TOB1 Antibody (E-1): sc-133095

TOB1 Antibody (E-1) is a mouse monoclonal IgG2a kappa light chain antibody that detects TOB1 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). TOB1 (E-1) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. TOB1, also known as TROB or APRO6, plays a crucial role in regulating cell cycle progression, particularly in the transition from G0/G1 phase to S phase, through interactions with the mammalian homologue of yeast Caf1. This regulation is vital for maintaining proper cellular proliferation and function, especially in T lymphocytes, where TOB1 can interact with Smad2/4 to enhance SMAD DNA binding to the interleukin-2 promoter, ultimately inhibiting IL-2 transcription. Additionally, TOB1′s phosphorylation at specific serine residues by ERK1/2 upon growth-factor stimulation highlights involvement in signaling pathways that influence cell growth and differentiation. The TOB2 gene, which encodes a related protein, exhibits high expression levels in tissues such as skeletal muscle, thymus, and ovary, further underscoring the importance of TOB proteins in various biological processes.

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LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

TOB1 Antibody (E-1) References:

1. Tob2, a novel anti-proliferative Tob/BTG1 family member, associates with a component of the CCR4 transcriptional regulatory complex capable of binding cyclin-dependent kinases.  |  Ikematsu, N., et al. 1999. Oncogene. 18: 7432-41. PMID: 10602502
2. Cloning and characterization of the mouse tob2 gene.  |  Ajima, R., et al. 2000. Gene. 253: 215-20. PMID: 10940559
3. Negative regulation of BMP/Smad signaling by Tob in osteoblasts.  |  Yoshida, Y., et al. 2000. Cell. 103: 1085-97. PMID: 11163184
4. Tob is a negative regulator of activation that is expressed in anergic and quiescent T cells.  |  Tzachanis, D., et al. 2001. Nat Immunol. 2: 1174-82. PMID: 11694881
5. Phosphorylation of three regulatory serines of Tob by Erk1 and Erk2 is required for Ras-mediated cell proliferation and transformation.  |  Suzuki, T., et al. 2002. Genes Dev. 16: 1356-70. PMID: 12050114
6. Identification of the Anti-proliferative protein Tob as a MAPK substrate.  |  Maekawa, M., et al. 2002. J Biol Chem. 277: 37783-7. PMID: 12151396
7. Nuclear localization of Tob is important for regulation of its antiproliferative activity.  |  Kawamura-Tsuzuku, J., et al. 2004. Oncogene. 23: 6630-8. PMID: 15235587
8. The negative cell cycle regulator, Tob (transducer of ErbB-2), is a multifunctional protein involved in hippocampus-dependent learning and memory.  |  Jin, M., et al. 2005. Neuroscience. 131: 647-59. PMID: 15730870
9. A genome-wide CRISPR screening uncovers that TOB1 acts as a key host factor for FMDV infection via both IFN and EGFR mediated pathways.  |  Peng, G., et al. 2024. PLoS Pathog. 20: e1012104. PMID: 38512977
10. TOB1 modulates neutrophil phenotypes to influence gastric cancer progression and immunotherapy efficacy.  |  Zhang, J., et al. 2024. Front Immunol. 15: 1369087. PMID: 38617839
11. Loss of Tob1 promotes muscle regeneration through muscle stem cell expansion.  |  Kitajima, Y., et al. 2024. J Cell Sci. 137: PMID: 39037211
12. Tob, a novel protein that interacts with p185erbB2, is associated with anti-proliferative activity.  |  Matsuda, S., et al. 1996. Oncogene. 12: 705-13. PMID: 8632892

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-TOB1 Antibody (E-1) has 7 citations in a variety of scientific publications.

Primary antibodies like Anti-TOB1 Antibody (E-1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

TOB1 Antibody (E-1) Specifications

Alternate Product Name: TOB1

Product Clone Name: E-1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 271-340

Epitope Target: TOB1

Epitope Species: human

Product Reactivity/Detection: TOB1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 7 citations in various scientific publications

Available Conjugates/Forms for TOB1 Antibody (E-1): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin