TORC1 Antibody (H-6) recommended for detection of TORC1 of human origin by WB, IP, IF and ELISA

TORC1 Antibody (H-6): sc-365010

TORC1 Antibody (H-6) is rated 5.0 out of 5 by 2.
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Datasheets

    • TORC1 Antibody (H-6) is a mouse monoclonal IgG2b (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 391-515 mapping within an internal region of TORC1 of human origin
    • recommended for detection of TORC1 of human origin by WB, IP, IF and ELISA
    • TransCruz reagent for ChIP application (sc-365010 X, 200 µg/0.1 ml)
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.

What is the recommended fixation method for immunofluorescence staining with TORC1 (H-6): sc-365010 monoclonal antibody?

Asked by: Trav11
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-03-01
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Rated 5 out of 5 by from Produced superb immunofluorescence nuclear Produced superb immunofluorescence nuclear and cytoplasmic staining in formalin-fixed HepG2 cells. -SCBT QC
Date published: 2015-01-28
Rated 5 out of 5 by from Produced positive Western Blot data of TORC1 Produced positive Western Blot data of TORC1 expression in non-transfected and human TORC1 transfected 293T whole cell lysates. -SCBT QC
Date published: 2014-03-11
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