Tom40 Antibody (D-2): sc-365467

Tom40 Antibody (D-2) is a mouse monoclonal IgG1 kappa light chain antibody that detects Tom40 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Tom40 (D-2) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Tom40 plays a crucial role in mitochondrial function as a key component of the translocase of the outer mitochondrial membrane (TOM) complex, which is essential for importing nucleus-encoded precursor proteins into mitochondria. Tom40 forms a general import pore in the outer mitochondrial membrane that allows translocation of various preproteins. Tom40 ensures necessary proteins are imported into mitochondria for critical processes like oxidative phosphorylation and metabolic regulation, making Tom40 vital for mitochondrial biogenesis and energy production. Tom40 interacts with other proteins in the TOM complex, including Tom20 and Tom22, which recognize and bind precursor proteins to facilitate transport into mitochondria. Efficient incorporation of Tom40 into the TOM complex maintains mitochondrial integrity and function, highlighting Tom40′s importance in cellular energy metabolism and overall cellular health.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Tom40 Antibody (D-2) References:

1. Distribution of binding sequences for the mitochondrial import receptors Tom20, Tom22, and Tom70 in a presequence-carrying preprotein and a non-cleavable preprotein.  |  Brix, J., et al. 1999. J Biol Chem. 274: 16522-30. PMID: 10347216
2. Biogenesis of Tom40, core component of the TOM complex of mitochondria.  |  Rapaport, D. and Neupert, W. 1999. J Cell Biol. 146: 321-31. PMID: 10427088
3. Tom22 is a multifunctional organizer of the mitochondrial preprotein translocase.  |  van Wilpe, S., et al. 1999. Nature. 401: 485-9. PMID: 10519552
4. The TOM core complex: the general protein import pore of the outer membrane of mitochondria.  |  Ahting, U., et al. 1999. J Cell Biol. 147: 959-68. PMID: 10579717
5. The transport machinery for the import of preproteins across the outer mitochondrial membrane.  |  Ryan, MT., et al. 2000. Int J Biochem Cell Biol. 32: 13-21. PMID: 10661891
6. Presequence recognition by the tom40 channel contributes to precursor translocation into the mitochondrial matrix.  |  Melin, J., et al. 2014. Mol Cell Biol. 34: 3473-85. PMID: 25002531
7. The Broad Impact of TOM40 on Neurodegenerative Diseases in Aging.  |  Gottschalk, WK., et al. 2014. J Parkinsons Dis Alzheimers Dis. 1: PMID: 25745640
8. TOM40 as a prognostic oncogene for oral squamous cell carcinoma prognosis.  |  Deng, L., et al. 2025. BMC Cancer. 25: 92. PMID: 39815211
9. Mitochondrial protein import. Tom40 plays a major role in targeting and translocation of preproteins by forming a specific binding site for the presequence.  |  Rapaport, D., et al. 1997. J Biol Chem. 272: 18725-31. PMID: 9228044
10. Functional analysis of human mitochondrial receptor Tom20 for protein import into mitochondria.  |  Yano, M., et al. 1998. J Biol Chem. 273: 26844-51. PMID: 9756929
11. Tom40 forms the hydrophilic channel of the mitochondrial import pore for preproteins [see comment].  |  Hill, K., et al. 1998. Nature. 395: 516-21. PMID: 9774109
12. Preprotein translocase of the outer mitochondrial membrane: molecular dissection and assembly of the general import pore complex.  |  Dekker, PJ., et al. 1998. Mol Cell Biol. 18: 6515-24. PMID: 9774667

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Tom40 Antibody (D-2) has 47 citations in a variety of scientific publications.

Primary antibodies like Anti-Tom40 Antibody (D-2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Tom40 Antibody (D-2) Specifications

Alternate Product Name: TOMM40

Product Clone Name: D-2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 62-361

Epitope Target: Tom40

Epitope Species: human

Product Reactivity/Detection: Tom40

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 47 citations in various scientific publications

Available Conjugates/Forms for Tom40 Antibody (D-2): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin