Tom40 Antibody (H-7): sc-365466

Tom40 Antibody (H-7) is a mouse monoclonal IgG2b light chain antibody that detects Tom40 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Tom40 is a crucial component of the mitochondrial preprotein translocase of the outer membrane (Tom) complex, which plays a vital role in the import of nucleus-encoded precursor proteins into mitochondria. Tom40 forms the general import pore (GIP) that allows various preproteins to traverse the mitochondrial outer membrane, thereby facilitating essential mitochondrial functions such as energy production and metabolic regulation. The structure of Tom40 consists of a β-barrel configuration that creates a channel through which preproteins can pass. This structural feature ensures selective transport of proteins while maintaining the integrity of the mitochondrial membrane, critical for cellular homeostasis. The interaction of Tom40 with other components of the Tom complex, such as Tom22 and Tom20, is essential for efficient translocation of preproteins, highlighting the importance of structural integrity in mitochondrial biogenesis and function. Anti-Tom40 antibody (H-7) serves as an invaluable tool for researchers studying mitochondrial dynamics and protein import mechanisms, providing insights into the molecular underpinnings of mitochondrial-related diseases.

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Tom40 Antibody (H-7) References:

1. Biogenesis of Tom40, core component of the TOM complex of mitochondria.  |  Rapaport, D. and Neupert, W. 1999. J Cell Biol. 146: 321-31. PMID: 10427088
2. Structural requirements of Tom40 for assembly into preexisting TOM complexes of mitochondria.  |  Rapaport, D., et al. 2001. Mol Biol Cell. 12: 1189-98. PMID: 11359915
3. Tom40, the pore-forming component of the protein-conducting TOM channel in the outer membrane of mitochondria.  |  Ahting, U., et al. 2001. J Cell Biol. 153: 1151-60. PMID: 11402060
4. Effect of mutations in Tom40 on stability of the translocase of the outer mitochondrial membrane (TOM) complex, assembly of Tom40, and import of mitochondrial preproteins.  |  Sherman, EL., et al. 2006. J Biol Chem. 281: 22554-65. PMID: 16757481
5. Assembly of the mitochondrial protein import channel: role of Tom5 in two-stage interaction of Tom40 with the SAM complex.  |  Becker, T., et al. 2010. Mol Biol Cell. 21: 3106-13. PMID: 20668160
6. The Tom40 assembly process probed using the attachment of different intramitochondrial sorting signals.  |  Shiota, T., et al. 2012. Mol Biol Cell. 23: 3936-47. PMID: 22933571
7. TOM40 Targets Atg2 to Mitochondria-Associated ER Membranes for Phagophore Expansion.  |  Tang, Z., et al. 2019. Cell Rep. 28: 1744-1757.e5. PMID: 31412244
8. Exosome mediated Tom40 delivery protects against hydrogen peroxide-induced oxidative stress by regulating mitochondrial function.  |  Sayeed, N. and Sugaya, K. 2022. PLoS One. 17: e0272511. PMID: 35951602
9. TOM40 regulates the progression of nasopharyngeal carcinoma through ROS-mediated AKT/mTOR and p53 signaling.  |  Ran, H., et al. 2023. Discov Oncol. 14: 109. PMID: 37351718
10. TOM40 mediates the effect of TSPO on postpartum depression partially through regulating calcium homeostasis in microglia.  |  Zhai, DS., et al. 2024. J Affect Disord. 348: 283-296. PMID: 38159656

The preferred Tom40 antibody is Tom40 Antibody (D-2): sc-365467.