc-374045.