TPO Antibody (F-3): sc-374045

TPO Antibody (F-3) is a mouse monoclonal IgG1 kappa light chain antibody that detects TPO protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). TPO Antibody (F-3) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Thrombopoietin (TPO), also known as c-Mpl ligand, is a crucial cytokine that significantly influences megakaryopoiesis, the process of platelet production from megakaryocytes. TPO is primarily expressed in mature and progenitor megakaryocytes, as well as in human platelets, highlighting its essential role in hematopoiesis. The mature TPO protein is derived from a 332 amino acid precursor, which includes a 21 amino acid signal peptide that is cleaved during processing. TPO exhibits a range of molecular weights in serum, indicating extensive glycosylation, which is vital for stability and activity. TPO interaction with c-Mpl receptor triggers a cascade of intracellular signaling through the activation of Janus kinase family members, including Tyk 2 and JAK2, leading to Stat5 and Stat3 phosphorylation. This phosphorylation facilitates nuclear translocation of these transcription factors, ultimately promoting the expression of genes responsive to Stat signaling. TPO gene is located on human chromosome 3q26.3-q27, underscoring genetic significance in platelet production regulation and overall hematological health.

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TPO Antibody (F-3) References:

1. TPO/c-mpl ligand induces tyrosine phosphorylation of multiple cellular proteins including proto-oncogene products, Vav and c-Cbl, and Ras signaling molecules.  |  Sasaki, K., et al. 1995. Biochem Biophys Res Commun. 216: 338-47. PMID: 7488109
2. Regulation of platelet activation in vitro by the c-Mpl ligand, thrombopoietin.  |  Chen, J., et al. 1995. Blood. 86: 4054-62. PMID: 7492761
3. Thrombopoietin (TPO) induces tyrosine phosphorylation and activation of STAT5 and STAT3.  |  Bacon, CM., et al. 1995. FEBS Lett. 370: 63-8. PMID: 7544303
4. TPO and IL-3 induce overlapping but distinct protein tyrosine phosphorylation in a myeloid precursor cell line.  |  Dorsch, M., et al. 1995. Biochem Biophys Res Commun. 214: 424-31. PMID: 7545904
5. Thrombopoietin, c-Mpl ligand, induces tyrosine phosphorylation of Tyk2, JAK2, and STAT3, and enhances agonists-induced aggregation in platelets in vitro.  |  Ezumi, Y., et al. 1995. FEBS Lett. 374: 48-52. PMID: 7589510
6. Thrombopoietin: the primary regulator of platelet production.  |  Kaushansky, K. 1995. Blood. 86: 419-31. PMID: 7605981
7. Human thrombopoietin: gene structure, cDNA sequence, expression, and chromosomal localization.  |  Foster, DC., et al. 1994. Proc Natl Acad Sci U S A. 91: 13023-7. PMID: 7809166
8. Megakaryocyte growth and development factor and interleukin-3 induce patterns of protein-tyrosine phosphorylation that correlate with dominant differentiation over proliferation of mpl-transfected 32D cells.  |  Mu, SX., et al. 1995. Blood. 86: 4532-43. PMID: 8541543

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-TPO Antibody (F-3) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-TPO Antibody (F-3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

TPO Antibody (F-3) Specifications

Alternate Product Name: TPO

Product Clone Name: F-3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 29-52

Epitope Target: TPO

Epitope Species: human

Product Reactivity/Detection: precursor and mature TPO

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: porcine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for TPO Antibody (F-3): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin